ID ECHA_MOUSE Reviewed; 763 AA. AC Q8BMS1; Q3TCY3; Q5U5Y5; Q8QZU4; DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2003, sequence version 1. DT 04-NOV-2026, entry version 198. DE RecName: Full=Trifunctional enzyme subunit alpha, mitochondrial; DE AltName: Full=Monolysocardiolipin acyltransferase {ECO:0000250|UniProtKB:P40939}; DE Short=MLCL AT {ECO:0000305}; DE EC=2.3.1.- {ECO:0000250|UniProtKB:P40939}; DE AltName: Full=TP-alpha; DE Includes: DE RecName: Full=Long-chain enoyl-CoA hydratase; DE Short=LCEH; DE EC=4.2.1.17 {ECO:0000250|UniProtKB:P40939}; DE Includes: DE RecName: Full=Long chain 3-hydroxyacyl-CoA dehydrogenase; DE Short=LCHAD; DE EC=1.1.1.211 {ECO:0000250|UniProtKB:P40939}; DE Flags: Precursor; GN Name=Hadha; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J, and NOD; TISSUE=Skin; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=FVB/N; TISSUE=Eye, Liver, and Olfactory epithelium; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-129, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=16916647; DOI=10.1016/j.molcel.2006.06.026; RA Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., RA Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.; RT "Substrate and functional diversity of lysine acetylation revealed by a RT proteomics survey."; RL Mol. Cell 23:607-618(2006). RN [4] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-231; SER-316; THR-395 AND RP SER-647, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, RC Pancreas, Spleen, and Testis; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). RN [5] RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-60 AND LYS-406, SUCCINYLATION RP [LARGE SCALE ANALYSIS] AT LYS-46; LYS-60; LYS-166; LYS-213; LYS-214; RP LYS-230; LYS-249; LYS-303; LYS-326; LYS-334; LYS-350; LYS-406; LYS-411; RP LYS-415; LYS-436; LYS-440; LYS-460; LYS-505; LYS-519; LYS-569; LYS-620; RP LYS-634; LYS-644; LYS-646; LYS-664; LYS-728 AND LYS-759, AND IDENTIFICATION RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Embryonic fibroblast, and Liver; RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001; RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.; RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic RT pathways."; RL Mol. Cell 50:919-930(2013). RN [6] RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-46; LYS-60; LYS-129; LYS-166; RP LYS-214; LYS-249; LYS-289; LYS-303; LYS-326; LYS-334; LYS-350; LYS-353; RP LYS-406; LYS-411; LYS-436; LYS-460; LYS-505; LYS-519; LYS-540; LYS-569; RP LYS-644; LYS-664; LYS-728; LYS-735 AND LYS-759, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Liver; RX PubMed=23576753; DOI=10.1073/pnas.1302961110; RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.; RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria RT identifies substrates of SIRT3 in metabolic pathways."; RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013). RN [7] RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-399, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain; RX PubMed=24129315; DOI=10.1074/mcp.o113.027870; RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M., RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V., RA Bedford M.T., Comb M.J.; RT "Immunoaffinity enrichment and mass spectrometry analysis of protein RT methylation."; RL Mol. Cell. Proteomics 13:372-387(2014). RN [8] RP INTERACTION WITH MTLN, AND IDENTIFICATION BY MASS SPECTROMETRY. RX PubMed=29949755; DOI=10.1016/j.celrep.2018.05.058; RA Makarewich C.A., Baskin K.K., Munir A.Z., Bezprozvannaya S., Sharma G., RA Khemtong C., Shah A.M., McAnally J.R., Malloy C.R., Szweda L.I., RA Bassel-Duby R., Olson E.N.; RT "MOXI Is a Mitochondrial Micropeptide That Enhances Fatty Acid beta- RT Oxidation."; RL Cell Rep. 23:3701-3709(2018). CC -!- FUNCTION: Mitochondrial trifunctional enzyme catalyzes the last three CC of the four reactions of the mitochondrial beta-oxidation pathway. The CC mitochondrial beta-oxidation pathway is the major energy-producing CC process in tissues and is performed through cycles of four consecutive CC reactions. Each beta-oxidation cycle shortens the fatty acyl-CoA by two CC carbons, yielding one acetyl-CoA (for the citric acid cycle), one CC FADH(2), and one NADH (which donate electrons to the respiratory chain CC for ATP production). These cycles repeat until the chain is fully CC degraded to acetyl-CoA units. Among the enzymes involved in this CC pathway, the trifunctional protein--responsible for the hydration, CC dehydrogenation, and thiolysis steps, shows specificity for long-chain CC fatty acids, such as those from dietary and stored fats. Mitochondrial CC trifunctional enzyme is a heterotetrameric complex composed of two CC proteins, the trifunctional enzyme subunit alpha/HADHA described here CC carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA CC dehydrogenase