ID   Q9AIV2_GEOSE            Unreviewed;       588 AA.
AC   Q9AIV2;
DT   01-JUN-2001, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2001, sequence version 1.
DT   02-SEP-2026, entry version 88.
DE   SubName: Full=Neopullulanase {ECO:0000313|EMBL:AAK15003.1};
GN   Name=npl {ECO:0000313|EMBL:AAK15003.1};
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Anoxybacillaceae;
OC   Geobacillus.
OX   NCBI_TaxID=1422 {ECO:0000313|EMBL:AAK15003.1};
RN   [1] {ECO:0000313|EMBL:AAK15003.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=IMA6503 {ECO:0000313|EMBL:AAK15003.1};
RX   PubMed=11834127; DOI=10.1042/BA20010052;
RA   Cheong K.A., Kim T.J., Yoon J.W., Park C.S., Lee T.S., Kim Y.B., Park K.H.,
RA   Kim J.W.;
RT   "Catalytic activities of intracellular dimeric neopullulanase on
RT   cyclodextrin, acarbose and maltose.";
RL   Biotechnol. Appl. Biochem. 35:27-34(2002).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
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DR   EMBL; AF233372; AAK15003.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9AIV2; -.
DR   SMR; Q9AIV2; -.
DR   CAZy; CBM34; Carbohydrate-Binding Module Family 34.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   BRENDA; 3.2.1.135; 623.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11338; AmyAc_CMD; 1.
DR   CDD; cd02857; E_set_CDase_PDE_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   Gene3D; 3.90.400.10; Oligo-1,6-glucosidase, Domain 2; 1.
DR   InterPro; IPR006047; GH13_cat_dom.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR004185; Glyco_hydro_13_lg-like_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032091; Malt_amylase-like_C.
DR   InterPro; IPR045857; O16G_dom_2.
DR   PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR   PANTHER; PTHR10357:SF210; MALTODEXTRIN GLUCOSIDASE; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02903; Alpha-amylase_N; 1.
DR   Pfam; PF16657; Malt_amylase_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801}.
FT   DOMAIN          139..497
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   588 AA;  68930 MW;  5A422411B1D78DC2 CRC64;
     MRKEAIYHRP ADNFAYAYDS ETLHLRLRTK KDDIDRVELL HGDPYDWQNG AWQFQTMPMR
     KTGSDELFDY WFAEVKPPYR RLRYGFVLYS GEEKLVYTEK GFYFEAPIDD TAYYFCFPFL
     HRVDLFEAPD WVKDTVWYQI FPERFANGNP SISPEGSRPW GNEDPTPTSF FGGDLQGIID
     HLDYLVDLGI TGIYLTPIFR SPSNHKYDTA DYFEVDPHFG DKETLKTLID RCHEKGIRVM
     LDAVFNHCGY EFAPFQDVWK NGESSKYKDW FHIHEFPLQT ESRPNYDTFA FVPQMPKLNT
     ANPEVKRYLL DVATYWIREF DIDGWRLDVA NEIDHEFWRE FRQAVKALKP DVYILGEIWH
     DAMPWLRGDQ FDAVMNYPFT DGVLRFFAKE EIGARQFANQ MVHVLHSYPN NVNEAAFNLL
     GSHDTSRILT VCGGDIRKVK LLFLFQLTFT GSPCIYYGDE IGMTGGNDPE CRKCMVWDPM
     QQNKELHQHV KQLIALRKQY RSLRRGEISF LHADDEMNYL IYKKTDGDET VLVIINRSDQ
     KADIPIPLDA RGTWLVNLLT GERFAAEAET LCTSLPPYGF VLYAIERW
//