ID RMC1_HUMAN Reviewed; 657 AA. AC Q96DM3; Q9BU17; Q9Y5M0; DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot. DT 24-MAY-2004, sequence version 2. DT 04-NOV-2026, entry version 164. DE RecName: Full=Regulator of MON1-CCZ1 complex {ECO:0000303|PubMed:29038162}; DE AltName: Full=Colon cancer-associated protein Mic1; DE Short=Mic-1; DE AltName: Full=WD repeat-containing protein 98 {ECO:0000305}; GN Name=RMC1 {ECO:0000312|HGNC:HGNC:24326}; GN Synonyms=C18orf8, MIC1, WDR98; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Synovium; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16177791; DOI=10.1038/nature03983; RA Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., RA Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., RA Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J., RA Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., RA Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., RA Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., RA Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., RA Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., RA Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.; RT "DNA sequence and analysis of human chromosome 18."; RL Nature 437:551-555(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA] OF 35-657. RC TISSUE=Colon carcinoma; RA Linnebacher M., Meuer S.C., Rudy W.; RT "Identification of colon-cancer associated sequences by SSH."; RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. RN [5] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=17897319; DOI=10.1111/j.1600-0854.2007.00643.x; RA Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., RA Elsaesser H.-P., Mann M., Hasilik A.; RT "Integral and associated lysosomal membrane proteins."; RL Traffic 8:1676-1686(2007). RN [6] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Erythroleukemia; RX PubMed=23186163; DOI=10.1021/pr300630k; RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., RA Mohammed S.; RT "Toward a comprehensive characterization of a human cancer cell RT phosphoproteome."; RL J. Proteome Res. 12:260-271(2013). RN [7] RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN A COMPLEX WITH RMC1; RP CCZ1; MON1A AND MON1B, SUBCELLULAR LOCATION, AND FUNCTION. RX PubMed=29038162; DOI=10.1128/mcb.00392-17; RA Pontano Vaites L., Paulo J.A., Huttlin E.L., Harper J.W.; RT "Systematic analysis of human cells lacking ATG8 proteins uncovers roles RT for GABARAPs and the CCZ1/MON1 regulator C18orf8/RMC1 in macro and RT selective autophagic flux."; RL Mol. Cell. Biol. 0:0-0(2017). CC -!- FUNCTION: Component of the CCZ1-MON1 RAB7A guanine exchange factor CC (GEF). Acts as a positive regulator of CCZ1-MON1A/B function necessary CC for endosomal/autophagic flux and efficient RAB7A localization CC (PubMed:29038162). {ECO:0000269|PubMed:29038162}. CC -!- SUBUNIT: Found in a complex with RMC1, CCZ1 MON1A and MON1B. CC {ECO:0000269|PubMed:29038162}. CC -!- INTERACTION: CC Q96DM3; Q96A22: C11orf52; NbExp=3; IntAct=EBI-2804848, EBI-6660701; CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:17897319}. CC Late endosome membrane {ECO:0000269|PubMed:29038162}. CC -!- SIMILARITY: Belongs to the RMC1 family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAD33909.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK057192; BAB71377.1; -; mRNA. DR EMBL; BC002950; AAH02950.2; -; mRNA. DR EMBL; BC008305; AAH08305.2; -; mRNA. DR EMBL; AF143536; AAD33909.1; ALT_INIT; mRNA. DR EMBL; AC010853; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC026634; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR CCDS; CCDS32803.1; -. DR RefSeq; NP_001305636.1; NM_001318707.1. DR RefSeq; NP_001305637.1; NM_001318708.1. DR RefSeq; NP_001305638.1; NM_001318709.1. DR RefSeq; NP_037458.3; NM_013326.5. DR PDB; 9L0D; EM; 3.41 A; C=1-657. DR AlphaFoldDB; Q96DM3; -. DR EMDB; EMD-62697; -. DR SMR; Q96DM3; -. DR BioGRID; 118961; 147. DR ComplexPortal; CPX-8152; MON1-CCZ1 guanyl-nucleotide exchange factor complex, MON1B variant. DR