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<entry dataset="Swiss-Prot" created="2006-01-24" modified="2026-09-02" version="116" xmlns="http://uniprot.org/uniprot">
  <accession>Q65QT9</accession>
  <name>PDXH_MANSM</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Pyridoxine/pyridoxamine 5'-phosphate oxidase</fullName>
      <ecNumber evidence="1">1.4.3.5</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">PNP/PMP oxidase</fullName>
      <shortName evidence="1">PNPOx</shortName>
    </alternativeName>
    <alternativeName>
      <fullName evidence="1">Pyridoxal 5'-phosphate synthase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">pdxH</name>
    <name type="ordered locus">MS2064</name>
  </gene>
  <organism>
    <name type="scientific">Mannheimia succiniciproducens (strain KCTC 0769BP / MBEL55E)</name>
    <dbReference type="NCBI Taxonomy" id="221988"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Pasteurellales</taxon>
      <taxon>Pasteurellaceae</taxon>
      <taxon>Basfia</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2004" name="Nat. Biotechnol." volume="22" first="1275" last="1281">
      <title>The genome sequence of the capnophilic rumen bacterium Mannheimia succiniciproducens.</title>
      <authorList>
        <person name="Hong S.H."/>
        <person name="Kim J.S."/>
        <person name="Lee S.Y."/>
        <person name="In Y.H."/>
        <person name="Choi S.S."/>
        <person name="Rih J.-K."/>
        <person name="Kim C.H."/>
        <person name="Jeong H."/>
        <person name="Hur C.G."/>
        <person name="Kim J.J."/>
      </authorList>
      <dbReference type="PubMed" id="15378067"/>
      <dbReference type="DOI" id="10.1038/nbt1010"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>KCTC 0769BP / MBEL55E</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>pyridoxamine 5'-phosphate + O2 + H2O = pyridoxal 5'-phosphate + H2O2 + NH4(+)</text>
      <dbReference type="Rhea" id="RHEA:15817"/>
      <dbReference type="ChEBI" id="CHEBI:15377"/>
      <dbReference type="ChEBI" id="CHEBI:15379"/>
      <dbReference type="ChEBI" id="CHEBI:16240"/>
      <dbReference type="ChEBI" id="CHEBI:28938"/>
      <dbReference type="ChEBI" id="CHEBI:58451"/>
      <dbReference type="ChEBI" id="CHEBI:597326"/>
      <dbReference type="EC" id="1.4.3.5"/>
    </reaction>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2</text>
      <dbReference type="Rhea" id="RHEA:15149"/>
      <dbReference type="ChEBI" id="CHEBI:15379"/>
      <dbReference type="ChEBI" id="CHEBI:16240"/>
      <dbReference type="ChEBI" id="CHEBI:58589"/>
      <dbReference type="ChEBI" id="CHEBI:597326"/>
      <dbReference type="EC" id="1.4.3.5"/>
    </reaction>
  </comment>
  <comment type="cofactor">
    <cofactor evidence="1">
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </cofactor>
    <text evidence="1">Binds 1 FMN per subunit.</text>
  </comment>
  <comment type="pathway">
    <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
  </comment>
  <comment type="pathway">
    <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
  </comment>
  <comment type="subunit">
    <text evidence="1">Homodimer.</text>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
  </comment>
  <dbReference type="EC" id="1.4.3.5" evidence="1"/>
  <dbReference type="EMBL" id="AE016827">
    <property type="protein sequence ID" value="AAU38671.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q65QT9"/>
  <dbReference type="SMR" id="Q65QT9"/>
  <dbReference type="STRING" id="221988.MS2064"/>
  <dbReference type="KEGG" id="msu:MS2064"/>
  <dbReference type="eggNOG" id="COG0259">
    <property type="taxonomic scope" value="Bacteria"/>
  </dbReference>
  <dbReference type="HOGENOM" id="CLU_032263_2_2_6"/>
  <dbReference type="UniPathway" id="UPA01068">
    <property type="reaction ID" value="UER00304"/>
  </dbReference>
  <dbReference type="UniPathway" id="UPA01068">
    <property type="reaction ID" value="UER00305"/>
  </dbReference>
  <dbReference type="Proteomes" id="UP000000607">
    <property type="component" value="Chromosome"/>
  </dbReference>
  <dbReference type="GO" id="GO:0010181">
    <property type="term" value="F:FMN binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0004733">
    <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0008615">
    <property type="term" value="P:pyridoxine biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="FunFam" id="2.30.110.10:FF:000014">
    <property type="entry name" value="Pyridoxine/pyridoxamine 5'-phosphate oxidase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="2.30.110.10">
    <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_01629">
    <property type="entry name" value="PdxH"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR000659">
    <property type="entry name" value="Pyridox_Oxase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR019740">
    <property type="entry name" value="Pyridox_Oxase_CS"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011576">
    <property type="entry name" value="Pyridox_Oxase_N"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR019576">
    <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR012349">
    <property type="entry name" value="Split_barrel_FMN-bd"/>
  </dbReference>
  <dbReference type="NCBIfam" id="TIGR00558">
    <property type="entry name" value="pdxH"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF004231">
    <property type="entry name" value="PRK05679.1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR10851:SF0">
    <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR10851">
    <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF10590">
    <property type="entry name" value="PNP_phzG_C"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF01243">
    <property type="entry name" value="PNPOx_N"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PIRSF" id="PIRSF000190">
    <property type="entry name" value="Pyd_amn-ph_oxd"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF50475">
    <property type="entry name" value="FMN-binding split barrel"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS01064">
    <property type="entry name" value="PYRIDOX_OXIDASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0285">Flavoprotein</keyword>
  <keyword id="KW-0288">FMN</keyword>
  <keyword id="KW-0560">Oxidoreductase</keyword>
  <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
  <keyword id="KW-1185">Reference proteome</keyword>
  <feature type="chain" id="PRO_0000167719" description="Pyridoxine/pyridoxamine 5'-phosphate oxidase">
    <location>
      <begin position="1"/>
      <end position="211"/>
    </location>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="8"/>
      <end position="11"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="61"/>
      <end position="66"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="66"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="76"/>
      <end position="77"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="83"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="105"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="123"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="127"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="131"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="140"/>
      <end position="141"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="184"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="190"/>
      <end position="192"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="194"/>
    </location>
    <ligand>
      <name>FMN</name>
      <dbReference type="ChEBI" id="CHEBI:58210"/>
    </ligand>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_01629"/>
    </source>
  </evidence>
  <sequence length="211" mass="24163" checksum="21482DDACDE734D6" modified="2004-10-25" version="1">MIDLHNIRNEYSQQALSEKQCDDDPLKQLEKWLNEAIQAKVNEPTAMNVATVGENGKPSSRVVLLKEVNERGLVFFTNYHSHKGRDLAVNPFAAVNLFWAELQRQVRVEGRVERISPQASDEYFASRPYTSRIGAWASEQSAVISGKNSLLTKAALIAAKHPLQVPRPPHWGGYIVIPELIEFWQGRPSRLHDRIRYRLEKGEWVRERLSP</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>