activities while the trifunctional enzyme subunit CC beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. These activities CC have been experimentally confirmed on a few substrates derived from CC beta-oxidation of long-chain saturated fatty acids such as palmitate CC (hexadecanoate) and laurate (dodecanoate). In addition, based on its CC established catalytic mechanism, and combined genetic interaction or CC mutant phenotype evidence, it is predicted to act also on other CC substrates, including long-chain unsaturated fatty acids such as oleate CC (9Z-octadecenoate), linoleate (9Z,12Z-octadecadienoate), linolenate CC (9Z,12Z,15Z-octadecatrienoate), and others. Independently of subunit CC beta, HADHA also exhibits a cardiolipin acyltransferase activity that CC participates in cardiolipin remodeling; cardiolipin is a major CC mitochondrial membrane phospholipid. HADHA may act downstream of CC Tafazzin/TAZ, that remodels monolysocardiolipin (MLCL) to a cardiolipin CC intermediate, and then HADHA may continue to remodel this species into CC mature tetralinoleoyl-cardiolipin. Has also been proposed to act CC directly on MLCL; capable of acylating MLCL using different acyl-CoA CC substrates, with highest activity for oleoyl-CoA. CC {ECO:0000250|UniProtKB:P40939}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; CC Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, CC ChEBI:CHEBI:58856; EC=4.2.1.17; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:16107; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a long-chain (3S)-3-hydroxy fatty acyl-CoA + NAD(+) = a long- CC chain 3-oxo-fatty acyl-CoA + NADH + H(+); Xref=Rhea:RHEA:52656, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:136757, ChEBI:CHEBI:136758; EC=1.1.1.211; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:52657; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3- CC phospho]-glycerol + an acyl-CoA = a cardiolipin + CoA; CC Xref=Rhea:RHEA:35839, ChEBI:CHEBI:57287, ChEBI:CHEBI:58342, CC ChEBI:CHEBI:62237, ChEBI:CHEBI:64743; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35840; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyeicosanoyl-CoA = (2E)-eicosenoyl-CoA + H2O; CC Xref=Rhea:RHEA:78591, ChEBI:CHEBI:15377, ChEBI:CHEBI:74691, CC ChEBI:CHEBI:87562; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78593; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyoctadecanoyl-CoA = (2E)-octadecenoyl-CoA + H2O; CC Xref=Rhea:RHEA:78603, ChEBI:CHEBI:15377, ChEBI:CHEBI:71412, CC ChEBI:CHEBI:87561; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78605; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O; CC Xref=Rhea:RHEA:31163, ChEBI:CHEBI:15377, ChEBI:CHEBI:61526, CC ChEBI:CHEBI:62613; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31165; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxytetradecanoyl-CoA = (2E)-tetradecenoyl-CoA + H2O; CC Xref=Rhea:RHEA:31171, ChEBI:CHEBI:15377, ChEBI:CHEBI:61405, CC ChEBI:CHEBI:62614; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31173; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O; CC Xref=Rhea:RHEA:31075, ChEBI:CHEBI:15377, ChEBI:CHEBI:57330, CC ChEBI:CHEBI:62558; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31077; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O; CC Xref=Rhea:RHEA:31191, ChEBI:CHEBI:15377, ChEBI:CHEBI:61406, CC ChEBI:CHEBI:62616; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31193; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyoctanoyl-CoA = (2E)-octenoyl-CoA + H2O; CC Xref=Rhea:RHEA:31199, ChEBI:CHEBI:15377, ChEBI:CHEBI:62242, CC ChEBI:CHEBI:62617; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:31201; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O; CC Xref=Rhea:RHEA:30547, ChEBI:CHEBI:15377, ChEBI:CHEBI:62075, CC ChEBI:CHEBI:62077; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:30549; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,11Z)-eicosadienoyl-CoA + H2O = (3S)-hydroxy-(11Z)- CC eicosenoyl-CoA; Xref=Rhea:RHEA:83067, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:76388, ChEBI:CHEBI:232717; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83068; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,9Z)-octadecadienoyl-CoA + H2O = (3S)-hydroxy-(9Z- CC octadecenoyl)-CoA; Xref=Rhea:RHEA:47424, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:77553, ChEBI:CHEBI:87687; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47425; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,7Z)-hexadecadienoyl-CoA + H2O = (3S)-hydroxy-(7Z- CC hexadecenoyl)-CoA; Xref=Rhea:RHEA:47436, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:87717, ChEBI:CHEBI:87718; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47437; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,5Z)-tetradecadienoyl-CoA + H2O = (3S)-hydroxy-(5Z- CC