ID   KAO1_HORVU              Reviewed;         499 AA.
AC   Q9AXH9;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   02-SEP-2026, entry version 111.
DE   RecName: Full=Ent-kaurenoic acid oxidase 1 {ECO:0000303|PubMed:11172076};
DE            EC=1.14.14.107 {ECO:0000269|PubMed:11172076};
DE   AltName: Full=gpr5 {ECO:0000303|PubMed:11172076};
GN   Name=KAO1 {ECO:0000303|PubMed:11172076};
GN   Synonyms=GPR5 {ECO:0000303|PubMed:11172076};
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF
RP   ASN-372, AND PATHWAY.
RC   STRAIN=cv. Himalaya;
RX   PubMed=11172076; DOI=10.1073/pnas.98.4.2065;
RA   Helliwell C.A., Chandler P.M., Poole A., Dennis E.S., Peacock J.W.;
RT   "The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three
RT   steps of the gibberellin biosynthesis pathway.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:2065-2070(2001).
CC   -!- FUNCTION: Catalyzes three successive oxidations of ent-kaurenoic acid
CC       giving gibberellin 12 (GA12), a key step in gibberellins (GAs)
CC       biosynthesis. GAs, which are involved many processes, including stem
CC       elongation, play a central role in plant development.
CC       {ECO:0000269|PubMed:11172076}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ent-kaur-16-en-19-oate + 3 reduced [NADPH--hemoprotein
CC         reductase] + 3 O2 = gibberellin A12 + 3 oxidized [NADPH--hemoprotein
CC         reductase] + 4 H2O + 4 H(+); Xref=Rhea:RHEA:33219, Rhea:RHEA-
CC         COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57297,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:58627;
CC         EC=1.14.14.107; Evidence={ECO:0000269|PubMed:11172076};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33220;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ent-kaur-16-en-19-oate + reduced [NADPH--hemoprotein
CC         reductase] + O2 = ent-7alpha-hydroxykaur-16-en-19-oate + oxidized
CC         [NADPH--hemoprotein reductase] + H2O + H(+); Xref=Rhea:RHEA:19241,
CC         Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57297,
CC         ChEBI:CHEBI:57298, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19242;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ent-7alpha-hydroxykaur-16-en-19-oate + reduced
CC         [NADPH--hemoprotein reductase] + O2 = gibberellin A12 aldehyde +
CC         oxidized [NADPH--hemoprotein reductase] + 2 H2O + H(+);
CC         Xref=Rhea:RHEA:22904, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:57298, ChEBI:CHEBI:57432, ChEBI:CHEBI:57618,
CC         ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:11172076};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22905;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=gibberellin A12 aldehyde + reduced [NADPH--hemoprotein
CC         reductase] + O2 = gibberellin A12 + oxidized [NADPH--hemoprotein
CC         reductase] + H2O + 2 H(+); Xref=Rhea:RHEA:22700, Rhea:RHEA-
CC         COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57432,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:58627;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22701;
CC         Evidence={ECO:0000269|PubMed:11172076};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC         Evidence={ECO:0000250|UniProtKB:P04798};
CC   -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC       {ECO:0000269|PubMed:11172076}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:O23051}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; AF326277; AAK11616.1; -; mRNA.
DR   AlphaFoldDB; Q9AXH9; -.
DR   SMR; Q9AXH9; -.
DR   EnsemblPlants; HORVU.AIZU_6.PROJ.7HG00414530.1; cds:HORVU.AIZU_6.PROJ.7HG00414530.1; HORVU.AIZU_6.PROJ.7HG00414530.
DR   EnsemblPlants; HORVU.B1K-04-12.PROJ.7HG00578710.1; cds:HORVU.B1K-04-12.PROJ.7HG00578710.1; HORVU.B1K-04-12.PROJ.7HG00578710.
DR   EnsemblPlants; HORVU.BARKE.PROJ.7HG00578110.1; cds:HORVU.BARKE.PROJ.7HG00578110.1; HORVU.BARKE.PROJ.7HG00578110.
