ID   GLGB_BRADU              Reviewed;         721 AA.
AC   Q89FD3;
DT   15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   02-SEP-2026, entry version 138.
DE   RecName: Full=1,4-alpha-glucan branching enzyme GlgB {ECO:0000255|HAMAP-Rule:MF_00685};
DE            EC=2.4.1.18 {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=Alpha-(1->4)-glucan branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE   AltName: Full=Glycogen branching enzyme {ECO:0000255|HAMAP-Rule:MF_00685};
DE            Short=BE {ECO:0000255|HAMAP-Rule:MF_00685};
GN   Name=glgB {ECO:0000255|HAMAP-Rule:MF_00685};
GN   OrderedLocusNames=blr6768;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Nitrobacteraceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC       in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC       growing alpha-1,4-glucan chains and the subsequent attachment of the
CC       oligosaccharide to the alpha-1,6 position. {ECO:0000255|HAMAP-
CC       Rule:MF_00685}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC         primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00685};
CC   -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00685}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00685}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00685}.
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DR   EMBL; BA000040; BAC52033.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q89FD3; -.
DR   SMR; Q89FD3; -.
DR   FunCoup; Q89FD3; 601.
DR   STRING; 224911.AAV28_31440; -.
DR   PaxDb; 224911-AAV28_31440; -.
DR   EnsemblBacteria; BAC52033; BAC52033; BAC52033.
DR   KEGG; bja:blr6768; -.
DR   PATRIC; fig|224911.5.peg.6935; -.
DR   eggNOG; COG0296; Bacteria.
DR   HOGENOM; CLU_004245_3_2_5; -.
DR   InParanoid; Q89FD3; -.
DR   OrthoDB; 9800174at2; -.
DR   PhylomeDB; Q89FD3; -.
DR   UniPathway; UPA00164; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005978; P:glycogen biosynthetic process; IBA:GO_Central.
DR   CDD; cd11322; AmyAc_Glg_BE; 1.
DR   CDD; cd02855; E_set_GBE_prok_N; 1.
DR   FunFam; 2.60.40.10:FF:000169; 1,4-alpha-glucan branching enzyme GlgB; 1.
DR   FunFam; 2.60.40.1180:FF:000002; 1,4-alpha-glucan branching enzyme GlgB; 1.
DR   FunFam; 3.20.20.80:FF:000003; 1,4-alpha-glucan branching enzyme GlgB; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   HAMAP; MF_00685; GlgB; 1.
DR   InterPro; IPR006048; A-amylase/branching_C.
DR   InterPro; IPR037439; Branching_enzy.
DR   InterPro; IPR006047; GH13_cat_dom.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR006407; GlgB.
DR   InterPro; IPR054169; GlgB_N.
DR   InterPro; IPR044143; GlgB_N_E_set_prok.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR01515; branching_enzym; 1.
DR   NCBIfam; NF003811; PRK05402.1; 1.
DR   NCBIfam; NF008967; PRK12313.1; 1.
DR   PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR   PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02806; Alpha-amylase_C; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF22019; GlgB_N; 1.
DR   PIRSF; PIRSF000463; GlgB; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Glycogen biosynthesis; Glycogen metabolism;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..721
FT                   /note="1,4-alpha-glucan branching enzyme GlgB"
FT                   /id="PRO_0000188688"
FT   ACT_SITE        404
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
FT   ACT_SITE        457
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00685"
SQ   SEQUENCE   721 AA;  81677 MW;  41622A1E97207F87 CRC64;
     MPRAKPMPKL PAEAYAIIEG RHADPFHYLG LHPEGGKSVV RAFLPEASNV EAVGEHGEVA
     RLDRVHDAGL FVGALPNGSK HYQLRAKFGD SIVEFEDAYR FPPILTDFDL YLLGEGTHQR
     LYDKLGAHPM TLDGVDGIGF VVLAPNARRV SVVGDFNFWD SRRHPMRVRG SGYWELFIPH
     AKRGDHYKFD IVGPHGHQLP LKSDPMAFAS EVRPKTASIV FDEAHLPRPR PAPDGINALS
     APMSIYEVHL GSWRRKGDNE WLTYRELAEQ LPAYARDMGF THLEFLPVSE HPFDGSWGYQ
     PTGLYAPTSR FGTPEDFAAL VDACHREGVG VLLDWVPGHF PDDPHGLGSF DGTSLYEHAN
     PLQGRHLDWG TLIYNYGRTE VTNFLVSNAL FWLERYAIDG LRVDAVASML YLDYSRPPGA
     WIPNQYGGRE NIEAIAFLRR FNTELFARFP QATTAAEEST AWPQVSRPVE FGGLGFGYKW
     NMGWMHDTLN YISKDPIHRK HHHGEILFGL HYAFSENFIL PLSHDEVVHG KRSILGRMPG
     DEWQRFANLR AYYSFMFGHP GKKLLFMGAE IAQSREWNHD QSLDWHLLEY KYHSGIQALI
     RDLNRLYRAV PALHQMDCDQ AGFEWLITDD ANRNVFAWLR KGLDEHARCL VIVNFSPNVY
     RNYRVRVPFA GKWKEVLNSD SAHYGGSNVG NIGEVHAVDG KTPELRLTIP PLAAIFLVPE
     S
//