ID   HYCC2_HUMAN             Reviewed;         530 AA.
AC   Q8IXS8; B2RCG7; Q4ZG87; Q53TX6;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   04-NOV-2026, entry version 152.
DE   RecName: Full=Hyccin 2 {ECO:0000305};
GN   Name=HYCC2 {ECO:0000312|HGNC:HGNC:28593}; Synonyms=FAM126B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Fetal brain;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Adipose tissue;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-30; THR-306; SER-321;
RP   SER-430; SER-442; SER-444 AND SER-491, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [8]
RP   IDENTIFICATION IN THE PI4K COMPLEX.
RX   PubMed=26571211; DOI=10.1038/ncb3271;
RA   Baskin J.M., Wu X., Christiano R., Oh M.S., Schauder C.M., Gazzerro E.,
RA   Messa M., Baldassari S., Assereto S., Biancheri R., Zara F., Minetti C.,
RA   Raimondi A., Simons M., Walther T.C., Reinisch K.M., De Camilli P.;
RT   "The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis
RT   at the plasma membrane.";
RL   Nat. Cell Biol. 18:132-138(2016).
CC   -!- FUNCTION: Component of a complex required to localize
CC       phosphatidylinositol 4-kinase (PI4K) to the plasma membrane.
CC       {ECO:0000305|PubMed:26571211}.
CC   -!- SUBUNIT: Component of a phosphatidylinositol 4-kinase (PI4K) complex,
CC       composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and
CC       HYCC (HYCC1 or HYCC2). {ECO:0000269|PubMed:26571211}.
CC   -!- INTERACTION:
CC       Q8IXS8; Q08379: GOLGA2; NbExp=3; IntAct=EBI-8787606, EBI-618309;
CC       Q8IXS8; P50221: MEOX1; NbExp=3; IntAct=EBI-8787606, EBI-2864512;
CC       Q8IXS8; P14373: TRIM27; NbExp=3; IntAct=EBI-8787606, EBI-719493;
CC       Q8IXS8; Q86TV6: TTC7B; NbExp=3; IntAct=EBI-8787606, EBI-12006098;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9BYI3}. Cell membrane
CC       {ECO:0000250|UniProtKB:Q9BYI3}.
CC   -!- SIMILARITY: Belongs to the Hyccin family. {ECO:0000305}.
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DR   EMBL; AK315104; BAG37564.1; -; mRNA.
DR   EMBL; AL833403; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AC005037; AAY14726.1; -; Genomic_DNA.
DR   EMBL; AC007272; AAX88971.1; -; Genomic_DNA.
DR   EMBL; CH471063; EAW70232.1; -; Genomic_DNA.
DR   EMBL; BC039295; AAH39295.1; -; mRNA.
DR   CCDS; CCDS2335.1; -.
DR   RefSeq; NP_001308547.1; NM_001321618.1.
DR   RefSeq; NP_001308548.1; NM_001321619.2.
DR   RefSeq; NP_001308550.1; NM_001321621.2.
DR   RefSeq; NP_001308551.1; NM_001321622.2.
DR   RefSeq; NP_001308556.1; NM_001321627.1.
DR   RefSeq; NP_776183.1; NM_173822.5.
DR   RefSeq; XP_016859370.1; XM_017003881.2.
DR   RefSeq; XP_047299959.1; XM_047444003.1.
DR   RefSeq; XP_047299960.1; XM_047444004.1.
DR   RefSeq; XP_054197490.1; XM_054341515.1.
DR   RefSeq; XP_054197491.1; XM_054341516.1.
DR   RefSeq; XP_054197492.1; XM_054341517.1.
DR   AlphaFoldDB; Q8IXS8; -.
DR   SMR; Q8IXS8; -.
DR   BioGRID; 130030; 21.
DR   FunCoup; Q8IXS8; 1182.
DR   IntAct; Q8IXS8; 14.
DR   STRING; 9606.ENSP00000393667; -.
DR   GlyCosmos; Q8IXS8; 2 sites, 1 glycan.
DR   GlyGen; Q8IXS8; 2 sites, 1 O-linked glycan (2 sites).
DR   iPTMnet; Q8IXS8; -.
DR   PhosphoSitePlus; Q8IXS8; -.
DR   BioMuta; FAM126B; -.
DR   DMDM; 74728235; -.
DR   jPOST; Q8IXS8; -.
DR   MassIVE; Q8IXS8; -.
DR   PaxDb; 9606-ENSP00000393667; -.
DR   PeptideAtlas; Q8IXS8; -.
DR   ProteomicsDB; 71059; -.
DR   Pumba; Q8IXS8; -.
DR   Antibodypedia; 50921; 46 antibodies from 10 providers.
DR   DNASU; 285172; -.
DR   Ensembl; ENST00000418596.7; ENSP00000393667.2; ENSG00000155744.11.