DR   EnsemblPlants; HORVU.BONUS.PROJ.7HG00419880.1; cds:HORVU.BONUS.PROJ.7HG00419880.1; HORVU.BONUS.PROJ.7HG00419880.
DR   EnsemblPlants; HORVU.BOWMAN.PROJ.7HG00419440.1; cds:HORVU.BOWMAN.PROJ.7HG00419440.1; HORVU.BOWMAN.PROJ.7HG00419440.
DR   EnsemblPlants; HORVU.FOMA.PROJ.7HG00419900.1; cds:HORVU.FOMA.PROJ.7HG00419900.1; HORVU.FOMA.PROJ.7HG00419900.
DR   EnsemblPlants; HORVU.GOLDEN_MELON.PROJ.7HG00421710.1; cds:HORVU.GOLDEN_MELON.PROJ.7HG00421710.1; HORVU.GOLDEN_MELON.PROJ.7HG00421710.
DR   EnsemblPlants; HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.1; cds:HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.1; HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.
DR   EnsemblPlants; HORVU.HID112XXI.PROJ.7HG00402460.1; cds:HORVU.HID112XXI.PROJ.7HG00402460.1; HORVU.HID112XXI.PROJ.7HG00402460.
DR   EnsemblPlants; HORVU.HID249.PROJ.7HG00402640.1; cds:HORVU.HID249.PROJ.7HG00402640.1; HORVU.HID249.PROJ.7HG00402640.
DR   EnsemblPlants; HORVU.HID251.PROJ.7HG00402840.1; cds:HORVU.HID251.PROJ.7HG00402840.1; HORVU.HID251.PROJ.7HG00402840.
DR   EnsemblPlants; HORVU.HID380.PROJ.7HG00414870.1; cds:HORVU.HID380.PROJ.7HG00414870.1; HORVU.HID380.PROJ.7HG00414870.
DR   EnsemblPlants; HORVU.HID84.PROJ.7HG00406350.1; cds:HORVU.HID84.PROJ.7HG00406350.1; HORVU.HID84.PROJ.7HG00406350.
DR   EnsemblPlants; HORVU.HOCKETT.PROJ.7HG00567160.1; cds:HORVU.HOCKETT.PROJ.7HG00567160.1; HORVU.HOCKETT.PROJ.7HG00567160.
DR   EnsemblPlants; HORVU.HOR_10892.PROJ.7HG00418550.1; cds:HORVU.HOR_10892.PROJ.7HG00418550.1; HORVU.HOR_10892.PROJ.7HG00418550.
DR   EnsemblPlants; HORVU.HOR_1168.PROJ.7HG00416520.1; cds:HORVU.HOR_1168.PROJ.7HG00416520.1; HORVU.HOR_1168.PROJ.7HG00416520.
DR   EnsemblPlants; HORVU.HOR_12184.PROJ.7HG00412780.1; cds:HORVU.HOR_12184.PROJ.7HG00412780.1; HORVU.HOR_12184.PROJ.7HG00412780.
DR   EnsemblPlants; HORVU.HOR_13663.PROJ.7HG00416690.1; cds:HORVU.HOR_13663.PROJ.7HG00416690.1; HORVU.HOR_13663.PROJ.7HG00416690.
DR   EnsemblPlants; HORVU.HOR_13821.PROJ.7HG00567570.1; cds:HORVU.HOR_13821.PROJ.7HG00567570.1; HORVU.HOR_13821.PROJ.7HG00567570.
DR   EnsemblPlants; HORVU.HOR_14061.PROJ.7HG00417190.1; cds:HORVU.HOR_14061.PROJ.7HG00417190.1; HORVU.HOR_14061.PROJ.7HG00417190.
DR   EnsemblPlants; HORVU.HOR_1702.PROJ.7HG00417980.1; cds:HORVU.HOR_1702.PROJ.7HG00417980.1; HORVU.HOR_1702.PROJ.7HG00417980.
DR   EnsemblPlants; HORVU.HOR_18321.PROJ.7HG00416960.1; cds:HORVU.HOR_18321.PROJ.7HG00416960.1; HORVU.HOR_18321.PROJ.7HG00416960.
