ID   BYST_ARATH              Reviewed;         444 AA.
AC   Q8RWS4; Q9C6V6;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   02-SEP-2026, entry version 135.
DE   RecName: Full=Bystin {ECO:0000305};
DE   AltName: Full=Essential nuclear protein 1 {ECO:0000303|PubMed:23382868};
DE            Short=AtEnp1 {ECO:0000303|PubMed:23382868};
GN   Name=ENP1 {ECO:0000303|PubMed:23382868};
GN   OrderedLocusNames=At1g31660 {ECO:0000312|Araport:AT1G31660};
GN   ORFNames=F27M3.14 {ECO:0000312|EMBL:AAG60158.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP   AND SUBUNIT.
RC   STRAIN=cv. Columbia;
RX   PubMed=23382868; DOI=10.1371/journal.pone.0054084;
RA   Missbach S., Weis B.L., Martin R., Simm S., Bohnsack M.T., Schleiff E.;
RT   "40S ribosome biogenesis co-factors are essential for gametophyte and
RT   embryo development.";
RL   PLoS ONE 8:E54084-E54084(2013).
CC   -!- FUNCTION: Essential protein required during embryogenesis and pollen
CC       development (PubMed:23382868). Required for processing of 20S pre-rRNA
CC       precursor and biogenesis of 40S ribosomal subunits (PubMed:23382868).
CC       {ECO:0000269|PubMed:23382868}.
CC   -!- SUBUNIT: Component of the 40S pre-ribosome.
CC       {ECO:0000269|PubMed:23382868}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:23382868}.
CC       Nucleus, nucleoplasm {ECO:0000269|PubMed:23382868}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in flowers and at lower levels in
CC       roots, hypocotyls, stems, leaves, siliques and seeds.
CC       {ECO:0000269|PubMed:23382868}.
CC   -!- DISRUPTION PHENOTYPE: Delayed pollen development with reduced nuclei
CC       content leading to reduced pollen germination capacity, and pale seeds
CC       with arrested embryo development at the globular stage in homozygous
CC       plants (PubMed:23382868). In heterozygous plants, slight accumulation
CC       of the 23S-like precursor P-A3 (PubMed:23382868). Reduced siliques size
CC       with small non-developed and early aborted seeds (PubMed:23382868).
CC       {ECO:0000269|PubMed:23382868}.
CC   -!- SIMILARITY: Belongs to the bystin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG60158.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC074360; AAG60158.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE31381.1; -; Genomic_DNA.
DR   EMBL; OZ408683; CAO7029344.1; -; Genomic_DNA.
DR   EMBL; AY091144; AAM14093.1; -; mRNA.
DR   EMBL; BT003812; AAO41865.1; -; mRNA.
DR   RefSeq; NP_174447.2; NM_102901.4.
DR   AlphaFoldDB; Q8RWS4; -.
DR   SMR; Q8RWS4; -.
DR   FunCoup; Q8RWS4; 2954.
DR   STRING; 3702.Q8RWS4; -.
DR   PaxDb; 3702-Q8RWS4; -.
DR   ProteomicsDB; 179361; -.
DR   GeneID; 840053; -.
DR   KEGG; ath:AT1G31660; -.
DR   Araport; AT1G31660; -.
DR   TAIR; AT1G31660; ENP1.
DR   eggNOG; KOG3871; Eukaryota.
DR   HOGENOM; CLU_029727_0_1_1; -.
DR   InParanoid; Q8RWS4; -.
DR   OMA; TKLPVIW; -.
DR   PhylomeDB; Q8RWS4; -.
DR   CD-CODE; 4299E36E; Nucleolus.
DR   PRO; PR:Q8RWS4; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISM:TAIR.
DR   GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR   GO; GO:0030515; F:snoRNA binding; IBA:GO_Central.
DR   GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR   GO; GO:0009555; P:pollen development; IMP:TAIR.
DR   GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR   FunFam; 1.25.40.480:FF:000001; Bystin (51.6 kD)-like; 1.
DR   Gene3D; 1.25.40.480; -; 1.
DR   InterPro; IPR007955; Bystin.
DR   PANTHER; PTHR12821; BYSTIN; 1.
DR   PANTHER; PTHR12821:SF0; BYSTIN; 1.
DR   Pfam; PF05291; Bystin; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing.
FT   CHAIN           1..444
FT                   /note="Bystin"
FT                   /id="PRO_0000448722"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          53..85
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..34
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        73..84
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   444 AA;  51473 MW;  18646DF14AF97F57 CRC64;
     MAKKRDRIVN TQPFISDDAS VASSRKRSKV PKTHQKQEKL IEAGMSEKIM KQALAQQKEV
     ADEENAERNP SSAAFAVAGA ATAGEEQKIL EEEEDDIDDF DGTFENQSQF DKQEEINEDD
     EKLFESFLNK NAPPQRTLTD IIIKKLKDKD ADLAEEERPD PKMDPAITKL YKGVGKFMSE
     YTVGKLPKAF KLVTSMEHWE DVLYLTEPEK WSPNALYQAT RIFASNLKDR QVQRFYNYVL
     LPRVREDIRK HKKLHFALYQ ALKKSLYKPS AFNQGILFPL CKSGTCNLRE AVIIGSILEK
     CSIPMLHSCV ALNRLAEMDY CGTTSYFIKV LLEKKYCMPY RVLDALVAHF MRFVDDIRVM
     PVIWHQSLLT FVQRYKYEIL KEDKEHLQTL LQRQKHHLVT PEILRELKDS RNRGEKEDPM
     VDNFAPVPAK EDRFDIPEVP MEED
//