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<entry dataset="TrEMBL" created="2003-10-01" modified="2026-11-04" version="53" xmlns="http://uniprot.org/uniprot">
  <accession>Q7Y1T9</accession>
  <name>Q7Y1T9_BRANA</name>
  <protein>
    <recommendedName>
      <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
      <ecNumber evidence="3">3.2.1.39</ecNumber>
    </recommendedName>
  </protein>
  <gene>
    <name evidence="13" type="primary">Bger1</name>
  </gene>
  <organism evidence="13">
    <name type="scientific">Brassica napus</name>
    <name type="common">Rape</name>
    <dbReference type="NCBI Taxonomy" id="3708"/>
    <lineage>
      <taxon>Eukaryota</taxon>
      <taxon>Viridiplantae</taxon>
      <taxon>Streptophyta</taxon>
      <taxon>Embryophyta</taxon>
      <taxon>Tracheophyta</taxon>
      <taxon>Spermatophyta</taxon>
      <taxon>Magnoliopsida</taxon>
      <taxon>eudicotyledons</taxon>
      <taxon>Gunneridae</taxon>
      <taxon>Pentapetalae</taxon>
      <taxon>rosids</taxon>
      <taxon>malvids</taxon>
      <taxon>Brassicales</taxon>
      <taxon>Brassicaceae</taxon>
      <taxon>Brassiceae</taxon>
      <taxon>Brassica</taxon>
    </lineage>
  </organism>
  <reference evidence="13" key="1">
    <citation type="journal article" date="1998" name="Plant Mol. Biol." volume="37" first="763" last="772">
      <title>Conserved structure and function of the Arabidopsis flowering time gene CONSTANS in Brassica napus.</title>
      <authorList>
        <person name="Robert L.S."/>
        <person name="Robson F."/>
        <person name="Sharpe A."/>
        <person name="Lydiate D."/>
        <person name="Coupland G."/>
      </authorList>
      <dbReference type="PubMed" id="9678571"/>
      <dbReference type="DOI" id="10.1023/A:1006064514311"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE</scope>
    <source>
      <strain evidence="13">N-o-1</strain>
    </source>
  </reference>
  <reference evidence="13" key="2">
    <citation type="submission" date="2006-01" db="EMBL/GenBank/DDBJ databases">
      <title>Characterization of Brassica napus genomic clone Bn101Co1 containing Constans, Constans-like and Beta-glucan elicitor receptor genes.</title>
      <authorList>
        <person name="Robert L.S."/>
        <person name="Lydiate D."/>
        <person name="Coupland G."/>
      </authorList>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE</scope>
    <source>
      <strain evidence="13">N-o-1</strain>
    </source>
  </reference>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
      <dbReference type="EC" id="3.2.1.39"/>
    </reaction>
  </comment>
  <comment type="similarity">
    <text evidence="2 9">Belongs to the glycosyl hydrolase 81 family.</text>
  </comment>
  <comment type="caution">
    <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
  </comment>
  <dbReference type="EC" id="3.2.1.39" evidence="3"/>
  <dbReference type="EMBL" id="AY280868">
    <property type="protein sequence ID" value="AAP42646.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q7Y1T9"/>
  <dbReference type="GO" id="GO:0042973">
    <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="Gene3D" id="2.70.98.30">
    <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR005200">
    <property type="entry name" value="Endo-beta-glucanase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR040720">
    <property type="entry name" value="GH81_C"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR040451">
    <property type="entry name" value="GH81_N"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR31983">
    <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR31983:SF0">
    <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 2"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF17652">
    <property type="entry name" value="Glyco_hydro81C"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF03639">
    <property type="entry name" value="Glyco_hydro_81"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS52008">
    <property type="entry name" value="GH81"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword evidence="5 9" id="KW-0119">Carbohydrate metabolism</keyword>
  <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
  <keyword evidence="6 9" id="KW-0326">Glycosidase</keyword>