DR   EnsemblPlants; HORVU.HOR_19184.PROJ.7HG00415150.1; cds:HORVU.HOR_19184.PROJ.7HG00415150.1; HORVU.HOR_19184.PROJ.7HG00415150.
DR   EnsemblPlants; HORVU.HOR_21322.PROJ.7HG00415220.1; cds:HORVU.HOR_21322.PROJ.7HG00415220.1; HORVU.HOR_21322.PROJ.7HG00415220.
DR   EnsemblPlants; HORVU.HOR_21595.PROJ.7HG00411540.1; cds:HORVU.HOR_21595.PROJ.7HG00411540.1; HORVU.HOR_21595.PROJ.7HG00411540.
DR   EnsemblPlants; HORVU.HOR_2180.PROJ.7HG00405110.1; cds:HORVU.HOR_2180.PROJ.7HG00405110.1; HORVU.HOR_2180.PROJ.7HG00405110.
DR   EnsemblPlants; HORVU.HOR_2830.PROJ.7HG00412670.1; cds:HORVU.HOR_2830.PROJ.7HG00412670.1; HORVU.HOR_2830.PROJ.7HG00412670.
DR   EnsemblPlants; HORVU.HOR_3081.PROJ.7HG00563480.1; cds:HORVU.HOR_3081.PROJ.7HG00563480.1; HORVU.HOR_3081.PROJ.7HG00563480.
DR   EnsemblPlants; HORVU.HOR_3365.PROJ.7HG00561300.1; cds:HORVU.HOR_3365.PROJ.7HG00561300.1; HORVU.HOR_3365.PROJ.7HG00561300.
DR   EnsemblPlants; HORVU.HOR_4224.PROJ.7HG00415110.1; cds:HORVU.HOR_4224.PROJ.7HG00415110.1; HORVU.HOR_4224.PROJ.7HG00415110.
DR   EnsemblPlants; HORVU.HOR_495.PROJ.7HG00412270.1; cds:HORVU.HOR_495.PROJ.7HG00412270.1; HORVU.HOR_495.PROJ.7HG00412270.
DR   EnsemblPlants; HORVU.HOR_7172.PROJ.7HG00399950.1; cds:HORVU.HOR_7172.PROJ.7HG00399950.1; HORVU.HOR_7172.PROJ.7HG00399950.
DR   EnsemblPlants; HORVU.HOR_7385.PROJ.7HG00416340.1; cds:HORVU.HOR_7385.PROJ.7HG00416340.1; HORVU.HOR_7385.PROJ.7HG00416340.
DR   EnsemblPlants; HORVU.HOR_8148.PROJ.7HG00570370.1; cds:HORVU.HOR_8148.PROJ.7HG00570370.1; HORVU.HOR_8148.PROJ.7HG00570370.
DR   EnsemblPlants; HORVU.HOR_9972.PROJ.7HG00414350.1; cds:HORVU.HOR_9972.PROJ.7HG00414350.1; HORVU.HOR_9972.PROJ.7HG00414350.
DR   EnsemblPlants; HORVU.MAXIMUS.PROJ.7HG00418190.1; cds:HORVU.MAXIMUS.PROJ.7HG00418190.1; HORVU.MAXIMUS.PROJ.7HG00418190.
DR   EnsemblPlants; HORVU.OUN333.PROJ.7HG00572580.1; cds:HORVU.OUN333.PROJ.7HG00572580.1; HORVU.OUN333.PROJ.7HG00572580.
DR   EnsemblPlants; HORVU.WBDC078.PROJ.7HG00403930.1; cds:HORVU.WBDC078.PROJ.7HG00403930.1; HORVU.WBDC078.PROJ.7HG00403930.
DR   EnsemblPlants; HORVU.WBDC103.PROJ.7HG00400580.1; cds:HORVU.WBDC103.PROJ.7HG00400580.1; HORVU.WBDC103.PROJ.7HG00400580.
DR   EnsemblPlants; HORVU.WBDC199.PROJ.7HG00404380.1; cds:HORVU.WBDC199.PROJ.7HG00404380.1; HORVU.WBDC199.PROJ.7HG00404380.