tetradecenoyl)-CoA; Xref=Rhea:RHEA:47452, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:87701, ChEBI:CHEBI:87705; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47453; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,11Z)-octadecadienoyl-CoA + H2O = (3S)-hydroxy-(11Z)- CC octadecenoyl-CoA; Xref=Rhea:RHEA:83027, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:76558, ChEBI:CHEBI:232532; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83028; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,9Z)-hexadecadienoyl-CoA + H2O = (3S)-hydroxy-(9Z- CC hexadecenoyl)-CoA; Xref=Rhea:RHEA:82923, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:77549, ChEBI:CHEBI:232641; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:82924; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,7Z)-tetradecadienoyl-CoA + H2O = (3S)-hydroxy-(7Z)- CC tetradecenoyl-CoA; Xref=Rhea:RHEA:82995, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:231719, ChEBI:CHEBI:232680; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:82996; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,5Z)-dodecadienoyl-CoA + H2O = (3S)-hydroxy-(5Z)- CC dodecenoyl-CoA; Xref=Rhea:RHEA:82959, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232684, ChEBI:CHEBI:232686; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:82960; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z)-hexadecadienoyl-CoA + H2O = (3S)-hydroxy-(6Z)- CC hexadecenoyl-CoA; Xref=Rhea:RHEA:83147, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232761, ChEBI:CHEBI:232762; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83148; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z)-octadecadienoyl-CoA + H2O = (3S)-hydroxy-(6Z)- CC octadecenoyl-CoA; Xref=Rhea:RHEA:83211, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232772, ChEBI:CHEBI:232773; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83212; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,9Z)-eicosadienoyl-CoA + H2O = (3S)-hydroxy-(9Z)- CC eicosenoyl-CoA; Xref=Rhea:RHEA:83359, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232892, ChEBI:CHEBI:232893; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83360; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,7Z)-octadecadienoyl-CoA + H2O = (3S)-hydroxy-(7Z)- CC octadecenoyl-CoA; Xref=Rhea:RHEA:83375, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232899, ChEBI:CHEBI:232900; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83376; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,5Z)-hexadecadienoyl-CoA + H2O = (3S)-hydroxy-(5Z)- CC hexadecenoyl-CoA; Xref=Rhea:RHEA:83391, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:232910, ChEBI:CHEBI:232911; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83392; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z,12Z)-octadecadienoyl-CoA = (2E,9Z,12Z)- CC octadecatrienoyl-CoA + H2O; Xref=Rhea:RHEA:84395, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:77558, ChEBI:CHEBI:233824; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:84397; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z,10Z)-hexadecadienoyl-CoA = (2E,7Z,10Z)- CC hexadecatrienoyl-CoA + H2O; Xref=Rhea:RHEA:84399, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:233785, ChEBI:CHEBI:233826; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:84401; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,8Z)-tetradecadienoyl-CoA + H2O = (3S)-hydroxy-(8Z)- CC tetradecenoyl-CoA; Xref=Rhea:RHEA:84403, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:233831, ChEBI:CHEBI:233832; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84404; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z)-dodecadienoyl-CoA + H2O = (3S)-hydroxy-(6Z)- CC dodecenoyl-CoA; Xref=Rhea:RHEA:84407, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:233835, ChEBI:CHEBI:233836; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84408; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,9Z,12Z,15Z)-octadecatetraenoyl-CoA + H2O = (3S)-hydroxy- CC (9Z,12Z,15Z)-octadecatrienoyl-CoA; Xref=Rhea:RHEA:84627, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:233840, ChEBI:CHEBI:233841; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84628; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,7Z,10Z,13Z)-hexadecatetraenoyl-CoA + H2O = (3S)-hydroxy- CC (7Z,10Z,13Z)-hexadecatrienoyl-CoA; Xref=Rhea:RHEA:84631, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:233792, ChEBI:CHEBI:233843; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84632; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,8Z,11Z)-tetradecatrienoyl-CoA + H2O = (3S)-hydroxy- CC (8Z,11Z)-tetradecadienoyl-CoA; Xref=Rhea:RHEA:84655, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:233801, ChEBI:CHEBI:233844; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84656; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z,9Z)-dodecatrienoyl-CoA + H2O = (3S)-hydroxy-(6Z,9Z)- CC dodecadienoyl-CoA; Xref=Rhea:RHEA:84659, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:233847, ChEBI:CHEBI:233848; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84660; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,7Z)-decadienoyl-CoA + H2O = (3S)-hydroxy-(7Z)-decenoyl- CC CoA; Xref=Rhea:RHEA:84663, ChEBI:CHEBI:15377, ChEBI:CHEBI:233853, CC ChEBI:CHEBI:233854; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84664; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z,9Z,12Z)-octadecatetraenoyl-CoA + H2O = (3S)-hydroxy- CC (6Z,9Z,12Z)-octadecatrienoyl-CoA; Xref=Rhea:RHEA:84667, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:233778, ChEBI:CHEBI:233839; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84668; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E,6Z,9Z,12Z,15Z)-octadecapentaenoyl-CoA + H2O = (3S)- CC hydroxy-(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA; Xref=Rhea:RHEA:85127, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:233740, ChEBI:CHEBI:234230; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:85128; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyeicosanoyl-CoA + NAD(+) = 3-oxoeicosanoyl-CoA + CC NADH + H(+); Xref=Rhea:RHEA:78731, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65115, CC ChEBI:CHEBI:87562; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78732; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyoctadecanoyl-CoA + NAD(+) = 3-oxooctadecanoyl-CoA CC + NADH + H(+); Xref=Rhea:RHEA:78739, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71407, CC ChEBI:CHEBI:87561; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78740; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA CC + NADH + H(+); Xref=Rhea:RHEA:31159, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57349, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:62613; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31160; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl- CC CoA + NADH + H(+); Xref=Rhea:RHEA:31167, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62543, CC ChEBI:CHEBI:62614; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31168; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA + CC NADH + H(+); Xref=Rhea:RHEA:31179, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62558, CC ChEBI:CHEBI:62615; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31180; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + CC H(+); Xref=Rhea:RHEA:31187, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:62548, ChEBI:CHEBI:62616; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31188; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH + CC H(+); Xref=Rhea:RHEA:31195, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:62617, ChEBI:CHEBI:62619; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31196; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxyhexanoyl-CoA + NAD(+) = 3-oxohexanoyl-CoA + NADH + CC H(+); Xref=Rhea:RHEA:31143, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:62075, ChEBI:CHEBI:62418; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31144; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(11Z)-eicosenoyl-CoA + NAD(+) = 3-oxo-(11Z)- CC eicosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83079, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:74011, ChEBI:CHEBI:232717; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83080; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z-octadecenoyl)-CoA + NAD(+) = 3-oxo-(9Z- CC octadecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:47428, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:87687, ChEBI:CHEBI:87695; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47429; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z-hexadecenoyl)-CoA + NAD(+) = 3-oxo-(7Z- CC hexadecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:47440, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:87718, ChEBI:CHEBI:87719; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47441; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(5Z-tetradecenoyl)-CoA + NAD(+) = 3-oxo-(5Z- CC tetradecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:47456, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:87705, ChEBI:CHEBI:87707; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47457; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(11Z)-octadecenoyl-CoA + NAD(+) = 3-oxo-(11Z)- CC octadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83031, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:76555, ChEBI:CHEBI:232532; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83032; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z-hexadecenoyl)-CoA + NAD(+) = 3-oxo-(9Z- CC hexadecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:82931, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232641, ChEBI:CHEBI:232642; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:82932; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z)-tetradecenoyl-CoA + NAD(+) = 3-oxo-(7Z)- CC tetradecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:82999, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:231719, ChEBI:CHEBI:232679; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83000; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(5Z)-dodecenoyl-CoA + NAD(+) = 3-oxo-(5Z)- CC dodecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83003, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232686, ChEBI:CHEBI:232696; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83004; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z)-hexadecenoyl-CoA + NAD(+) = 3-oxo-(6Z)- CC hexadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83139, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232762, ChEBI:CHEBI:232763; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83140; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z)-octadecenoyl-CoA + NAD(+) = 3-oxo-(6Z)- CC octadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83215, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232773, ChEBI:CHEBI:232774; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83216; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z)-eicosenoyl-CoA + NAD(+) = 3-oxo-(9Z)- CC eicosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83363, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232893, ChEBI:CHEBI:232898; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83364; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z)-octadecenoyl-CoA + NAD(+) = 3-oxo-(7Z)- CC octadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83379, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232900, ChEBI:CHEBI:232901; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83380; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(5Z)-hexadecenoyl-CoA + NAD(+) = 3-oxo-(5Z)- CC hexadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83395, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:232911, ChEBI:CHEBI:232912; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83396; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z,12Z)-octadecadienoyl-CoA + NAD(+) = 3-oxo- CC (9Z,12Z)-octadecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84427, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:74013, ChEBI:CHEBI:233824; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84428; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z,10Z)-hexadecadienoyl-CoA + NAD(+) = 3-oxo- CC (7Z,10Z)-hexadecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84411, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:233788, ChEBI:CHEBI:233826; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84412; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(8Z)-tetradecenoyl-CoA + NAD(+) = 3-oxo-(8Z)- CC tetradecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84415, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:233832, ChEBI:CHEBI:233833; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84416; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z)-dodecenoyl-CoA + NAD(+) = 3-oxo-(6Z)- CC dodecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84419, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:233836, ChEBI:CHEBI:233837; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84420; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(9Z,12Z,15Z)-octadecatrienoyl-CoA + NAD(+) = 3- CC oxo-(9Z,12Z,15Z)-octadecatrienoyl-CoA + NADH + H(+); CC Xref=Rhea:RHEA:84671, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:233841, ChEBI:CHEBI:233842; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84672; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z,10Z,13Z)-hexadecatrienoyl-CoA + NAD(+) = 3- CC oxo-(7Z,10Z,13Z)-hexadecatrienoyl-CoA + NADH + H(+); CC Xref=Rhea:RHEA:84675, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:233794, ChEBI:CHEBI:233843; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84676; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(8Z,11Z)-tetradecadienoyl-CoA + NAD(+) = 3-oxo- CC (8Z,11Z)-tetradecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84679, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:233803, ChEBI:CHEBI:233844; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84680; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z,9Z)-dodecadienoyl-CoA + NAD(+) = 3-oxo- CC (6Z,9Z)-dodecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84683, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, CC ChEBI:CHEBI:233848, ChEBI:CHEBI:233849; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84684; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(7Z)-decenoyl-CoA + NAD(+) = 3-oxo-(7Z)-decenoyl- CC CoA + NADH + H(+); Xref=Rhea:RHEA:84687, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:233854, CC ChEBI:CHEBI:233855; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84688; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z,9Z,12Z)-octadecatrienoyl-CoA + NAD(+) = 3- CC oxo-(6Z,9Z,12Z)-octadecatrienoyl-CoA + NADH + H(+); CC Xref=Rhea:RHEA:84691, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:233780, ChEBI:CHEBI:233839; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84692; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3S)-hydroxy-(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + NAD(+) = CC 3-oxo-(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + NADH + H(+); CC Xref=Rhea:RHEA:85131, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57945, ChEBI:CHEBI:233746, ChEBI:CHEBI:234230; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:85132; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'- CC [1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CC hexadecanoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3- CC phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-hexadecanoyl-sn-glycero-3- CC phospho]-glycerol + CoA; Xref=Rhea:RHEA:43680, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57379, ChEBI:CHEBI:83580, ChEBI:CHEBI:83583; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43681; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'- CC [1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + (9Z)- CC octadecenoyl-CoA = 1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3- CC phospho]-3'-[1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-sn- CC glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:43676, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:83580, CC ChEBI:CHEBI:83582; Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43677; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1'-[1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-3'- CC [1-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CC (9Z,12Z)-octadecadienoyl-CoA = 1',3'-bis-[1,2-di-(9Z,12Z- CC octadecadienoyl)-sn-glycero-3-phospho]-glycerol + CoA; CC Xref=Rhea:RHEA:43672, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383, CC ChEBI:CHEBI:83580, ChEBI:CHEBI:83581; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43673; CC Evidence={ECO:0000250|UniProtKB:P40939}; CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation. CC {ECO:0000250|UniProtKB:P40939}. CC -!- SUBUNIT: Heterotetramer of 2 alpha/HADHA and 2 beta/HADHB subunits; CC forms the mitochondrial trifunctional enzyme (By similarity). Also CC purified as higher order heterooligomers including a 4 alpha/HADHA and CC 4 beta/HADHB heterooligomer which physiological significance remains CC unclear (By similarity). The mitochondrial trifunctional enzyme CC interacts with MTLN (PubMed:29949755). {ECO:0000250|UniProtKB:P40939, CC ECO:0000269|PubMed:29949755}. CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:P40939}. CC Mitochondrion inner membrane {ECO:0000250|UniProtKB:P40939}. CC Note=Protein stability and association with mitochondrion inner CC membrane do not require HADHB. {ECO:0000250|UniProtKB:P40939}. CC -!- PTM: Acetylation of Lys-569 and Lys-728 is observed in liver CC mitochondria from fasted mice but not from fed mice. CC -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA CC hydratase/isomerase family. {ECO:0000305}. CC -!- SIMILARITY: In the central section; belongs to the 3-hydroxyacyl-CoA CC dehydrogenase family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK029017; BAC26245.1; -; mRNA. DR EMBL; AK170478; BAE41822.1; -; mRNA. DR EMBL; AK170683; BAE41956.1; -; mRNA. DR EMBL; BC027156; AAH27156.1; -; mRNA. DR EMBL; BC037009; AAH37009.1; -; mRNA. DR EMBL; BC046978; AAH46978.1; -; mRNA. DR EMBL; BC058569; AAH58569.1; -; mRNA. DR CCDS; CCDS19155.1; -. DR RefSeq; NP_849209.1; NM_178878.3. DR AlphaFoldDB; Q8BMS1; -. DR SMR; Q8BMS1; -. DR BioGRID; 220648; 81. DR FunCoup; Q8BMS1; 2006. DR IntAct; Q8BMS1; 8. DR STRING; 10090.ENSMUSP00000120976; -. DR GlyCosmos; Q8BMS1; 1 site, No reported glycans. DR GlyGen; Q8BMS1; 2 sites, 1 O-linked glycan (1 site). DR iPTMnet; Q8BMS1; -. DR MetOSite; Q8BMS1; -. DR PhosphoSitePlus; Q8BMS1; -. DR SwissPalm; Q8BMS1; -. DR REPRODUCTION-2DPAGE; IPI00223092; -. DR jPOST; Q8BMS1; -. DR PaxDb; 10090-ENSMUSP00000120976; -. DR PeptideAtlas; Q8BMS1; -. DR ProteomicsDB; 277753; -. DR Pumba; Q8BMS1; -. DR Antibodypedia; 3074; 272 antibodies from 36 providers. DR DNASU; 97212; -. DR Ensembl; ENSMPUT00000008617; ENSMPUP00000008481; ENSMPUG00000008546. DR Ensembl; ENSMUST00000156859.3; ENSMUSP00000120976.2; ENSMUSG00000025745.13. DR GeneID; 97212; -. DR KEGG; mmu:97212; -. DR UCSC; uc008wvc.1; mouse. DR AGR; MGI:2135593; -. DR CTD; 3030; -. DR