ID LACG1_STRPN Reviewed; 470 AA. AC Q97SA9; DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2001, sequence version 1. DT 02-SEP-2026, entry version 131. DE RecName: Full=6-phospho-beta-galactosidase 1 {ECO:0000255|HAMAP-Rule:MF_01574}; DE EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574}; DE AltName: Full=Beta-D-phosphogalactoside galactohydrolase 1 {ECO:0000255|HAMAP-Rule:MF_01574}; DE Short=PGALase 1 {ECO:0000255|HAMAP-Rule:MF_01574}; DE AltName: Full=P-beta-Gal 1 {ECO:0000255|HAMAP-Rule:MF_01574}; DE Short=PBG 1 {ECO:0000255|HAMAP-Rule:MF_01574}; GN Name=lacG1 {ECO:0000255|HAMAP-Rule:MF_01574}; Synonyms=lacG-1; GN OrderedLocusNames=SP_0477; OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4). OC Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=170187; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-334 / TIGR4; RX PubMed=11463916; DOI=10.1126/science.1061217; RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D., RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., RA Morrison D.A., Hollingshead S.K., Fraser C.M.; RT "Complete genome sequence of a virulent isolate of Streptococcus RT pneumoniae."; RL Science 293:498-506(2001). CC -!- CATALYTIC ACTIVITY: CC Reaction=a 6-phospho-beta-D-galactoside + H2O = D-galactose 6-phosphate CC + an alcohol; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01574}; CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6- CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. CC {ECO:0000255|HAMAP-Rule:MF_01574}. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family. CC {ECO:0000255|HAMAP-Rule:MF_01574}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE005672; AAK74636.1; -; Genomic_DNA. DR PIR; C95055; C95055. DR RefSeq; WP_000429431.1; NC_003028.3. DR AlphaFoldDB; Q97SA9; -. DR SMR; Q97SA9; -. DR EnsemblBacteria; AAK74636; AAK74636; SP_0477. DR KEGG; spn:SP_0477; -. DR eggNOG; COG2723; Bacteria. DR PhylomeDB; Q97SA9; -. DR BioCyc; SPNE170187:G1FZB-494-MONOMER; -. DR UniPathway; UPA00542; UER00605. DR Proteomes; UP000000585; Chromosome. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0008422; F:beta-glucosidase activity; IEA:TreeGrafter. DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro. DR FunFam; 3.20.20.80:FF:000004; Beta-glucosidase 6-phospho-beta-glucosidase; 1. DR Gene3D; 3.20.20.80; Glycosidases; 1. DR HAMAP; MF_01574; LacG; 1. DR InterPro; IPR005928; 6P-beta-galactosidase. DR InterPro; IPR017853; GH_hydrolase_sf. DR InterPro; IPR001360; Glyco_hydro_1. DR NCBIfam; TIGR01233; lacG; 1. DR NCBIfam; NF010036; PRK13511.1; 1. DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1. DR PANTHER; PTHR10353:SF36; LP05116P; 1. DR Pfam; PF00232; Glyco_hydro_1; 1. DR PRINTS; PR00131; GLHYDRLASE1. DR SUPFAM; SSF51445; (Trans)glycosidases; 1. PE 3: Inferred from homology; KW Glycosidase; Hydrolase; Reference proteome. FT CHAIN 1..470 FT /note="6-phospho-beta-galactosidase 1" FT /id="PRO_0000260730" FT ACT_SITE 164 FT /note="Proton donor" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT ACT_SITE 378 FT /note="Nucleophile" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 23 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 120 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 163 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 164 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 300 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 434 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 435 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 441 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" FT BINDING 443 FT /ligand="D-galactose 6-phosphate" FT /ligand_id="ChEBI:CHEBI:91004" FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574" SQ SEQUENCE 470 AA; 54633 MW; 964D7022454360C6 CRC64; MENLQVKALP KEFLLGTATA AYQVEGATRV DGKGINMWDV YLQENSPFLP DPASDFYYRY EEDIALAAEH GLQALRLSIS WVRIFPDIDG DANVLAVHYY HRVFQSCLKH NVIPFVSLHH FDSPQKMLET GDWLNRENID RFIRYARFCF QEFTEVKHWF TINELMSLAA GQYIGGQFPP NHHFQLSEAI QANHNMLLAH ALAVLEFHQL GIEGKVGCIH ALKPGYPIDG QKENILAAKR YDVYNNKFLL DGTFLGYYSE DTLFHLNQIL EANNSSFIIE DGDLEIMKRA APLNTMFGMN YYRSEFIREY KGENRQEFNS TGIKGQSSFK LNALGEFVKK PGIPTTDWDW NIYPQGLFDM LLRIKEEYPQ HPVIYLTENG TALKEVKPEG ENDIIDDSKR IRYIEQHLHK VLEARDRGVN IQGYFIWSLQ DQFSWANGYN KRYGLFFVDY ETQKRYIKKS ALWVKGLKRN //