DR   EnsemblPlants; HORVU.WBDC207.PROJ.7HG00405500.1; cds:HORVU.WBDC207.PROJ.7HG00405500.1; HORVU.WBDC207.PROJ.7HG00405500.
DR   EnsemblPlants; HORVU.ZDM01467.PROJ.7HG00568410.1; cds:HORVU.ZDM01467.PROJ.7HG00568410.1; HORVU.ZDM01467.PROJ.7HG00568410.
DR   Gramene; HORVU.AIZU_6.PROJ.7HG00414530.1; cds:HORVU.AIZU_6.PROJ.7HG00414530.1; HORVU.AIZU_6.PROJ.7HG00414530.
DR   Gramene; HORVU.B1K-04-12.PROJ.7HG00578710.1; cds:HORVU.B1K-04-12.PROJ.7HG00578710.1; HORVU.B1K-04-12.PROJ.7HG00578710.
DR   Gramene; HORVU.BARKE.PROJ.7HG00578110.1; cds:HORVU.BARKE.PROJ.7HG00578110.1; HORVU.BARKE.PROJ.7HG00578110.
DR   Gramene; HORVU.BONUS.PROJ.7HG00419880.1; cds:HORVU.BONUS.PROJ.7HG00419880.1; HORVU.BONUS.PROJ.7HG00419880.
DR   Gramene; HORVU.BOWMAN.PROJ.7HG00419440.1; cds:HORVU.BOWMAN.PROJ.7HG00419440.1; HORVU.BOWMAN.PROJ.7HG00419440.
DR   Gramene; HORVU.FOMA.PROJ.7HG00419900.1; cds:HORVU.FOMA.PROJ.7HG00419900.1; HORVU.FOMA.PROJ.7HG00419900.
DR   Gramene; HORVU.GOLDEN_MELON.PROJ.7HG00421710.1; cds:HORVU.GOLDEN_MELON.PROJ.7HG00421710.1; HORVU.GOLDEN_MELON.PROJ.7HG00421710.
DR   Gramene; HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.1; cds:HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.1; HORVU.GOLDEN_PROMISE.PROJ.7HG00570210.
DR   Gramene; HORVU.HID112XXI.PROJ.7HG00402460.1; cds:HORVU.HID112XXI.PROJ.7HG00402460.1; HORVU.HID112XXI.PROJ.7HG00402460.
DR   Gramene; HORVU.HID249.PROJ.7HG00402640.1; cds:HORVU.HID249.PROJ.7HG00402640.1; HORVU.HID249.PROJ.7HG00402640.
DR   Gramene; HORVU.HID251.PROJ.7HG00402840.1; cds:HORVU.HID251.PROJ.7HG00402840.1; HORVU.HID251.PROJ.7HG00402840.
DR   Gramene; HORVU.HID380.PROJ.7HG00414870.1; cds:HORVU.HID380.PROJ.7HG00414870.1; HORVU.HID380.PROJ.7HG00414870.
DR   Gramene; HORVU.HID84.PROJ.7HG00406350.1; cds:HORVU.HID84.PROJ.7HG00406350.1; HORVU.HID84.PROJ.7HG00406350.
DR   Gramene; HORVU.HOCKETT.PROJ.7HG00567160.1; cds:HORVU.HOCKETT.PROJ.7HG00567160.1; HORVU.HOCKETT.PROJ.7HG00567160.
DR   Gramene; HORVU.HOR_10892.PROJ.7HG00418550.1; cds:HORVU.HOR_10892.PROJ.7HG00418550.1; HORVU.HOR_10892.PROJ.7HG00418550.
DR   Gramene; HORVU.HOR_1168.PROJ.7HG00416520.1; cds:HORVU.HOR_1168.PROJ.7HG00416520.1; HORVU.HOR_1168.PROJ.7HG00416520.
DR   Gramene; HORVU.HOR_12184.PROJ.7HG00412780.1; cds:HORVU.HOR_12184.PROJ.7HG00412780.1; HORVU.HOR_12184.PROJ.7HG00412780.
