ID Q5LP92_RUEPO Unreviewed; 427 AA. AC Q5LP92; DT 01-FEB-2005, integrated into UniProtKB/TrEMBL. DT 01-FEB-2005, sequence version 1. DT 04-NOV-2026, entry version 106. DE RecName: Full=Guanine deaminase {ECO:0000256|ARBA:ARBA00012781, ECO:0000256|NCBIfam:TIGR02967}; DE Short=Guanase {ECO:0000256|RuleBase:RU366009}; DE EC=3.5.4.3 {ECO:0000256|ARBA:ARBA00012781, ECO:0000256|NCBIfam:TIGR02967}; DE AltName: Full=Guanine aminohydrolase {ECO:0000256|RuleBase:RU366009}; GN OrderedLocusNames=SPO2956 {ECO:0000313|EMBL:AAV96197.1}; OS Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter OS pomeroyi). OC Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria; OC Rhodobacterales; Roseobacteraceae; Ruegeria. OX NCBI_TaxID=246200 {ECO:0000313|EMBL:AAV96197.1, ECO:0000313|Proteomes:UP000001023}; RN [1] {ECO:0000313|EMBL:AAV96197.1, ECO:0000313|Proteomes:UP000001023} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3 RC {ECO:0000313|Proteomes:UP000001023}; RX PubMed=15602564; DOI=10.1038/nature03170; RA Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B., RA Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L., RA Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M., RA Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q., RA Daugherty S.C., Deboy R.T., Dodson R.J., Durkin A.S., Madupu R., RA Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N.; RT "Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine RT environment."; RL Nature 432:910-913(2004). RN [2] {ECO:0000313|EMBL:AAV96197.1, ECO:0000313|Proteomes:UP000001023} RP GENOME REANNOTATION. RC STRAIN=ATCC 700808 / DSM 15171 / DSS-3 RC {ECO:0000313|Proteomes:UP000001023}; RX PubMed=25780504; DOI=10.1186/1944-3277-9-11; RA Rivers A.R., Smith C.B., Moran M.A.; RT "An updated genome annotation for the model marine bacterium Ruegeria RT pomeroyi DSS-3."; RL Stand. Genomic Sci. 9:11-11(2014). CC -!- FUNCTION: Catalyzes the hydrolytic deamination of guanine, producing CC xanthine and ammonia. {ECO:0000256|RuleBase:RU366009}. CC -!- CATALYTIC ACTIVITY: CC Reaction=guanine + H2O + H(+) = xanthine + NH4(+); CC Xref=Rhea:RHEA:14665, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:16235, ChEBI:CHEBI:17712, ChEBI:CHEBI:28938; EC=3.5.4.3; CC Evidence={ECO:0000256|RuleBase:RU366009}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|RuleBase:RU366009}; CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|RuleBase:RU366009}; CC -!- PATHWAY: Purine metabolism; guanine degradation; xanthine from guanine: CC step 1/1. {ECO:0000256|ARBA:ARBA00004984, CC ECO:0000256|RuleBase:RU366009}. CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily. CC ATZ/TRZ family. {ECO:0000256|ARBA:ARBA00006745, CC ECO:0000256|RuleBase:RU366009}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000031; AAV96197.1; -; Genomic_DNA. DR RefSeq; WP_011048655.1; NC_003911.12. DR AlphaFoldDB; Q5LP92; -. DR STRING; 246200.SPO2956; -. DR PaxDb; 246200-SPO2956; -. DR KEGG; sil:SPO2956; -. DR eggNOG; COG0402; Bacteria. DR HOGENOM; CLU_012358_0_2_5; -. DR OMA; AADFWEA; -. DR OrthoDB; 9787621at2; -. DR UniPathway; UPA00603; UER00660. DR Proteomes; UP000001023; Chromosome. DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter. DR GO; GO:0008892; F:guanine deaminase activity; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006147; P:guanine catabolic process; IEA:UniProtKB-UniRule. DR CDD; cd01303; GDEase; 1. DR FunFam; 3.20.20.140:FF:000022; Guanine deaminase; 1. DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1. DR Gene3D; 2.30.40.10; Urease, subunit C, domain 1; 1. DR InterPro; IPR006680; Amidohydro-rel. DR InterPro; IPR014311; Guanine_deaminase. DR InterPro; IPR011059; Metal-dep_hydrolase_composite. DR InterPro; IPR032466; Metal_Hydrolase. DR InterPro; IPR051607; Metallo-dep_hydrolases. DR InterPro; IPR054418; MQNX/HUTI_composite_N. DR NCBIfam; TIGR02967; guan_deamin; 1. DR NCBIfam; NF006679; PRK09228.1; 1. DR PANTHER; PTHR11271; GUANINE DEAMINASE; 1. DR PANTHER; PTHR11271:SF6; GUANINE DEAMINASE; 1. DR Pfam; PF01979; Amidohydro_1; 1. DR Pfam; PF22039; HUTI_composite_bact; 1. DR SUPFAM; SSF51338; Composite domain of metallo-dependent hydrolases; 1. DR SUPFAM; SSF51556; Metallo-dependent hydrolases; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366009}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|RuleBase:RU366009}; KW Reference proteome {ECO:0000313|Proteomes:UP000001023}; KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU366009}. FT DOMAIN 32..56 FT /note="Aminodeoxyfutalosine FT deaminase/Imidazolonepropionase-like composite" FT /evidence="ECO:0000259|Pfam:PF22039" FT DOMAIN 65..427 FT /note="Amidohydrolase-related" FT /evidence="ECO:0000259|Pfam:PF01979" SQ SEQUENCE 427 AA; 46937 MW; EDE61E1EC5E6F089 CRC64; MTGQTILRGR VLSFVQSPFD TAPETAVREN EALLIEGGHV TAVGSWDALR REAPQAEVSD HGERVILAGF VDAHVHYPQT AMIASWGKRL IDWLNTYTFP EERKFADLTY ASEIAARYLD LTAAHGTTTM CSFCTIHPES VEAFFAEAQA RGQRVVAGKT CMDRNAPDFL RDTAQSAYDQ SKALLQKWHG VDRISYAITP RFSPTSTSEQ LDAMGALWAE HPDCLMQTHL SEQTDEIEWV KGLYPEARDY LDTYEQHGLL GGCGLYGHAI HLEKRERDRL REHGAGLIHC PTSNTFIGSG LFDMAGLMAE GQRIGLATDT GGGSSFSMLR TMAAAYEIGQ LRGVPLHAAQ LLWLATQGSA RALRMDHQIG NIAPGMEADL VVLDLASTPA IAQRSAMADD LWEALFPTIM MGDDRAIAEV WIAGRRV //