MGI; MGI:2135593; Hadha. DR VEuPathDB; HostDB:ENSMUSG00000025745; -. DR eggNOG; KOG1683; Eukaryota. DR GeneTree; ENSGT00940000154677; -. DR HOGENOM; CLU_009834_16_1_1; -. DR InParanoid; Q8BMS1; -. DR OMA; AACHHAQ; -. DR OrthoDB; 10004768at2759; -. DR PhylomeDB; Q8BMS1; -. DR Reactome; R-MMU-1482798; Acyl chain remodeling of CL. DR Reactome; R-MMU-77285; Beta oxidation of myristoyl-CoA to lauroyl-CoA. DR Reactome; R-MMU-77305; Beta oxidation of palmitoyl-CoA to myristoyl-CoA. DR Reactome; R-MMU-77310; Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA. DR Reactome; R-MMU-77346; Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA. DR Reactome; R-MMU-77348; Beta oxidation of octanoyl-CoA to hexanoyl-CoA. DR Reactome; R-MMU-77350; Beta oxidation of hexanoyl-CoA to butanoyl-CoA. DR UniPathway; UPA00659; -. DR BioGRID-ORCS; 97212; 4 hits in 79 CRISPR screens. DR CD-CODE; CE726F99; Postsynaptic density. DR PRO; PR:Q8BMS1; -. DR Proteomes; UP000000589; Chromosome 5. DR RNAct; Q8BMS1; protein. DR Bgee; ENSMUSG00000025745; Expressed in myocardium of ventricle and 250 other cell types or tissues. DR GO; GO:0016507; C:mitochondrial fatty acid beta-oxidation multienzyme complex; ISO:GO_Central. DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI. DR GO; GO:0042645; C:mitochondrial nucleoid; ISO:GO_Central. DR GO; GO:0005739; C:mitochondrion; HDA:MGI. DR GO; GO:0003857; F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity; ISO:GO_Central. DR GO; GO:0018812; F:3-hydroxyacyl-CoA dehydratase activity; ISS:UniProtKB. DR GO; GO:0003988; F:acetyl-CoA C-acyltransferase activity; ISO:GO_Central. DR GO; GO:0004300; F:enoyl-CoA hydratase activity; ISS:UniProtKB. DR GO; GO:0000062; F:fatty-acyl-CoA binding; ISO:GO_Central. DR GO; GO:0016509; F:long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity; IDA:MGI. DR GO; GO:0052816; F:long-chain fatty acyl-CoA hydrolase activity; ISO:GO_Central. DR GO; GO:0051287; F:NAD binding; ISO:GO_Central. DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro. DR GO; GO:0044877; F:protein-containing complex binding; ISO:GO_Central. DR GO; GO:0035965; P:cardiolipin acyl-chain remodeling; ISS:UniProtKB. DR GO; GO:0006635; P:fatty acid beta-oxidation; IMP:MGI. DR GO; GO:0032868; P:response to insulin; IMP:MGI. DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:GO_Central. DR CDD; cd06558; crotonase-like; 1. DR FunFam; 3.90.226.10:FF:000011; Fatty acid oxidation complex subunit alpha; 1. DR FunFam; 3.40.50.720:FF:000009; Fatty oxidation complex, alpha subunit; 1. DR FunFam; 1.10.1040.50:FF:000002; Trifunctional enzyme subunit alpha, mitochondrial; 1. DR Gene3D; 1.10.1040.50; -; 1. DR Gene3D; 3.90.226.10; 2-enoyl-CoA Hydratase, Chain A, domain 1; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR InterPro; IPR006180; 3-OHacyl-CoA_DH_CS. DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd. DR InterPro; IPR006108; 3HC_DH_C. DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf. DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf. DR InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS. DR InterPro; IPR001753; Enoyl-CoA_hydra/iso. DR InterPro; IPR012803; Fa_ox_alpha_mit. DR InterPro; IPR050136; FA_oxidation_alpha_subunit. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR NCBIfam; TIGR02441; fa_ox_alpha_mit; 1. DR PANTHER; PTHR43612; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA; 1. DR PANTHER; PTHR43612:SF3; TRIFUNCTIONAL ENZYME SUBUNIT ALPHA, MITOCHONDRIAL; 1. DR Pfam; PF00725; 3HCDH; 2. DR Pfam; PF02737; 3HCDH_N; 1. DR Pfam; PF00378; ECH_1; 1. DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2. DR SUPFAM; SSF52096; ClpP/crotonase; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR PROSITE; PS00067; 3HCDH; 1. DR PROSITE; PS00166; ENOYL_COA_HYDRATASE; 1. PE 1: Evidence at protein level; KW Acetylation; Fatty acid metabolism; Lipid metabolism; Lyase; Membrane; KW Methylation; Mitochondrion; Mitochondrion inner membrane; KW Multifunctional enzyme; NAD; Oxidoreductase; Phosphoprotein; KW Reference proteome; Transferase; Transit peptide. FT TRANSIT 1..36 FT /note="Mitochondrion" FT /evidence="ECO:0000255" FT CHAIN 37..763 FT /note="Trifunctional enzyme subunit alpha, mitochondrial" FT /id="PRO_0000322639" FT ACT_SITE 510 FT /note="For hydroxyacyl-coenzyme A dehydrogenase activity" FT /evidence="ECO:0000250|UniProtKB:P40939" FT SITE 151 FT /note="Important for long-chain enoyl-CoA hydratase FT activity" FT /evidence="ECO:0000250|UniProtKB:P40939" FT SITE 173 FT /note="Important for long-chain enoyl-CoA hydratase FT activity" FT /evidence="ECO:0000250|UniProtKB:P40939" FT SITE 498 FT /note="Important for hydroxyacyl-coenzyme A dehydrogenase FT activity" FT /evidence="ECO:0000250|UniProtKB:P40939" FT MOD_RES 46 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 46 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 