ID   Q5RC96_PONAB            Unreviewed;       640 AA.
AC   Q5RC96;
DT   21-DEC-2004, integrated into UniProtKB/TrEMBL.
DT   21-DEC-2004, sequence version 1.
DT   10-JUN-2026, entry version 78.
DE   RecName: Full=Exostosin-like 1 {ECO:0000256|ARBA:ARBA00074809};
DE            EC=2.4.1.224 {ECO:0000256|ARBA:ARBA00012194};
DE   AltName: Full=Exostosin-L {ECO:0000256|ARBA:ARBA00080873};
DE   AltName: Full=Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase {ECO:0000256|ARBA:ARBA00083239};
DE   AltName: Full=Multiple exostosis-like protein {ECO:0000256|ARBA:ARBA00082373};
GN   Name=DKFZp459K2032 {ECO:0000313|EMBL:CAH90611.1};
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601 {ECO:0000313|EMBL:CAH90611.1};
RN   [1] {ECO:0000313|EMBL:CAH90611.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Cortex {ECO:0000313|EMBL:CAH90611.1};
RG   The German cDNA Consortium;
RA   Koehrer K., Beyer A., Mewes H.W., Weil B., Amid C., Osanger A., Fobo G.,
RA   Han M., Wiemann S.;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Glycosyltransferase required for the biosynthesis of heparan-
CC       sulfate (HS). Transfers N-acetyl-alpha-D-glucosamine to the nascent HS
CC       chain (GlcNAcT-II activity). Appears to lack GlcNAcT I and GlcAT-II
CC       activities. {ECO:0000256|ARBA:ARBA00057834}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-O-{[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-
CC         beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-
CC         seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{alpha-D-
CC         GlcNAc-[(1->4)-beta-D-GlcA-(1->4)-alpha-D-GlcNAc](n)-(1->4)-beta-D-
CC         GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl}-L-seryl-
CC         [protein] + UDP + H(+); Xref=Rhea:RHEA:16213, Rhea:RHEA-COMP:12621,
CC         Rhea:RHEA-COMP:12623, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:132415, ChEBI:CHEBI:132416;
CC         EC=2.4.1.224; Evidence={ECO:0000256|ARBA:ARBA00052657};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|ARBA:ARBA00004922}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000256|ARBA:ARBA00004648}; Single-pass type II membrane protein
CC       {ECO:0000256|ARBA:ARBA00004648}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 47 family.
CC       {ECO:0000256|ARBA:ARBA00010271}.
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DR   EMBL; CR858383; CAH90611.1; -; mRNA.
DR   AlphaFoldDB; Q5RC96; -.
DR   KEGG; pon:100172231; -.
DR   OrthoDB; 5954868at2759; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050508; F:glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IEA:UniProtKB-ARBA.
DR   FunFam; 3.90.550.10:FF:000109; Exostosin like glycosyltransferase 1; 1.
DR   Gene3D; 3.90.550.10; Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A; 1.
DR   InterPro; IPR004263; Exostosin.
DR   InterPro; IPR040911; Exostosin_GT47.
DR   InterPro; IPR015338; GT64_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR48261; ACETYLGLUCOSAMINYLTRANSFERASE; 1.
DR   PANTHER; PTHR48261:SF3; EXOSTOSIN GLYCOSYLTRANSFERASE 1; 1.
DR   Pfam; PF03016; Exostosin_GT47; 1.
DR   Pfam; PF09258; Glyco_transf_64; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW   Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Signal-anchor {ECO:0000256|ARBA:ARBA00022968};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT   DOMAIN          96..292
FT                   /note="Exostosin GT47"
FT                   /evidence="ECO:0000259|Pfam:PF03016"
FT   DOMAIN          383..623
FT                   /note="Glycosyl transferase 64"
FT                   /evidence="ECO:0000259|Pfam:PF09258"
FT   REGION          574..594
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   640 AA;  70698 MW;  95E807AF96B5AB31 CRC64;
     MQSWRRRKSL WLALSASWLL LVLLGGFSLL RLALPPRPRP GASQGWPRWL DAELLQSFSQ
     PRELPEDTAS PPQAPHGGSC NWEACFDTSK CRGDGLKVFV YPAVGPISET HRRILASIEG
     SRFHTFSPAG ACLLLLLSLD AQTGECSSMP LQWNRGRNHL VLRLYPAACP RTFQLGQAMV
     AEASPTVDSF RPGFDVALPF LPEAHPLRGG APGQLRQHSP QPGVALLALE EERGGWRTAD
     TGSSACPWDG RCEQDPGPGQ TQHQETLPNA TFCLISGHRP EAASRFLQAL QVLAALQEMS
     PARVLALRQQ TQFLWDAYFS SVEKVIHTTL EVIQDRIFGT SAHPSLLWNS PPGALLALST
     FSTSPQDFPF YHLQQGSRPE GRFSALIWVA PPGQPPLKLI QAVAGSQHCA QILVLWSNER
     PLPSRWPETA VPLTVIDGHR KVSDRFYPYS TIRTDAILSL DARSSLSTSE VDFAFLVWQS
     FPERMVGFLT LSHFWDEAHG GWGYTAERTN EFSMVLTTAA FYHRYYHTLF THSLPKALRT
     LADEAPTCVD VLMNFIVAAV TKLPPIKVPY GKQRQEAAPL APGGPGPRSK PQAPAPDCIN
     QIAAAFGHMP LLSSCLRLDP VLFKDPVSVQ RKKYRSLEKP
//