ID   Q9CPS5_MOUSE            Unreviewed;       289 AA.
AC   Q9CPS5; Q8BMV2;
DT   01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 2.
DT   02-SEP-2026, entry version 158.
DE   RecName: Full=26S proteasome non-ATPase regulatory subunit 8 {ECO:0000256|ARBA:ARBA00014939};
DE   AltName: Full=26S proteasome regulatory subunit RPN12 {ECO:0000256|ARBA:ARBA00078986};
GN   Name=Psmd8 {ECO:0000313|MGI:MGI:1888669};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:BAB22789.2};
RN   [1] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA   Carninci P., Hayashizaki Y.;
RT   "High-efficiency full-length cDNA cloning.";
RL   Methods Enzymol. 303:19-44(1999).
RN   [2] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=11042159; DOI=10.1101/gr.145100;
RA   Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA   Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT   "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT   full-length cDNA libraries for rapid discovery of new genes.";
RL   Genome Res. 10:1617-1630(2000).
RN   [3] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=11076861; DOI=10.1101/gr.152600;
RA   Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA   Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA   Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA   Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA   Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA   Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA   Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT   "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT   pipeline with 384 multicapillary sequencer.";
RL   Genome Res. 10:1757-1771(2000).
RN   [4] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Kidney {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RA   Adachi J., Aizawa K., Akahira S., Akimura T., Arai A., Aono H., Arakawa T.,
RA   Bono H., Carninci P., Fukuda S., Fukunishi Y., Furuno M., Hanagaki T.,
RA   Hara A., Hayatsu N., Hiramoto K., Hiraoka T., Hori F., Imotani K.,
RA   Ishii Y., Itoh M., Izawa M., Kasukawa T., Kato H., Kawai J., Kojima Y.,
RA   Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T., Miyazaki A.,
RA   Nishi K., Nomura K., Numazaki R., Ohno M., Okazaki Y., Okido T., Owa C.,
RA   Saito H., Saito R., Sakai C., Sakai K., Sano H., Sasaki D., Shibata K.,
RA   Shibata Y., Shinagawa A., Shiraki T., Sogabe Y., Suzuki H., Tagami M.,
RA   Tagawa A., Takahashi F., Tanaka T., Tejima Y., Toya T., Yamamura T.,
RA   Yasunishi A., Yoshida K., Yoshino M., Muramatsu M., Hayashizaki Y.;
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=11217851; DOI=10.1038/35055500;
RG   The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT   "Functional annotation of a full-length mouse cDNA collection.";
RL   Nature 409:685-690(2001).
RN   [6] {ECO:0000313|EMBL:BAC25683.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAC25683.1};
RC   TISSUE=Whole body {ECO:0000313|EMBL:BAC25683.1};
RA   Adachi J., Aizawa K., Akimura T., Arakawa T., Bono H., Carninci P.,
RA   Fukuda S., Furuno M., Hanagaki T., Hara A., Hashizume W., Hayashida K.,
RA   Hayatsu N., Hiramoto K., Hiraoka T., Hirozane T., Hori F., Imotani K.,
RA   Ishii Y., Itoh M., Kagawa I., Kasukawa T., Katoh H., Kawai J., Kojima Y.,
RA   Kondo S., Konno H., Kouda M., Koya S., Kurihara C., Matsuyama T.,
RA   Miyazaki A., Murata M., Nakamura M., Nishi K., Nomura K., Numazaki R.,
RA   Ohno M., Ohsato N., Okazaki Y., Saito R., Saitoh H., Sakai C., Sakai K.,
RA   Sakazume N., Sano H., Sasaki D., Shibata K., Shinagawa A., Shiraki T.,
RA   Sogabe Y., Tagami M., Tagawa A., Takahashi F., Takaku-Akahira S.,
RA   Takeda Y., Tanaka T., Tomaru A., Toya T., Yasunishi A., Muramatsu M.,
RA   Hayashizaki Y.;
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=12466851; DOI=10.1038/nature01266;
RG   The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT   "Analysis of the mouse transcriptome based on functional annotation of
RT   60,770 full-length cDNAs.";
RL   Nature 420:563-573(2002).
RN   [8] {ECO:0000313|EMBL:BAE29609.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAE29609.1};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1};
RA   Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F.,
RA   Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M.,
RA   Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H., Nomura K.,
RA   Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T.,
RA   Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.;
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [9] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RG   The FANTOM Consortium;
RG   Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT   "The Transcriptional Landscape of the Mammalian Genome.";
RL   Science 309:1559-1563(2005).
RN   [10] {ECO:0000313|EMBL:BAB22789.2}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=C57BL/6J {ECO:0000313|EMBL:BAB22789.2};
RC   TISSUE=Bone marrow {ECO:0000313|EMBL:BAE29609.1}, Kidney
RC   {ECO:0000313|EMBL:BAB22458.2}, and Whole body
RC   {ECO:0000313|EMBL:BAB22789.2};
RX   PubMed=16141073; DOI=10.1126/science.1112009;
RG   RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT   "Antisense Transcription in the Mammalian Transcriptome.";
RL   Science 309:1564-1566(2005).