60 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753, FT ECO:0007744|PubMed:23806337" FT MOD_RES 60 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 129 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:16916647, FT ECO:0007744|PubMed:23576753" FT MOD_RES 166 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 166 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 213 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 214 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 214 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 230 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 231 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 249 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 249 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 289 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 295 FT /note="N6-acetyllysine" FT /evidence="ECO:0000250|UniProtKB:P40939" FT MOD_RES 303 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 303 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 316 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 326 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 326 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 334 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 334 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 350 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 350 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 353 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 395 FT /note="Phosphothreonine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 399 FT /note="Omega-N-methylarginine" FT /evidence="ECO:0007744|PubMed:24129315" FT MOD_RES 406 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753, FT ECO:0007744|PubMed:23806337" FT MOD_RES 406 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 411 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 411 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 415 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 419 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q64428" FT MOD_RES 436 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 436 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 440 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 460 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 460 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 505 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 505 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 519 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 519 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 540 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 569 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 569 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 620 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 634 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 644 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 644 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 646 FT /note="N6-succinyllysine" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 647 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" FT MOD_RES 650 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q64428" FT MOD_RES 664 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 664 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 728 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 728 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT MOD_RES 735 FT /note="N6-acetyllysine" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 759 FT /note="N6-acetyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23576753" FT MOD_RES 759 FT /note="N6-succinyllysine; alternate" FT /evidence="ECO:0007744|PubMed:23806337" FT CONFLICT 196 FT /note="A -> D (in Ref. 1; BAE41822)" FT /evidence="ECO:0000305" FT CONFLICT 459 FT /note="L -> S (in Ref. 2; AAH37009)" FT /evidence="ECO:0000305" SQ SEQUENCE 763 AA; 82670 MW; 73D203795D5C1141 CRC64; MVASRAIGSL SRFSAFRILR SRGCICRSFT TSSALLTRTH INYGVKGDVA VIRINSPNSK VNTLNKEVQS EFIEVMNEIW ANDQIRSAVL ISSKPGCFVA GADINMLSSC TTPQEATRIS QEGQRMFEKL EKSPKPVVAA ISGSCLGGGL ELAIACQYRI ATKDRKTVLG VPEVLLGILP GAGGTQRLPK MVGVPAAFDM MLTGRNIRAD RAKKMGLVDQ LVEPLGPGIK SPEERTIEYL EEVAVNFAKG LADRKVSAKQ SKGLVEKLTT YAMTVPFVRQ QVYKTVEEKV KKQTKGLYPA PLKIIDAVKA GLEQGSDAGY LAESQKFGEL ALTKESKALM GLYNGQVLCK KNKFGAPQKN VQQLAILGAG LMGAGIAQVS VDKGLKTLLK DTTVTGLGRG QQQVFKGLND KVKKKALTSF ERDSIFSNLI GQLDYKGFEK ADMVIEAVFE DLGVKHKVLK EVESVTPEHC IFASNTSALP INQIAAVSKR PEKVIGMHYF SPVDKMQLLE IITTDKTSKD TTASAVAVGL RQGKVIIVVK DGPGFYTTRC LAPMMSEVMR ILQEGVDPKK LDALTTGFGF PVGAATLADE VGVDVAQHVA EDLGKAFGER FGGGSVELLK QMVSKGFLGR KSGKGFYIYQ EGSKNKSLNS EMDNILANLR LPAKPEVSSD EDVQYRVITR FVNEAVLCLQ EGILATPAEG DIGAVFGLGF PPCLGGPFRF VDLYGAQKVV DRLRKYESAY GTQFTPCQLL LDHANNSSKK FYQ //