  <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
  <keyword evidence="8 9" id="KW-0624">Polysaccharide degradation</keyword>
  <keyword evidence="13" id="KW-0675">Receptor</keyword>
  <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="11">
    <location>
      <begin position="81"/>
      <end position="354"/>
    </location>
  </feature>
  <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="12">
    <location>
      <begin position="362"/>
      <end position="718"/>
    </location>
  </feature>
  <feature type="region of interest" description="Disordered" evidence="10">
    <location>
      <begin position="1"/>
      <end position="54"/>
    </location>
  </feature>
  <feature type="region of interest" description="beta-sandwich subdomain" evidence="9">
    <location>
      <begin position="47"/>
      <end position="259"/>
    </location>
  </feature>
  <feature type="region of interest" description="(alpha/beta)6 barrel subdomain" evidence="9">
    <location>
      <begin position="360"/>
      <end position="722"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Basic residues" evidence="10">
    <location>
      <begin position="1"/>
      <end position="25"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Pro residues" evidence="10">
    <location>
      <begin position="26"/>
      <end position="47"/>
    </location>
  </feature>
  <feature type="active site" evidence="9">
    <location>
      <position position="469"/>
    </location>
  </feature>
  <feature type="active site" evidence="9">
    <location>
      <position position="551"/>
    </location>
  </feature>
  <feature type="active site" evidence="9">
    <location>
      <position position="555"/>
    </location>
  </feature>
  <evidence type="ECO:0000256" key="1">
    <source>
      <dbReference type="ARBA" id="ARBA00000382"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="2">
    <source>
      <dbReference type="ARBA" id="ARBA00010730"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="3">
    <source>
      <dbReference type="ARBA" id="ARBA00012780"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="4">
    <source>
      <dbReference type="ARBA" id="ARBA00022801"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="5">
    <source>
      <dbReference type="ARBA" id="ARBA00023277"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="6">
    <source>
      <dbReference type="ARBA" id="ARBA00023295"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="7">
    <source>
      <dbReference type="ARBA" id="ARBA00023316"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="8">
    <source>
      <dbReference type="ARBA" id="ARBA00023326"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="9">
    <source>
      <dbReference type="PROSITE-ProRule" id="PRU01352"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="10">
    <source>
      <dbReference type="SAM" id="MobiDB-lite"/>
    </source>
  </evidence>
  <evidence type="ECO:0000259" key="11">
    <source>
      <dbReference type="Pfam" id="PF03639"/>
    </source>
  </evidence>
  <evidence type="ECO:0000259" key="12">
    <source>
      <dbReference type="Pfam" id="PF17652"/>
    </source>
  </evidence>
  <evidence type="ECO:0000313" key="13">
    <source>
      <dbReference type="EMBL" id="AAP42646.1"/>
    </source>
  </evidence>
  <sequence length="752" mass="83478" checksum="BEC401E75A5E070A" modified="2003-10-01" version="1">MLKKVRRKVKAFVTKPFKKPNKTRPSRPPSPEPPPPTSPLPQSPPPQEMSSSRQKNAPFLFPRSESSVLPDPSRFFSHDLLSTPLPTNSFFQNFTLKNGDQAEYFHPYIIKPSPSSLSISYPTLSHNSAFIFEAFNADITISGSDGPDPHSRKTHLISSFSDLGVTLDFPSSNLRFFLVRGSPFITCSVSGTSSITISTIHAVLSFSGNSSSTKYTAKLNNNQTWLIYASSPVHLNQTGGSSINSGAGFSGILRFALLPDPNPDFESTLDRFSCCYPISGDADLTKPFTLEYNWEKRGYGDLLMLAHPLHLKLLSTHDCSISVLESFRYRTIDGDLVGIIGDSWVLRPDPVSVTWHSIKGVDEDRREEIISALVKDVTDLDSSAPVTNSSYFYAKLIARAARLALIAEEVCYLDVIPAIRKYLKSMIEPWLDGTFEPNGFLYDPTWGGVITKQGSRDSGADFGFGIYNDHHYHLGYFLYAIAVMAKIDPLWGKRYRPQAYALMADFMTLGKKKGASFSSNSVYPRLRCFDLFKLHSWAGGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASTVLTLEIHAAKMWWQVKEGDTIYPADFTAENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPILPVSEILFSDVKFVKQLVNWTMPALAREGVGEGWKGFVYALESIYDKDGAMEKVKRLNGHDDGNSLTNLLWWIHSRGGDDDEDDDDEGGYGGHGGGGKYCSFGHYCN</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>