ID   Q51110_NEIME            Unreviewed;       329 AA.
AC   Q51110;
DT   01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1996, sequence version 1.
DT   02-SEP-2026, entry version 61.
DE   RecName: Full=lipopolysaccharide heptosyltransferase II {ECO:0000256|ARBA:ARBA00044042};
DE            EC=2.4.99.24 {ECO:0000256|ARBA:ARBA00044042};
GN   Name=lsi1 {ECO:0000313|EMBL:AAC44079.1};
OS   Neisseria meningitidis.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria; Neisseriales;
OC   Neisseriaceae; Neisseria.
OX   NCBI_TaxID=487 {ECO:0000313|EMBL:AAC44079.1};
RN   [1] {ECO:0000313|EMBL:AAC44079.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=MC58 {ECO:0000313|EMBL:AAC44079.1};
RX   PubMed=8852280; DOI=10.1006/mpat.1995.0074;
RA   Jennings M.P., Bisercic M., Dunn K.L.R., Martin A., Wilkes K.E., Virji M.,
RA   Richards J.C., Moxon E.R.;
RT   "Cloning and molecular analysis of the Isi1 (rfaF) gene of Neisseria
RT   meningitidis which encodes a heptosyl-2-transferase involved in LPS
RT   biosynthesis: evaluation of surface exposed carbohydrates in LPS mediated
RT   toxicity for human endothelial cells.";
RL   Microb. Pathog. 19:391-407(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an L-alpha-D-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-
CC         lipid A + ADP-L-glycero-beta-D-manno-heptose = an L-alpha-D-Hep-
CC         (1->3)-L-alpha-D-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-lipid
CC         A + ADP + H(+); Xref=Rhea:RHEA:74071, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:61506, ChEBI:CHEBI:193068, ChEBI:CHEBI:193069,
CC         ChEBI:CHEBI:456216; EC=2.4.99.24;
CC         Evidence={ECO:0000256|ARBA:ARBA00047503};
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 9 family.
CC       {ECO:0000256|ARBA:ARBA00043995}.
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DR   EMBL; U23782; AAC44079.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q51110; -.
DR   CAZy; GT9; Glycosyltransferase Family 9.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0008713; F:ADP-heptose-lipopolysaccharide heptosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR   FunFam; 3.40.50.2000:FF:000023; ADP-heptose--LPS heptosyltransferase II; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR   InterPro; IPR002201; Glyco_trans_9.
DR   InterPro; IPR011910; RfaF.
DR   NCBIfam; TIGR02195; heptsyl_trn_II; 1.
DR   PANTHER; PTHR30160:SF7; ADP-HEPTOSE--LPS HEPTOSYLTRANSFERASE 2; 1.
DR   PANTHER; PTHR30160; TETRAACYLDISACCHARIDE 4'-KINASE-RELATED; 1.
DR   Pfam; PF01075; Glyco_transf_9; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AAC44079.1}.
SQ   SEQUENCE   329 AA;  37095 MW;  244D37078AC8E51C CRC64;
     MTQPLFRRLK ELHPGCTIDV FAPKWSMAVF ERRPEVNEIL ENSFGHGALE LKRRWRVGRE
     LGRRGYDQVI VLPGSLKSAI IALATGSVKG RVMSVKARYF LLNDIRRLDK ERLPLMVDRY
     TALAHPSQED FDGHSGFPEF SIDERRREIS VETFGLDIGK PVLAFCPGAE FGPAKRWPTR
     HFAELGKHYL AAGWQVWLFG SQKDDEIAEE INRLSDGMCV NLCGKTDLSQ AMDLLSLADT
     VVCNDSGLMH LAAALGRKVV AVYGSSSPTH TPPLSDRAKI VSLHLECSPC FKRECRWGIP
     TASTGCIPRR LCRRLKRRYD GFCFRVYAV
//