DR   Gramene; HORVU.HOR_13663.PROJ.7HG00416690.1; cds:HORVU.HOR_13663.PROJ.7HG00416690.1; HORVU.HOR_13663.PROJ.7HG00416690.
DR   Gramene; HORVU.HOR_13821.PROJ.7HG00567570.1; cds:HORVU.HOR_13821.PROJ.7HG00567570.1; HORVU.HOR_13821.PROJ.7HG00567570.
DR   Gramene; HORVU.HOR_14061.PROJ.7HG00417190.1; cds:HORVU.HOR_14061.PROJ.7HG00417190.1; HORVU.HOR_14061.PROJ.7HG00417190.
DR   Gramene; HORVU.HOR_1702.PROJ.7HG00417980.1; cds:HORVU.HOR_1702.PROJ.7HG00417980.1; HORVU.HOR_1702.PROJ.7HG00417980.
DR   Gramene; HORVU.HOR_18321.PROJ.7HG00416960.1; cds:HORVU.HOR_18321.PROJ.7HG00416960.1; HORVU.HOR_18321.PROJ.7HG00416960.
DR   Gramene; HORVU.HOR_19184.PROJ.7HG00415150.1; cds:HORVU.HOR_19184.PROJ.7HG00415150.1; HORVU.HOR_19184.PROJ.7HG00415150.
DR   Gramene; HORVU.HOR_21322.PROJ.7HG00415220.1; cds:HORVU.HOR_21322.PROJ.7HG00415220.1; HORVU.HOR_21322.PROJ.7HG00415220.
DR   Gramene; HORVU.HOR_21595.PROJ.7HG00411540.1; cds:HORVU.HOR_21595.PROJ.7HG00411540.1; HORVU.HOR_21595.PROJ.7HG00411540.
DR   Gramene; HORVU.HOR_2180.PROJ.7HG00405110.1; cds:HORVU.HOR_2180.PROJ.7HG00405110.1; HORVU.HOR_2180.PROJ.7HG00405110.
DR   Gramene; HORVU.HOR_2830.PROJ.7HG00412670.1; cds:HORVU.HOR_2830.PROJ.7HG00412670.1; HORVU.HOR_2830.PROJ.7HG00412670.
DR   Gramene; HORVU.HOR_3081.PROJ.7HG00563480.1; cds:HORVU.HOR_3081.PROJ.7HG00563480.1; HORVU.HOR_3081.PROJ.7HG00563480.
DR   Gramene; HORVU.HOR_3365.PROJ.7HG00561300.1; cds:HORVU.HOR_3365.PROJ.7HG00561300.1; HORVU.HOR_3365.PROJ.7HG00561300.
DR   Gramene; HORVU.HOR_4224.PROJ.7HG00415110.1; cds:HORVU.HOR_4224.PROJ.7HG00415110.1; HORVU.HOR_4224.PROJ.7HG00415110.
DR   Gramene; HORVU.HOR_495.PROJ.7HG00412270.1; cds:HORVU.HOR_495.PROJ.7HG00412270.1; HORVU.HOR_495.PROJ.7HG00412270.
DR   Gramene; HORVU.HOR_7172.PROJ.7HG00399950.1; cds:HORVU.HOR_7172.PROJ.7HG00399950.1; HORVU.HOR_7172.PROJ.7HG00399950.
DR   Gramene; HORVU.HOR_7385.PROJ.7HG00416340.1; cds:HORVU.HOR_7385.PROJ.7HG00416340.1; HORVU.HOR_7385.PROJ.7HG00416340.
DR   Gramene; HORVU.HOR_8148.PROJ.7HG00570370.1; cds:HORVU.HOR_8148.PROJ.7HG00570370.1; HORVU.HOR_8148.PROJ.7HG00570370.
DR   Gramene; HORVU.HOR_9972.PROJ.7HG00414350.1; cds:HORVU.HOR_9972.PROJ.7HG00414350.1; HORVU.HOR_9972.PROJ.7HG00414350.