RN   [11] {ECO:0007829|PubMed:21183079}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Component of the 26S proteasome, a multiprotein complex
CC       involved in the ATP-dependent degradation of ubiquitinated proteins.
CC       This complex plays a key role in the maintenance of protein homeostasis
CC       by removing misfolded or damaged proteins, which could impair cellular
CC       functions, and by removing proteins whose functions are no longer
CC       required. Therefore, the proteasome participates in numerous cellular
CC       processes, including cell cycle progression, apoptosis, or DNA damage
CC       repair. {ECO:0000256|ARBA:ARBA00002362}.
CC   -!- SUBUNIT: Component of the 19S proteasome regulatory particle complex.
CC       The 26S proteasome consists of a 20S core particle (CP) and two 19S
CC       regulatory subunits (RP). The regulatory particle is made of a lid
CC       composed of 9 subunits including PSMD8, a base containing 6 ATPases and
CC       few additional components. Interacts with DDI2.
CC       {ECO:0000256|ARBA:ARBA00096404}.
CC   -!- SIMILARITY: Belongs to the proteasome subunit S14 family.
CC       {ECO:0000256|ARBA:ARBA00009627}.
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DR   EMBL; AK002921; BAB22458.2; -; mRNA.
DR   EMBL; AK003436; BAB22789.2; -; mRNA.
DR   EMBL; AK027973; BAC25683.1; -; mRNA.
DR   EMBL; AK150493; BAE29609.1; -; mRNA.
DR   EMBL; AK150982; BAE30009.1; -; mRNA.
DR   EMBL; AK159216; BAE34905.1; -; mRNA.
DR   EMBL; AK166718; BAE38968.1; -; mRNA.
DR   RefSeq; NP_080821.3; NM_026545.3.
DR   AlphaFoldDB; Q9CPS5; -.
DR   SMR; Q9CPS5; -.
DR   jPOST; Q9CPS5; -.
DR   ProteomicsDB; 334820; -.
DR   Antibodypedia; 1714; 161 antibodies from 28 providers.
DR   DNASU; 57296; -.
DR   GeneID; 57296; -.
DR   KEGG; mmu:57296; -.
DR   AGR; MGI:1888669; -.
DR   CTD; 5714; -.
DR   MGI; MGI:1888669; Psmd8.
DR   VEuPathDB; HostDB:ENSMUSG00000030591; -.
DR   GeneTree; ENSGT00390000014682; -.
DR   HOGENOM; CLU_046003_2_0_1; -.
DR   OMA; HIMDGYF; -.
DR   OrthoDB; 409122at2759; -.
DR   BioGRID-ORCS; 57296; 26 hits in 78 CRISPR screens.
DR   Bgee; ENSMUSG00000030591; Expressed in placenta labyrinth and 267 other cell types or tissues.
DR   GO; GO:0005838; C:proteasome regulatory particle; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   FunFam; 1.25.40.990:FF:000001; 26S proteasome non-ATPase regulatory subunit; 1.
DR   Gene3D; 1.25.40.990; -; 1.
DR   InterPro; IPR006746; 26S_Psome_Rpn12.
DR   InterPro; IPR033464; CSN8_PSD8_EIF3K.
DR   InterPro; IPR000717; PCI_dom.
DR   PANTHER; PTHR12387; 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8; 1.
DR   PANTHER; PTHR12387:SF0; 26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8; 1.
DR   Pfam; PF10075; CSN8_PSD8_EIF3K; 1.
DR   PROSITE; PS50250; PCI; 1.
PE   1: Evidence at protein level;
KW   Proteasome {ECO:0000256|ARBA:ARBA00022942}.
FT   DOMAIN          101..270
FT                   /note="PCI"
FT                   /evidence="ECO:0000259|PROSITE:PS50250"
SQ   SEQUENCE   289 AA;  32769 MW;  2D61CFE344475C55 CRC64;
     MAATAATVNG STTVSSSGPA ATSVGILQAA AGMYEQLKDE WNRKNPNLSK CGEELGRLKL
     VLLELNFLPT TGTKLTKQQL ILARDILEIG AQWSILCKDI PSFERYMAQL KCYYFDYKEQ
     LPESAYMHQL LGLNLLFLLS QNRVAEFHTE LERLPAKDIQ TNVYIKHPVS LEQYLMEGSY
     NKVFLAKGNI PAESYTFFID ILLDTIRDEI AGCIEKAYEK ILFAEATRIL FFSTPKKMTD
     YAKKRGWVLG PNNYYSFASQ QQKPEDSTIP STELAKQVIE YARQLEMIV
//