ID   TF3A_MOUSE              Reviewed;         364 AA.
AC   Q8VHT7; Q8BJ79; Q8K270; Q9CSH8;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   04-NOV-2026, entry version 145.
DE   RecName: Full=Transcription factor IIIA;
DE            Short=TFIIIA;
GN   Name=Gtf3a;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11814676; DOI=10.1016/s0378-1119(01)00796-x;
RA   Hanas J.S., Hocker J.R., Cheng Y.-G., Lerner M.R., Brackett D.J.,
RA   Lightfoot S.A., Hanas R.J., Madhusudhan K.T., Moreland R.J.;
RT   "cDNA cloning, DNA binding, and evolution of mammalian transcription factor
RT   IIIA.";
RL   Gene 282:43-52(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Small intestine;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-364.
RC   STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Involved in ribosomal large subunit biogenesis. Binds the
CC       approximately 50 base pairs internal control region (ICR) of 5S
CC       ribosomal RNA genes. It is required for their RNA polymerase III-
CC       dependent transcription and may also maintain the transcription of
CC       other genes (By similarity). Also binds the transcribed 5S RNA's (By
CC       similarity). {ECO:0000250|UniProtKB:P17842,
CC       ECO:0000250|UniProtKB:Q92664}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- CAUTION: It is uncertain whether Met-1 is the initiator. Based on the
CC       lack of an in-frame AUG codon, mammalian TFIIIA may be translated from
CC       this non-AUG initiation site, which has a good Kozak context and which
CC       is well conserved among mammals. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL69686.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB28476.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAC25209.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF391799; AAL69686.1; ALT_INIT; mRNA.
DR   EMBL; AK008247; BAC25209.1; ALT_INIT; mRNA.
DR   EMBL; AK012797; BAB28476.2; ALT_INIT; mRNA.
DR   EMBL; BC032292; AAH32292.1; -; mRNA.
DR   CCDS; CCDS19873.2; -.
DR   RefSeq; NP_079928.2; NM_025652.3.
DR   AlphaFoldDB; Q8VHT7; -.
DR   SMR; Q8VHT7; -.
DR   FunCoup; Q8VHT7; 2370.
DR   STRING; 10090.ENSMUSP00000119607; -.
DR   GlyCosmos; Q8VHT7; 1 site, 1 glycan.
DR   GlyGen; Q8VHT7; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q8VHT7; -.
DR   PhosphoSitePlus; Q8VHT7; -.
DR   PaxDb; 10090-ENSMUSP00000119607; -.
DR   ProteomicsDB; 259014; -.
DR   Pumba; Q8VHT7; -.
DR   Antibodypedia; 5062; 255 antibodies from 27 providers.
DR   DNASU; 66596; -.
DR   Ensembl; ENSMUST00000146511.8; ENSMUSP00000119607.2; ENSMUSG00000016503.19.
DR   GeneID; 66596; -.
DR   KEGG; mmu:66596; -.
DR   UCSC; uc009ano.3; mouse.
DR   AGR; MGI:1913846; -.
DR   CTD; 2971; -.
DR   MGI; MGI:1913846; Gtf3a.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000155647; -.
DR   InParanoid; Q8VHT7; -.
DR   OMA; AAHIYMH; -.
DR   OrthoDB; 2687452at2759; -.
DR   PhylomeDB; Q8VHT7; -.
DR   Reactome; R-MMU-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR   BioGRID-ORCS; 66596; 21 hits in 81 CRISPR screens.
DR   PRO; PR:Q8VHT7; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; Q8VHT7; protein.
DR   Bgee; ENSMUSG00000016503; Expressed in otic placode and 271 other cell types or tissues.
DR   ExpressionAtlas; Q8VHT7; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; ISO:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0008097; F:5S rRNA binding; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IDA:MGI.
DR   GO; GO:0000995; F:RNA polymerase III general transcription initiation factor activity; ISO:GO_Central.
DR   GO; GO:0042273; P:ribosomal large subunit biogenesis; ISS:UniProtKB.
DR   FunFam; 3.30.160.60:FF:001572; General transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:001810; General transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:000125; Putative zinc finger protein 143; 1.
DR   FunFam; 3.30.160.60:FF:001102; Transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:001347; Transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:001998; Transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:001610; transcription factor IIIA; 1.
DR   FunFam; 3.30.160.60:FF:000446; Zinc finger protein; 1.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 9.
DR   InterPro; IPR054599; TFIIIA_Zfn-C2H2.
DR   InterPro; IPR013087; Zf-C2H2_type.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   PANTHER; PTHR46179:SF1; TRANSCRIPTION FACTOR IIIA; 1.
DR   PANTHER; PTHR46179; ZINC FINGER PROTEIN; 1.
DR   Pfam; PF22110; TFIIIA_zf-C2H2; 1.
DR   Pfam; PF00096; zf-C2H2; 5.
DR   SMART; SM00355; ZnF_C2H2; 9.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 6.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 8.
PE   2: Evidence at transcript level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Ribosome biogenesis; RNA-binding; Transcription; Transcription regulation;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..364
FT                   /note="Transcription factor IIIA"
FT                   /id="PRO_0000319866"
FT   ZN_FING         38..62
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         68..92
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         98..123
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         130..154
FT                   /note="C2H2-type 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         160..184
FT                   /note="C2H2-type 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         187..211
FT                   /note="C2H2-type 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         215..237
FT                   /note="C2H2-type 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         244..269
FT                   /note="C2H2-type 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         275..299
FT                   /note="C2H2-type 9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          299..364
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        338..352
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        75..77
FT                   /note="CGK -> HAS (in Ref. 3; AAH32292)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131
FT                   /note="V -> L (in Ref. 1; AAL69686)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149..173
FT                   /note="THQCQHTSEPLFRCTHEGCGKHFAS -> PISASTPASRSSGVPTRDAGSTL
FT                   PR (in Ref. 2; BAC25209)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194
FT                   /note="S -> P (in Ref. 2; BAC25209)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        198
FT                   /note="K -> I (in Ref. 2; BAC25209)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        359
FT                   /note="A -> T (in Ref. 3; AAH32292)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   364 AA;  41571 MW;  FEC01712C5C8D4DA CRC64;
     MEPRVSVAEA VSSLTIADAF VGACEGPAPP RPALPSRFIC SFPDCSASYN KAWKLDAHLC
     KHTGERPFVC DYEGCGKAFI RDYHLSRHVL IHTGEKPFVC ADDGCNQKFN TKSNLKKHIE
     RKHGNPQKQY VCSYEGCKKA FKKHQQLRTH QCQHTSEPLF RCTHEGCGKH FASPSRLKRH
     GKVHEGYLCQ KGCSFMGKTW TELLKHMREA HKEDITCNVC QRMFKRRDYL KQHMKTHAPE
     RDVYRCPRQG CGRTYTTVFN LQSHILSFHE EKRPFVCEHA GCGKTFAMKQ SLMRHSVVHD
     PDKKRMKLKV RAPRERRSLA SRLSGYFPPK RKQEPDYSLP NASAESSSSP EAQLPPPAAL
     LTVC
//