ID   Q97LL8_CLOAB            Unreviewed;       532 AA.
AC   Q97LL8;
DT   01-OCT-2001, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2001, sequence version 1.
DT   02-SEP-2026, entry version 109.
DE   SubName: Full=Beta-mannanase ManB-like enzyme, contains ChW-repeats {ECO:0000313|EMBL:AAK78519.1};
GN   OrderedLocusNames=CA_C0540 {ECO:0000313|EMBL:AAK78519.1};
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM
OS   19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W).
OC   Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562 {ECO:0000313|EMBL:AAK78519.1, ECO:0000313|Proteomes:UP000000814};
RN   [1] {ECO:0000313|EMBL:AAK78519.1, ECO:0000313|Proteomes:UP000000814}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787
RC   {ECO:0000313|Proteomes:UP000000814};
RX   PubMed=11466286; DOI=10.1128/JB.183.16.4823-4838.2001;
RA   Nolling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 26 family.
CC       {ECO:0000256|ARBA:ARBA00007754, ECO:0000256|PROSITE-ProRule:PRU01100}.
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DR   EMBL; AE001437; AAK78519.1; -; Genomic_DNA.
DR   PIR; D96966; D96966.
DR   RefSeq; WP_010963861.1; NC_003030.1.
DR   AlphaFoldDB; Q97LL8; -.
DR   STRING; 272562.CA_C0540; -.
DR   DNASU; 1116723; -.
DR   GeneID; 44997050; -.
DR   KEGG; cac:CA_C0540; -.
DR   PATRIC; fig|272562.8.peg.743; -.
DR   eggNOG; COG4124; Bacteria.
DR   HOGENOM; CLU_041638_0_0_9; -.
DR   OrthoDB; 9806342at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:InterPro.
DR   GO; GO:0006080; P:substituted mannan metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR006637; ChW.
DR   InterPro; IPR022790; GH26_dom.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR000805; Glyco_hydro_26.
DR   PANTHER; PTHR40079:SF4; GH26 DOMAIN-CONTAINING PROTEIN-RELATED; 1.
DR   PANTHER; PTHR40079; MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED; 1.
DR   Pfam; PF07538; ChW; 3.
DR   Pfam; PF02156; Glyco_hydro_26; 1.
DR   PRINTS; PR00739; GLHYDRLASE26.
DR   SMART; SM00728; ChW; 3.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS51764; GH26; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU01100};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01100}; Reference proteome {ECO:0000313|Proteomes:UP000000814};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           27..532
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5038696094"
FT   DOMAIN          44..380
FT                   /note="GH26"
FT                   /evidence="ECO:0000259|PROSITE:PS51764"
FT   REGION          76..95
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        204
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01100"
FT   ACT_SITE        307
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01100"
SQ   SEQUENCE   532 AA;  59053 MW;  8FD047F75AA06A84 CRC64;
     MILNKRKKVL ISMVTMVGIL SPLANSKVYA IPTTCNSGIF VGRAENLDAI DIMKDPNYTG
     KVSIVGFDVA NCSDNNSDDN HKNHNETAIG NGPTPKWHNS NYTSDFLNVA QQGKDTMISA
     TLPNPVPSQE IGSVGQIAYG SQISDADFKE LASVDIKNIN QSSPYFVRNY GQMVSKLGDI
     IEKYHTELMD SNGNATIYLR PFHEMNSPWL WWGFKNQDDF KNLWIHLHNY LVNDRKFTWL
     KFCFAASAGD PSLDPAHVKG ANSYYPGNNY VDTVALDGYS DDPSNDSGIK KQYAELTATG
     KPFGFAELGA KVDGDFVNVP NERTQQNVET RPFNYQKWSD AIQKYYSNAK YCIVFGGAYD
     PRNNLHGTSL FSNSQEPNTS INYQAHVQNI GWQDSVQDGD AAGTIEKALR MEAIKINYPN
     NAGLHVEYSA QVQNKGWMPA VRDGAIAGTV QEGLRMEAIK ISLKDSLGNV SNDYSVYYRA
     QVQNKGWMPW VKNGAIAGTV QEGLRMEAIE IYVVKNNTPV ANFMDNKVAN AQ
//