ID   Q8AXS8_XENLA            Unreviewed;       380 AA.
AC   Q8AXS8;
DT   01-MAR-2003, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2003, sequence version 1.
DT   02-SEP-2026, entry version 54.
DE   RecName: Full=GDP-fucose protein O-fucosyltransferase 1 {ECO:0000256|ARBA:ARBA00021745};
DE            EC=2.4.1.221 {ECO:0000256|ARBA:ARBA00012196};
DE   AltName: Full=Peptide-O-fucosyltransferase 1 {ECO:0000256|ARBA:ARBA00033080};
GN   Name=pofut1 {ECO:0000313|EMBL:CAD55833.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355 {ECO:0000313|EMBL:CAD55833.1};
RN   [1] {ECO:0000313|EMBL:CAD55833.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Oriol R.;
RL   Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-
CC         L-seryl-[protein] + GDP + H(+); Xref=Rhea:RHEA:63644, Rhea:RHEA-
CC         COMP:9863, Rhea:RHEA-COMP:17914, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29999, ChEBI:CHEBI:57273, ChEBI:CHEBI:58189,
CC         ChEBI:CHEBI:189632; EC=2.4.1.221;
CC         Evidence={ECO:0000256|ARBA:ARBA00048647};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63645;
CC         Evidence={ECO:0000256|ARBA:ARBA00048647};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-
CC         fucosyl)-L-threonyl-[protein] + GDP + H(+); Xref=Rhea:RHEA:70491,
CC         Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:17915, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30013, ChEBI:CHEBI:57273, ChEBI:CHEBI:58189,
CC         ChEBI:CHEBI:189631; EC=2.4.1.221;
CC         Evidence={ECO:0000256|ARBA:ARBA00047273};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70492;
CC         Evidence={ECO:0000256|ARBA:ARBA00047273};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|ARBA:ARBA00004922}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000256|ARBA:ARBA00004240}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 65 family.
CC       {ECO:0000256|ARBA:ARBA00010626}.
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DR   EMBL; AJ514425; CAD55833.1; -; mRNA.
DR   AlphaFoldDB; Q8AXS8; -.
DR   UniPathway; UPA00378; -.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0046922; F:peptide-O-fucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0007219; P:Notch signaling pathway; IEA:InterPro.
DR   GO; GO:0036066; P:protein O-linked glycosylation via fucose; IEA:InterPro.
DR   GO; GO:0008593; P:regulation of Notch signaling pathway; IEA:TreeGrafter.
DR   CDD; cd11302; O-FucT-1; 1.
DR   FunFam; 3.40.50.11340:FF:000001; GDP-fucose protein O-fucosyltransferase 1; 1.
DR   FunFam; 3.40.50.11350:FF:000004; GDP-fucose protein O-fucosyltransferase 1; 1.
DR   Gene3D; 3.40.50.11340; -; 1.
DR   Gene3D; 3.40.50.11350; -; 1.
DR   InterPro; IPR019378; GDP-Fuc_O-FucTrfase.
DR   InterPro; IPR039922; POFUT1.
DR   PANTHER; PTHR21420; GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1; 1.
DR   PANTHER; PTHR21420:SF3; GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1; 1.
DR   Pfam; PF10250; O-FucT; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW   Fucose metabolism {ECO:0000256|ARBA:ARBA00023253};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW   Notch signaling pathway {ECO:0000256|ARBA:ARBA00022976};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           20..380
FT                   /note="GDP-fucose protein O-fucosyltransferase 1"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004305629"
SQ   SEQUENCE   380 AA;  43505 MW;  C6B960E76D875293 CRC64;
     MERGVWLFLL LLPFVGCEGL YNSNGYIVYC PCMGRFGNQA DHFLGSLAFA KMVNRTLVVP
     PWIVYNHHRP PYTNVHVPYE EFFQLEPLRQ FHQVISMEEF MEQLAPVLWP PTKRLAYCFT
     AAAQRSPDKK SCPMKDGNPF GPFWDHFHVD FTQSELFDGI TFSAYYKDVW ISRFPPSQHP
     VIALPGAPAQ FPVLEEHRSL QRYIVWSEKI VREGEEQIRS LLVRPYVGIH LRIGSDWKDA
     CKMLKDGTAG SHVMASAQCV CYDRYRAGPL TMDMCLPDLK EMRRALTLWV ERSKARSVYI
     ATDSPSYTAE IQAFLGNEVR VVCLQPEVAQ IDLYILAQAD HFIGNCVSSF TAFVKRDRDV
     HGRPSSFFGM DSPGVVQDEL
//