DR   Ensembl; ENST00000861781.1; ENSP00000531840.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861782.1; ENSP00000531841.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861783.1; ENSP00000531842.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861788.1; ENSP00000531847.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861789.1; ENSP00000531848.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861790.1; ENSP00000531849.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861794.1; ENSP00000531853.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861795.1; ENSP00000531854.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861797.1; ENSP00000531856.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861798.1; ENSP00000531857.1; ENSG00000155744.11.
DR   Ensembl; ENST00000861800.1; ENSP00000531859.1; ENSG00000155744.11.
DR   Ensembl; ENST00000944415.1; ENSP00000614474.1; ENSG00000155744.11.
DR   Ensembl; ENST00000944416.1; ENSP00000614475.1; ENSG00000155744.11.
DR   Ensembl; ENST00000944417.1; ENSP00000614476.1; ENSG00000155744.11.
DR   Ensembl; ENST00000944418.1; ENSP00000614477.1; ENSG00000155744.11.
DR   GeneID; 285172; -.
DR   KEGG; hsa:285172; -.
DR   UCSC; uc002uws.4; human.
DR   AGR; HGNC:28593; -.
DR   ClinPGx; PA162385879; -.
DR   CTD; 285172; -.
DR   DisGeNET; 285172; -.
DR   GeneCards; HYCC2; -.
DR   HGNC; HGNC:28593; HYCC2.
DR   HPA; ENSG00000155744; Low tissue specificity.
DR   OpenTargets; ENSG00000155744; -.
DR   VEuPathDB; HostDB:ENSG00000155744; -.
DR   eggNOG; KOG4688; Eukaryota.
DR   GeneTree; ENSGT00390000011295; -.
DR   HOGENOM; CLU_027457_3_0_1; -.
DR   InParanoid; Q8IXS8; -.
DR   OMA; AAVYMPC; -.
DR   OrthoDB; 18937at2759; -.
DR   PAN-GO; Q8IXS8; 3 GO annotations based on evolutionary models.
DR   PhylomeDB; Q8IXS8; -.
DR   PathwayCommons; Q8IXS8; -.
DR   SignaLink; Q8IXS8; -.
DR   Agora; ENSG00000155744; -.
DR   BioGRID-ORCS; 285172; 21 hits in 1163 CRISPR screens.
DR   ChiTaRS; FAM126B; human.
DR   GenomeRNAi; 285172; -.
DR   Pharos; Q8IXS8; Tdark.
DR   PRO; PR:Q8IXS8; -.
DR   Proteomes; UP000005640; Chromosome 2.
DR   RNAct; Q8IXS8; protein.
DR   Bgee; ENSG00000155744; Expressed in buccal mucosa cell and 192 other cell types or tissues.
DR   ExpressionAtlas; Q8IXS8; baseline and differential.
DR   GO; GO:0030054; C:cell junction; IDA:HPA.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR   InterPro; IPR018619; Hyccin.
DR   PANTHER; PTHR31220:SF3; HYCCIN 2; 1.
DR   PANTHER; PTHR31220; HYCCIN RELATED; 1.
DR   Pfam; PF09790; Hyccin; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cytoplasm; Membrane; Phosphoprotein;
KW   Proteomics identification; Reference proteome.
FT   CHAIN           1..530
FT                   /note="Hyccin 2"
FT                   /id="PRO_0000278094"
FT   REGION          328..410
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          502..530
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        353..373
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        514..530
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         30
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         306
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         321
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163,
FT                   ECO:0007744|PubMed:24275569"
FT   MOD_RES         341
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8C729"
FT   MOD_RES         430
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         442
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         444
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         491
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
SQ   SEQUENCE   530 AA;  58647 MW;  FD448E6AAB39D5AC CRC64;
     MLGTDRCVVE EWLSEFKALP DTQITSYAAT LHRKKTLVPA LYKVIQDSNN ELLEPVCHQL
     FELYRSSEVR LKRFTLQFLP ELMWVYLRLT VSRDRQSNGC IEALLLGIYN LEIADKDGNN
     KVLSFTIPSL SKPSIYHEPS TIGSMALTEG ALCQHDLIRV VYSDLHPQRE TFTAQNRFEV
     LSFLMLCYNS AIVYMPASSY QSLCRMGSRV CVSGFPRQHE KHWKELCGRI VLDPEFMVQL
     LTGVYYAMYN GQWDLGQEVL DDIIYRAQLE LFSQPLLVAN AMKNSLPFDA PDSTQEGQKV
     LKVEVTPTVP RISRTAITTA SIRRHRWRRE GAEGVNGGEE SVNLNDADEG FSSGASLSSQ
     PIGTKPSSSS QRGSLRKVAT GRSAKDKETA SAIKSSESPR DSVVRKQYVQ QPTDLSVDSV
     ELTPMKKHLS LPAGQVVPKI NSLSLIRTAS ASSSKSFDYV NGSQASTSIG VGTEGGTNLA
     ANNANRYSTV SLQEDRLGQA GEGKELLSPG APLTKQSRSP SFNMQLISQV
//