Q8U8T9 BIOD_AGRFC ATP-dependent dethiobiotin synthetase BioD 6.3.3.3 DTB synthetase DTBS Dethiobiotin synthase bioD Atu3999 AGR_L_1705 Agrobacterium fabrum (strain C58 / ATCC 33970) Agrobacterium tumefaciens (strain C58) Bacteria Pseudomonadati Pseudomonadota Alphaproteobacteria Hyphomicrobiales Rhizobiaceae Rhizobium/Agrobacterium group Agrobacterium Agrobacterium tumefaciens complex NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] C58 / ATCC 33970 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] C58 / ATCC 33970 Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring. (7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H(+) Mg(2+) Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. Homodimer. Cytoplasm Belongs to the dethiobiotin synthetase family. ATP-binding Biotin biosynthesis Cytoplasm Ligase Magnesium Metal-binding Nucleotide-binding Reference proteome ATP Mg(2+) substrate ATP Mg(2+) ATP MSLRFVISGTDTGIGKTVFAAALTHALEAHYWKPVQSGLEEETDSQTVARLAGASHARILPEAYRLKTPASPHLSARLDNVSIDPARLLPPRPDGPLVIEGAGGLLVPLTDRLLFADIFALWQIPLILCARTALGTINHTLLSLEALRHRAIPVQGVVFIGDEDRENERVITDIGAVRRLGRLPRLPELTPEALHQAFAQHFNLADFLEVPA Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License