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<entry dataset="Swiss-Prot" created="2005-11-22" modified="2026-09-02" version="84" xmlns="http://uniprot.org/uniprot">
  <accession>Q4R8T9</accession>
  <accession>Q4R954</accession>
  <name>CDKL3_MACFA</name>
  <protein>
    <recommendedName>
      <fullName evidence="2">Cyclin-dependent kinase-like 3</fullName>
      <ecNumber>2.7.11.22</ecNumber>
    </recommendedName>
  </protein>
  <gene>
    <name evidence="2" type="primary">CDKL3</name>
    <name type="ORF">QtsA-10691</name>
    <name type="ORF">QtsA-11477</name>
  </gene>
  <organism>
    <name type="scientific">Macaca fascicularis</name>
    <name type="common">Crab-eating macaque</name>
    <name type="synonym">Cynomolgus monkey</name>
    <dbReference type="NCBI Taxonomy" id="9541"/>
    <lineage>
      <taxon>Eukaryota</taxon>
      <taxon>Metazoa</taxon>
      <taxon>Chordata</taxon>
      <taxon>Craniata</taxon>
      <taxon>Vertebrata</taxon>
      <taxon>Euteleostomi</taxon>
      <taxon>Mammalia</taxon>
      <taxon>Eutheria</taxon>
      <taxon>Euarchontoglires</taxon>
      <taxon>Primates</taxon>
      <taxon>Haplorrhini</taxon>
      <taxon>Catarrhini</taxon>
      <taxon>Cercopithecidae</taxon>
      <taxon>Cercopithecinae</taxon>
      <taxon>Macaca</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="submission" date="2005-06" db="EMBL/GenBank/DDBJ databases">
      <title>DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications.</title>
      <authorList>
        <consortium name="International consortium for macaque cDNA sequencing and analysis"/>
      </authorList>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]</scope>
    <source>
      <tissue>Testis</tissue>
    </source>
  </reference>
  <comment type="catalytic activity">
    <reaction>
      <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
      <dbReference type="Rhea" id="RHEA:17989"/>
      <dbReference type="Rhea" id="RHEA-COMP:9863"/>
      <dbReference type="Rhea" id="RHEA-COMP:11604"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:29999"/>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
      <dbReference type="ChEBI" id="CHEBI:83421"/>
      <dbReference type="ChEBI" id="CHEBI:456216"/>
      <dbReference type="EC" id="2.7.11.22"/>
    </reaction>
  </comment>
  <comment type="catalytic activity">
    <reaction>
      <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
      <dbReference type="Rhea" id="RHEA:46608"/>
      <dbReference type="Rhea" id="RHEA-COMP:11060"/>
      <dbReference type="Rhea" id="RHEA-COMP:11605"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:30013"/>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
      <dbReference type="ChEBI" id="CHEBI:61977"/>
      <dbReference type="ChEBI" id="CHEBI:456216"/>
      <dbReference type="EC" id="2.7.11.22"/>
    </reaction>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="domain">
    <text>The [NKR]KIAxRE motif seems to be a cyclin-binding region.</text>
  </comment>
  <comment type="similarity">
    <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
  </comment>
  <dbReference type="EC" id="2.7.11.22"/>
  <dbReference type="EMBL" id="AB168359">
    <property type="protein sequence ID" value="BAE00482.1"/>
    <property type="molecule type" value="mRNA"/>
  </dbReference>
  <dbReference type="EMBL" id="AB168242">
    <property type="protein sequence ID" value="BAE00367.1"/>
    <property type="molecule type" value="mRNA"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q4R8T9"/>
  <dbReference type="SMR" id="Q4R8T9"/>
  <dbReference type="STRING" id="9541.ENSMFAP00000015535"/>
  <dbReference type="eggNOG" id="KOG0593">
    <property type="taxonomic scope" value="Eukaryota"/>
  </dbReference>
  <dbReference type="Proteomes" id="UP000233100">
    <property type="component" value="Unplaced"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005737">
    <property type="term" value="C:cytoplasm"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-SubCell"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005634">
    <property type="term" value="C:nucleus"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005524">
    <property type="term" value="F:ATP binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0004693">
    <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-EC"/>
  </dbReference>
  <dbReference type="GO" id="GO:0106310">
    <property type="term" value="F:protein serine kinase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="RHEA"/>
  </dbReference>
  <dbReference type="GO" id="GO:0097484">
    <property type="term" value="P:dendrite extension"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0030517">
    <property type="term" value="P:negative regulation of axon extension"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0050775">
    <property type="term" value="P:positive regulation of dendrite morphogenesis"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="CDD" id="cd07846">
    <property type="entry name" value="STKc_CDKL2_3"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.30.200.20:FF:000049">
    <property type="entry name" value="cyclin-dependent kinase-like 1 isoform X1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="1.10.510.10:FF:000370">
    <property type="entry name" value="cyclin-dependent kinase-like 3 isoform X2"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.30.200.20">
    <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="1.10.510.10">
    <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR050108">
    <property type="entry name" value="CDK"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011009">
    <property type="entry name" value="Kinase-like_dom_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR000719">
