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<entry dataset="Swiss-Prot" created="2005-02-01" modified="2026-01-28" version="103" xmlns="http://uniprot.org/uniprot">
  <accession>Q88QT9</accession>
  <name>GLPE_PSEPK</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Thiosulfate sulfurtransferase GlpE</fullName>
      <ecNumber evidence="1">2.8.1.1</ecNumber>
    </recommendedName>
  </protein>
  <gene>
    <name evidence="1" type="primary">glpE</name>
    <name type="ordered locus">PP_0398</name>
  </gene>
  <organism>
    <name type="scientific">Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)</name>
    <dbReference type="NCBI Taxonomy" id="160488"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Pseudomonadales</taxon>
      <taxon>Pseudomonadaceae</taxon>
      <taxon>Pseudomonas</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2002" name="Environ. Microbiol." volume="4" first="799" last="808">
      <title>Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.</title>
      <authorList>
        <person name="Nelson K.E."/>
        <person name="Weinel C."/>
        <person name="Paulsen I.T."/>
        <person name="Dodson R.J."/>
        <person name="Hilbert H."/>
        <person name="Martins dos Santos V.A.P."/>
        <person name="Fouts D.E."/>
        <person name="Gill S.R."/>
        <person name="Pop M."/>
        <person name="Holmes M."/>
        <person name="Brinkac L.M."/>
        <person name="Beanan M.J."/>
        <person name="DeBoy R.T."/>
        <person name="Daugherty S.C."/>
        <person name="Kolonay J.F."/>
        <person name="Madupu R."/>
        <person name="Nelson W.C."/>
        <person name="White O."/>
        <person name="Peterson J.D."/>
        <person name="Khouri H.M."/>
        <person name="Hance I."/>
        <person name="Chris Lee P."/>
        <person name="Holtzapple E.K."/>
        <person name="Scanlan D."/>
        <person name="Tran K."/>
        <person name="Moazzez A."/>
        <person name="Utterback T.R."/>
        <person name="Rizzo M."/>
        <person name="Lee K."/>
        <person name="Kosack D."/>
        <person name="Moestl D."/>
        <person name="Wedler H."/>
        <person name="Lauber J."/>
        <person name="Stjepandic D."/>
        <person name="Hoheisel J."/>
        <person name="Straetz M."/>
        <person name="Heim S."/>
        <person name="Kiewitz C."/>
        <person name="Eisen J.A."/>
        <person name="Timmis K.N."/>
        <person name="Duesterhoeft A."/>
        <person name="Tuemmler B."/>
        <person name="Fraser C.M."/>
      </authorList>
      <dbReference type="PubMed" id="12534463"/>
      <dbReference type="DOI" id="10.1046/j.1462-2920.2002.00366.x"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Transferase that catalyzes the transfer of sulfur from thiosulfate to thiophilic acceptors such as cyanide or dithiols. May function in a CysM-independent thiosulfate assimilation pathway by catalyzing the conversion of thiosulfate to sulfite, which can then be used for L-cysteine biosynthesis.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>thiosulfate + hydrogen cyanide = thiocyanate + sulfite + 2 H(+)</text>
      <dbReference type="Rhea" id="RHEA:16881"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:17359"/>
      <dbReference type="ChEBI" id="CHEBI:18022"/>
      <dbReference type="ChEBI" id="CHEBI:18407"/>
      <dbReference type="ChEBI" id="CHEBI:33542"/>
      <dbReference type="EC" id="2.8.1.1"/>
    </reaction>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>thiosulfate + [thioredoxin]-dithiol = [thioredoxin]-disulfide + hydrogen sulfide + sulfite + 2 H(+)</text>
      <dbReference type="Rhea" id="RHEA:83859"/>
      <dbReference type="Rhea" id="RHEA-COMP:10698"/>
      <dbReference type="Rhea" id="RHEA-COMP:10700"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:17359"/>
      <dbReference type="ChEBI" id="CHEBI:29919"/>
      <dbReference type="ChEBI" id="CHEBI:29950"/>
      <dbReference type="ChEBI" id="CHEBI:33542"/>
      <dbReference type="ChEBI" id="CHEBI:50058"/>
    </reaction>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the GlpE family.</text>
  </comment>
  <dbReference type="EC" id="2.8.1.1" evidence="1"/>
  <dbReference type="EMBL" id="AE015451">
    <property type="protein sequence ID" value="AAN66029.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_010951742.1">
    <property type="nucleotide sequence ID" value="NC_002947.4"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q88QT9"/>
  <dbReference type="SMR" id="Q88QT9"/>
  <dbReference type="STRING" id="160488.PP_0398"/>
  <dbReference type="PaxDb" id="160488-PP_0398"/>
  <dbReference type="GeneID" id="83677689"/>
  <dbReference type="KEGG" id="ppu:PP_0398"/>
  <dbReference type="PATRIC" id="fig|160488.4.peg.428"/>
  <dbReference type="eggNOG" id="COG0607">
    <property type="taxonomic scope" value="Bacteria"/>
  </dbReference>
  <dbReference type="HOGENOM" id="CLU_089574_14_0_6"/>
  <dbReference type="OrthoDB" id="9811849at2"/>
  <dbReference type="PhylomeDB" id="Q88QT9"/>
  <dbReference type="BioCyc" id="PPUT160488:G1G01-435-MONOMER"/>
  <dbReference type="Proteomes" id="UP000000556">
    <property type="component" value="Chromosome"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005737">
    <property type="term" value="C:cytoplasm"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-SubCell"/>
  </dbReference>
  <dbReference type="GO" id="GO:0004792">
    <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0103041">
    <property type="term" value="F:thiosulfate-thioredoxin sulfurtransferase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="RHEA"/>
  </dbReference>
  <dbReference type="CDD" id="cd01444">
    <property type="entry name" value="GlpE_ST"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.40.250.10">
    <property type="entry name" value="Rhodanese-like domain"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_01009">
    <property type="entry name" value="Thiosulf_sulfurtr"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR050229">
    <property type="entry name" value="GlpE_sulfurtransferase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR001763">
    <property type="entry name" value="Rhodanese-like_dom"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR036873">
    <property type="entry name" value="Rhodanese-like_dom_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR023695">
    <property type="entry name" value="Thiosulf_sulfurTrfase"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF001195">
    <property type="entry name" value="PRK00162.1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43031">
    <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43031:SF6">
    <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE GLPE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00581">
    <property type="entry name" value="Rhodanese"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SMART" id="SM00450">
    <property type="entry name" value="RHOD"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF52821">
    <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS50206">
    <property type="entry name" value="RHODANESE_3"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-1185">Reference proteome</keyword>
  <keyword id="KW-0808">Transferase</keyword>
  <feature type="chain" id="PRO_0000200560" description="Thiosulfate sulfurtransferase GlpE">
    <location>
      <begin position="1"/>
      <end position="110"/>
    </location>
  </feature>
  <feature type="domain" description="Rhodanese" evidence="1">
    <location>
      <begin position="17"/>
      <end position="105"/>
    </location>
  </feature>
  <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
    <location>
      <position position="65"/>
    </location>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_01009"/>
    </source>
  </evidence>
  <sequence length="110" mass="11906" checksum="295FA59984EBDBF3" modified="2003-06-01" version="1">MSEFKRIPPEQALELRKKEGAVVVDIRDPQAFAAGHITGARHLDNHSVADFIRNADLDAPTLVVCYHGNSSQSAAAYLVGQGFSDVYSVDGGFELWRATYPAETAQGNAE</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>