DR   Gramene; HORVU.MAXIMUS.PROJ.7HG00418190.1; cds:HORVU.MAXIMUS.PROJ.7HG00418190.1; HORVU.MAXIMUS.PROJ.7HG00418190.
DR   Gramene; HORVU.OUN333.PROJ.7HG00572580.1; cds:HORVU.OUN333.PROJ.7HG00572580.1; HORVU.OUN333.PROJ.7HG00572580.
DR   Gramene; HORVU.WBDC078.PROJ.7HG00403930.1; cds:HORVU.WBDC078.PROJ.7HG00403930.1; HORVU.WBDC078.PROJ.7HG00403930.
DR   Gramene; HORVU.WBDC103.PROJ.7HG00400580.1; cds:HORVU.WBDC103.PROJ.7HG00400580.1; HORVU.WBDC103.PROJ.7HG00400580.
DR   Gramene; HORVU.WBDC199.PROJ.7HG00404380.1; cds:HORVU.WBDC199.PROJ.7HG00404380.1; HORVU.WBDC199.PROJ.7HG00404380.
DR   Gramene; HORVU.WBDC207.PROJ.7HG00405500.1; cds:HORVU.WBDC207.PROJ.7HG00405500.1; HORVU.WBDC207.PROJ.7HG00405500.
DR   Gramene; HORVU.ZDM01467.PROJ.7HG00568410.1; cds:HORVU.ZDM01467.PROJ.7HG00568410.1; HORVU.ZDM01467.PROJ.7HG00568410.
DR   OMA; KLWEVYM; -.
DR   BRENDA; 1.14.14.107; 2687.
DR   UniPathway; UPA00390; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0051777; F:ent-kaurenoic acid monooxygenase activity; EXP:UniProtKB.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016132; P:brassinosteroid biosynthetic process; IEA:TreeGrafter.
DR   GO; GO:0010268; P:brassinosteroid homeostasis; IEA:TreeGrafter.
DR   GO; GO:0009686; P:gibberellin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016125; P:sterol metabolic process; IEA:TreeGrafter.
DR   CDD; cd11043; CYP90-like; 1.
DR   FunFam; 1.10.630.10:FF:000052; Ent-kaurenoic acid oxidase; 1.
DR   Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   PANTHER; PTHR24286; CYTOCHROME P450 26; 1.
DR   PANTHER; PTHR24286:SF356; ENT-KAURENOIC ACID OXIDASE 2; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; Cytochrome P450; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..499
FT                   /note="Ent-kaurenoic acid oxidase 1"
FT                   /id="PRO_0000052180"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         443
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   MUTAGEN         372
FT                   /note="N->K: In M594; induces dwarfism, due to accumulation
FT                   of kaurenoic acid."
FT                   /evidence="ECO:0000269|PubMed:11172076"
SQ   SEQUENCE   499 AA;  55876 MW;  76CB0C5CC6C3641F CRC64;
     MGEGAWWAVA AVVAALAVVA LDAAVRAAHA WYWTASLGAG RRGRLPPGDM GWPLVGGMWA
     FLRAFKSGRP DSFIDSFARR FGRAGLYRAF MFSSPTIMAT TPEACKQVLM DDDAFVTGWP
     KATVALIGPK SFVNMGYDEH RRLRKLTAAP INGFDALTSY LGFIDDTVVT TLRGWSERGG
     DGHFEFLTEL RRMTFRIIVQ IFMGGADERT AAELERTYTE LNYGMRAMAI DLPGFAYHKA
     IRARRRLVAA LQRVLDERRA RGGKTAAGAA APVDMMDRLI AVEDEGGRRL QDDEIIDVLV
     MYLNAGHESS GHITMWATVF LQENPEILAK AKAEQEAIMR SIPPGQKGLT LRDFRKMAYL
     SQVVDETLRF VNISFVSFRQ ATRDVFVNGY LIPKGWKVQL WYRSVHMDPQ VYPDPKKFDP
     SRWEGPPPRA GTFLPFGLGT RLCPGNDLAK LEISVFLHHF LLGYKLTRKN PNCRVRYLPH
     PRPVDNCLAK ITRLSSSHG
//