ComplexPortal; CPX-8153; MON1-CCZ1 guanyl-nucleotide exchange factor complex, MON1A variant. DR ComplexPortal; CPX-8165; MON1-CCZ1B guanyl-nucleotide exchange factor complex, MON1A variant. DR ComplexPortal; CPX-8166; MON1-CCZ1B guanyl-nucleotide exchange factor complex, MON1B variant. DR CORUM; Q96DM3; -. DR FunCoup; Q96DM3; 2018. DR IntAct; Q96DM3; 131. DR STRING; 9606.ENSP00000269221; -. DR iPTMnet; Q96DM3; -. DR PhosphoSitePlus; Q96DM3; -. DR BioMuta; C18orf8; -. DR DMDM; 47605918; -. DR jPOST; Q96DM3; -. DR MassIVE; Q96DM3; -. DR PaxDb; 9606-ENSP00000269221; -. DR PeptideAtlas; Q96DM3; -. DR ProteomicsDB; 76295; -. DR Pumba; Q96DM3; -. DR Antibodypedia; 22041; 237 antibodies from 33 providers. DR DNASU; 29919; -. DR Ensembl; ENST00000269221.8; ENSP00000269221.2; ENSG00000141452.12. DR GeneID; 29919; -. DR KEGG; hsa:29919; -. DR MANE-Select; ENST00000269221.8; ENSP00000269221.2; NM_013326.5; NP_037458.3. DR UCSC; uc021uie.3; human. DR AGR; HGNC:24326; -. DR ClinPGx; PA134862561; -. DR CTD; 29919; -. DR DisGeNET; 29919; -. DR GeneCards; RMC1; -. DR HGNC; HGNC:24326; RMC1. DR HPA; ENSG00000141452; Low tissue specificity. DR MIM; 620267; gene. DR OpenTargets; ENSG00000141452; -. DR VEuPathDB; HostDB:ENSG00000141452; -. DR eggNOG; KOG2377; Eukaryota. DR GeneTree; ENSGT00390000009127; -. DR InParanoid; Q96DM3; -. DR OMA; AANHHCS; -. DR OrthoDB; 26384at2759; -. DR PAN-GO; Q96DM3; 4 GO annotations based on evolutionary models. DR PhylomeDB; Q96DM3; -. DR PathwayCommons; Q96DM3; -. DR SignaLink; Q96DM3; -. DR SIGNOR; Q96DM3; -. DR Agora; ENSG00000141452; -. DR BioGRID-ORCS; 29919; 37 hits in 1144 CRISPR screens. DR ChiTaRS; C18orf8; human. DR GenomeRNAi; 29919; -. DR Pharos; Q96DM3; Tbio. DR PRO; PR:Q96DM3; -. DR Proteomes; UP000005640; Chromosome 18. DR RNAct; Q96DM3; protein. DR Bgee; ENSG00000141452; Expressed in secondary oocyte and 183 other cell types or tissues. DR ExpressionAtlas; Q96DM3; baseline and differential. DR GO; GO:0005770; C:late endosome; IDA:ComplexPortal. DR GO; GO:0031902; C:late endosome membrane; IDA:UniProtKB. DR GO; GO:0005765; C:lysosomal membrane; HDA:UniProtKB. DR GO; GO:0035658; C:Mon1-Ccz1 complex; IDA:UniProtKB. DR GO; GO:0010506; P:regulation of autophagy; IMP:UniProtKB. DR FunFam; 2.130.10.10:FF:000436; Regulator of MON1-CCZ1 complex; 1. DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1. DR InterPro; IPR040371; RMC1. DR InterPro; IPR009755; RMC1_C. DR InterPro; IPR049040; RMC1_N. DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf. DR InterPro; IPR036322; WD40_repeat_dom_sf. DR PANTHER; PTHR12897; COLON CANCER-ASSOCIATED PROTEIN MIC1; 1. DR PANTHER; PTHR12897:SF4; REGULATOR OF MON1-CCZ1 COMPLEX; 1. DR Pfam; PF07035; RMC1_C; 1. DR Pfam; PF21029; RMC1_N; 1. DR SUPFAM; SSF50978; WD40 repeat-like; 1. PE 1: Evidence at protein level; KW 3D-structure; Autophagy; Endosome; Lysosome; Membrane; KW Proteomics identification; Reference proteome. FT CHAIN 1..657 FT /note="Regulator of MON1-CCZ1 complex" FT /id="PRO_0000096481" FT DOMAIN 471..637 FT /note="Mic1" FT CONFLICT 163 FT /note="S -> R (in Ref. 1; BAB71377)" FT /evidence="ECO:0000305" FT CONFLICT 178 FT /note="Q -> L (in Ref. 4; AAD33909)" FT /evidence="ECO:0000305" FT CONFLICT 470 FT /note="E -> DFFLYGVFKHYRQ (in Ref. 4; AAD33909)" FT /evidence="ECO:0000305" FT HELIX 3..5 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 24..29 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 30..33 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 34..39 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 41..49 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 57..62 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 69..72 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 74..76 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 78..82 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 84..92 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 102..105 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 113..117 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 