    <property type="entry name" value="Prot_kinase_dom"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR017441">
    <property type="entry name" value="Protein_kinase_ATP_BS"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR008271">
    <property type="entry name" value="Ser/Thr_kinase_AS"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR24056">
    <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR24056:SF177">
    <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 3"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00069">
    <property type="entry name" value="Pkinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SMART" id="SM00220">
    <property type="entry name" value="S_TKc"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF56112">
    <property type="entry name" value="Protein kinase-like (PK-like)"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS00107">
    <property type="entry name" value="PROTEIN_KINASE_ATP"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS50011">
    <property type="entry name" value="PROTEIN_KINASE_DOM"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS00108">
    <property type="entry name" value="PROTEIN_KINASE_ST"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="evidence at transcript level"/>
  <keyword id="KW-0067">ATP-binding</keyword>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-0418">Kinase</keyword>
  <keyword id="KW-0547">Nucleotide-binding</keyword>
  <keyword id="KW-0597">Phosphoprotein</keyword>
  <keyword id="KW-1185">Reference proteome</keyword>
  <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
  <keyword id="KW-0808">Transferase</keyword>
  <feature type="chain" id="PRO_0000085821" description="Cyclin-dependent kinase-like 3">
    <location>
      <begin position="1"/>
      <end position="590"/>
    </location>
  </feature>
  <feature type="domain" description="Protein kinase" evidence="4">
    <location>
      <begin position="4"/>
      <end position="286"/>
    </location>
  </feature>
  <feature type="region of interest" description="Disordered" evidence="6">
    <location>
      <begin position="459"/>
      <end position="508"/>
    </location>
  </feature>
  <feature type="region of interest" description="Disordered" evidence="6">
    <location>
      <begin position="547"/>
      <end position="590"/>
    </location>
  </feature>
  <feature type="short sequence motif" description="[NKR]KIAxRE">
    <location>
      <begin position="45"/>
      <end position="51"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Polar residues" evidence="6">
    <location>
      <begin position="466"/>
      <end position="477"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
    <location>
      <begin position="547"/>
      <end position="556"/>
    </location>
  </feature>
  <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
    <location>
      <begin position="580"/>
      <end position="590"/>
    </location>
  </feature>
  <feature type="active site" description="Proton acceptor" evidence="4 5">
    <location>
      <position position="125"/>
    </location>
  </feature>
  <feature type="binding site" evidence="4">
    <location>
      <begin position="10"/>
      <end position="18"/>
    </location>
    <ligand>
      <name>ATP</name>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="4">
    <location>
      <position position="33"/>
    </location>
    <ligand>
      <name>ATP</name>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
    </ligand>
  </feature>
  <feature type="modified residue" description="Phosphothreonine" evidence="3">
    <location>
      <position position="158"/>
    </location>
  </feature>
  <feature type="modified residue" description="Phosphotyrosine" evidence="3">
    <location>
      <position position="160"/>
    </location>
  </feature>
  <feature type="sequence conflict" description="In Ref. 1; BAE00367." evidence="7" ref="1">
    <location>
      <begin position="117"/>
      <end position="201"/>
    </location>
  </feature>
  <feature type="sequence conflict" description="In Ref. 1; BAE00367." evidence="7" ref="1">
    <original>G</original>
    <variation>E</variation>
    <location>
      <position position="490"/>
    </location>
  </feature>
  <feature type="sequence conflict" description="In Ref. 1; BAE00367." evidence="7" ref="1">
    <original>N</original>
    <variation>S</variation>
    <location>
      <position position="495"/>
    </location>
  </feature>
  <evidence type="ECO:0000250" key="1"/>
  <evidence type="ECO:0000250" key="2">
    <source>
      <dbReference type="UniProtKB" id="Q8IVW4"/>
    </source>
  </evidence>
  <evidence type="ECO:0000250" key="3">
    <source>
      <dbReference type="UniProtKB" id="Q9JM01"/>
    </source>
  </evidence>
  <evidence type="ECO:0000255" key="4">
    <source>
      <dbReference type="PROSITE-ProRule" id="PRU00159"/>
    </source>
  </evidence>
  <evidence type="ECO:0000255" key="5">
    <source>
      <dbReference type="PROSITE-ProRule" id="PRU10027"/>
    </source>
  </evidence>
  <evidence type="ECO:0000256" key="6">
    <source>
      <dbReference type="SAM" id="MobiDB-lite"/>
    </source>
  </evidence>
  <evidence type="ECO:0000305" key="7"/>
  <sequence length="590" mass="67204" checksum="C47879E193EE3658" modified="2005-07-19" version="1">MEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSDLLHHEYFTRDGFIEKFMPELKAKLLQEAKVNSLIKPKESSKENELRKDERKTVYTNTLLSSSVLGKEIEKEKKPKEIKVRVIKVKGGRGDISEPKKKEYEGGLCQQDANENVHPMSPDTKLVTIEPPNPINPSTNCNGLKENPHCGGSMTMPPINLTNSNLMAANLNSNLFHPSVRLTERAKKRRTSSQSIGQVMPNSRQEDPGPIQSQMGKGIFNERTGHSDQMSNENKRKLNFSRSDRKEFHFPELPVTIQPKDTKGMEVKQIKMLKRESKKTDSSKIPTLLNVDQNQEKQENTGNAQTERKKNLPDVE</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>