ID   EXPA9_ORYSJ             Reviewed;         254 AA.
AC   Q4PR53; Q9XHW9;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 2.
DT   10-JUN-2026, entry version 107.
DE   RecName: Full=Expansin-A9;
DE   AltName: Full=Alpha-expansin-9;
DE   AltName: Full=OsEXP9;
DE   AltName: Full=OsEXPA9;
DE   AltName: Full=OsaEXPa1.19;
DE   Flags: Precursor;
GN   Name=EXPA9; Synonyms=EXP9;
GN   OrderedLocusNames=Os01g0249100, LOC_Os01g14660;
GN   ORFNames=OSJNBa0049B20.24, P0034C11.32;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 5-222, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Dongjin;
RX   PubMed=16267395; DOI=10.1016/s1016-8478(23)13219-5;
RA   Shin J.-H., Jeong D.-H., Park M.C., An G.;
RT   "Characterization and transcriptional expression of the alpha-expansin gene
RT   family in rice.";
RL   Mol. Cells 20:210-218(2005).
RN   [6]
RP   NOMENCLATURE.
RX   PubMed=15604683; DOI=10.1007/s11103-004-0158-6;
RA   Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J.,
RA   Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C.,
RA   Voesenek L.A.C.;
RT   "Nomenclature for members of the expansin superfamily of genes and
RT   proteins.";
RL   Plant Mol. Biol. 55:311-314(2004).
CC   -!- FUNCTION: May cause loosening and extension of plant cell walls by
CC       disrupting non-covalent bonding between cellulose microfibrils and
CC       matrix glucans. No enzymatic activity has been found. May be required
CC       for rapid internodal elongation in deepwater rice during submergence
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:16267395}.
CC   -!- SIMILARITY: Belongs to the expansin family. Expansin A subfamily.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=EXPANSIN homepage;
CC       URL="https://www.dept.psu.edu/biology/groups/expansins/index.htm";
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DR   EMBL; AC007789; AAD38297.1; -; Genomic_DNA.
DR   EMBL; AP002865; BAB18338.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF04502.1; -; Genomic_DNA.
DR   EMBL; AP014957; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ061054; AAY63545.1; -; mRNA.
DR   AlphaFoldDB; Q4PR53; -.
DR   SMR; Q4PR53; -.
DR   FunCoup; Q4PR53; 8.
DR   STRING; 39947.Q4PR53; -.
DR   GlyCosmos; Q4PR53; 4 sites, No reported glycans.
DR   PaxDb; 39947-Q4PR53; -.
DR   KEGG; dosa:Os01g0249100; -.
DR   HOGENOM; CLU_027462_0_0_1; -.
DR   InParanoid; Q4PR53; -.
DR   OrthoDB; 586465at2759; -.
DR   Proteomes; UP000059680; Chromosome 1.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009828; P:plant-type cell wall loosening; ISS:UniProtKB.
DR   CDD; cd22274; DPBB_EXPA_N; 1.
DR   FunFam; 2.60.40.760:FF:000001; Expansin; 1.
DR   Gene3D; 2.60.40.760; Expansin, cellulose-binding-like domain; 1.
DR   Gene3D; 2.40.40.10; RlpA-like domain; 1.
DR   InterPro; IPR007118; Expan_Lol_pI.
DR   InterPro; IPR002963; Expansin.
DR   InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR   InterPro; IPR007117; Expansin_CBD.
DR   InterPro; IPR036749; Expansin_CBD_sf.
DR   InterPro; IPR009009; RlpA-like_DPBB.
DR   InterPro; IPR036908; RlpA-like_sf.
DR   PANTHER; PTHR31867; EXPANSIN-A15; 1.
DR   Pfam; PF03330; DPBB_1; 1.
DR   Pfam; PF01357; Expansin_C; 1.
DR   PRINTS; PR01226; EXPANSIN.
DR   PRINTS; PR01225; EXPANSNFAMLY.
DR   SMART; SM00837; DPBB_1; 1.
DR   SUPFAM; SSF50685; Barwin-like endoglucanases; 1.
DR   SUPFAM; SSF49590; PHL pollen allergen; 1.
DR   PROSITE; PS50843; EXPANSIN_CBD; 1.
DR   PROSITE; PS50842; EXPANSIN_EG45; 1.
PE   2: Evidence at transcript level;
KW   Cell wall; Cell wall biogenesis/degradation; Disulfide bond; Glycoprotein;
KW   Membrane; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..254
FT                   /note="Expansin-A9"
FT                   /id="PRO_0000251988"
FT   DOMAIN          45..159
FT                   /note="Expansin-like EG45"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DOMAIN          169..248
FT                   /note="Expansin-like CBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT   CARBOHYD        61
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        94
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        109
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        251
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        48..76
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DISULFID        79..154
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT   DISULFID        84..92
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
SQ   SEQUENCE   254 AA;  26987 MW;  A40924E21A819101 CRC64;
     MEKKLLVVLF LSLCCASRLR GEAAQQWTSA TATFYGGSDA SGTMGGSCGY GNMYSAGYGT
     NTTALSSALY GDGASCGACY LVTCDASATR WCKNGTSVTV TATNYCPPNY SESGDAGGWC
     NPPRRHFDMS QPAWEAIAVY SSGIVPVRYA RTPCRRVGGI RFGIAGHDYY ELVLVTNVAG
     SGAVAAAWVK GSGTEWLSMS RNWGENWQSN AYLTGQALSF RVQADDGGVV TAYDVAPANW
     QFGSTYQSDV NFSY
//