120..128 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 131..138 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 139..142 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 143..150 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 155..160 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 161..164 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 165..169 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 176..183 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 186..189 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 196..199 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 210..212 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 213..218 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 221..228 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 240..245 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 248..250 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 253..259 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 266..271 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 274..279 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 280..283 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 284..288 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 290..292 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 298..303 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 311..315 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 322..325 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 340..343 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 344..346 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 347..350 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 351..354 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 355..358 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 366..369 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 373..380 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 383..385 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 388..396 FT /evidence="ECO:0007829|PDB:9L0D" FT STRAND 398..402 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 406..435 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 460..465 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 466..470 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 476..492 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 499..511 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 516..523 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 531..539 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 540..542 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 547..558 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 562..571 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 575..585 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 587..589 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 593..598 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 601..603 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 607..610 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 613..624 FT /evidence="ECO:0007829|PDB:9L0D" FT HELIX 631..633 FT /evidence="ECO:0007829|PDB:9L0D" FT TURN 635..637 FT /evidence="ECO:0007829|PDB:9L0D" SQ SEQUENCE 657 AA; 74975 MW; DFDC8B304ADCA88C CRC64; MGEEDYYLEL CERPVQFEKA NPVNCVFFDE ANKQVFAVRS GGATGVVVKG PDDRNPISFR MDDKGEVKCI KFSLENKILA VQRTSKTVDF CNFIPDNSQL EYTQECKTKN ANILGFCWTS STEIVFITDQ GIEFYQVLPE KRSLKLLKSH NLNVNWYMYC PESAVILLST TVLENVLQPF HFRAGTMSKL PKFEIELPAA PKSTKPSLSE RDIAMATIYG QLYVLFLRHH SRTSNSTGAE VVLYHLPREG ACKKMHILKL NRTGKFALNV VDNLVVVHHQ DTETSVIFDI KLRGEFDGSV TFHHPVLPAR SIQPYQIPIT GPAAVTSQSP VPCKLYSSSW IVFQPDIIIS ASQGYLWNLQ VKLEPIVNLL PDKGRLMDFL LQRKECKMVI LSVCSQMLSE SDRASLPVIA TVFDKLNHEY KKYLDAEQSY AMAVEAGQSR SSPLLKRPVR TQAVLDQSDV YTHVLSAFVE KKEMPHKFVI AVLMEYIRSL NQFQIAVQHY LHELVIKTLV QHNLFYMLHQ FLQYHVLSDS KPLACLLLSL ESFYPPAHQL SLDMLKRLST ANDEIVEVLL SKHQVLAALR FIRGIGGHDN ISARKFLDAA KQTEDNMLFY TIFRFFEQRN QRLRGSPNFT PGEHCEEHVA FFKQIFGDQA LMRPTTF //