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<entry dataset="Swiss-Prot" created="2006-04-04" modified="2026-11-04" version="114" xmlns="http://uniprot.org/uniprot">
  <accession>Q4K5T9</accession>
  <name>PROB_PSEF5</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">Glutamate 5-kinase</fullName>
      <ecNumber evidence="1">2.7.2.11</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">Gamma-glutamyl kinase</fullName>
      <shortName evidence="1">GK</shortName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">proB</name>
    <name type="ordered locus">PFL_5326</name>
  </gene>
  <organism>
    <name type="scientific">Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)</name>
    <dbReference type="NCBI Taxonomy" id="220664"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Pseudomonadales</taxon>
      <taxon>Pseudomonadaceae</taxon>
      <taxon>Pseudomonas</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2005" name="Nat. Biotechnol." volume="23" first="873" last="878">
      <title>Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.</title>
      <authorList>
        <person name="Paulsen I.T."/>
        <person name="Press C.M."/>
        <person name="Ravel J."/>
        <person name="Kobayashi D.Y."/>
        <person name="Myers G.S.A."/>
        <person name="Mavrodi D.V."/>
        <person name="DeBoy R.T."/>
        <person name="Seshadri R."/>
        <person name="Ren Q."/>
        <person name="Madupu R."/>
        <person name="Dodson R.J."/>
        <person name="Durkin A.S."/>
        <person name="Brinkac L.M."/>
        <person name="Daugherty S.C."/>
        <person name="Sullivan S.A."/>
        <person name="Rosovitz M.J."/>
        <person name="Gwinn M.L."/>
        <person name="Zhou L."/>
        <person name="Schneider D.J."/>
        <person name="Cartinhour S.W."/>
        <person name="Nelson W.C."/>
        <person name="Weidman J."/>
        <person name="Watkins K."/>
        <person name="Tran K."/>
        <person name="Khouri H."/>
        <person name="Pierson E.A."/>
        <person name="Pierson L.S. III"/>
        <person name="Thomashow L.S."/>
        <person name="Loper J.E."/>
      </authorList>
      <dbReference type="PubMed" id="15980861"/>
      <dbReference type="DOI" id="10.1038/nbt1110"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>ATCC BAA-477 / NRRL B-23932 / Pf-5</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>L-glutamate + ATP = L-glutamyl 5-phosphate + ADP</text>
      <dbReference type="Rhea" id="RHEA:14877"/>
      <dbReference type="ChEBI" id="CHEBI:29985"/>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
      <dbReference type="ChEBI" id="CHEBI:58274"/>
      <dbReference type="ChEBI" id="CHEBI:456216"/>
      <dbReference type="EC" id="2.7.2.11"/>
    </reaction>
  </comment>
  <comment type="pathway">
    <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the glutamate 5-kinase family.</text>
  </comment>
  <dbReference type="EC" id="2.7.2.11" evidence="1"/>
  <dbReference type="EMBL" id="CP000076">
    <property type="protein sequence ID" value="AAY94536.2"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_011063553.1">
    <property type="nucleotide sequence ID" value="NC_004129.6"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q4K5T9"/>
  <dbReference type="SMR" id="Q4K5T9"/>
  <dbReference type="STRING" id="220664.PFL_5326"/>
  <dbReference type="GeneID" id="57478295"/>
  <dbReference type="KEGG" id="pfl:PFL_5326"/>
  <dbReference type="PATRIC" id="fig|220664.5.peg.5436"/>
  <dbReference type="eggNOG" id="COG0263">
    <property type="taxonomic scope" value="Bacteria"/>
  </dbReference>
  <dbReference type="HOGENOM" id="CLU_025400_2_0_6"/>
  <dbReference type="OMA" id="AACGQSH"/>
  <dbReference type="UniPathway" id="UPA00098">
    <property type="reaction ID" value="UER00359"/>
  </dbReference>
  <dbReference type="Proteomes" id="UP000008540">
    <property type="component" value="Chromosome"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005829">
    <property type="term" value="C:cytosol"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005524">
    <property type="term" value="F:ATP binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0004349">
    <property type="term" value="F:glutamate 5-kinase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0003723">
    <property type="term" value="F:RNA binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0055129">
    <property type="term" value="P:L-proline biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="CDD" id="cd04242">
    <property type="entry name" value="AAK_G5K_ProB"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="CDD" id="cd21157">
    <property type="entry name" value="PUA_G5K"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="2.30.130.10:FF:000007">
    <property type="entry name" value="Glutamate 5-kinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.40.1160.10:FF:000018">
    <property type="entry name" value="Glutamate 5-kinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.40.1160.10">
    <property type="entry name" value="Acetylglutamate kinase-like"/>
    <property type="match status" value="2"/>
  </dbReference>
  <dbReference type="Gene3D" id="2.30.130.10">
    <property type="entry name" value="PUA domain"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_00456">
    <property type="entry name" value="ProB"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR036393">
    <property type="entry name" value="AceGlu_kinase-like_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR001048">
    <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR041739">
    <property type="entry name" value="G5K_ProB"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR001057">
    <property type="entry name" value="Glu/AcGlu_kinase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR011529">
    <property type="entry name" value="Glu_5kinase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR005715">
    <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR019797">
    <property type="entry name" value="Glutamate_5-kinase_CS"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR002478">
    <property type="entry name" value="PUA"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR015947">
    <property type="entry name" value="PUA-like_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR036974">
    <property type="entry name" value="PUA_sf"/>
  </dbReference>
  <dbReference type="NCBIfam" id="TIGR01027">
    <property type="entry name" value="proB"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43654">
    <property type="entry name" value="GLUTAMATE 5-KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR43654:SF1">
    <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00696">
    <property type="entry name" value="AA_kinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF01472">
    <property type="entry name" value="PUA"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PIRSF" id="PIRSF000729">
    <property type="entry name" value="GK"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PRINTS" id="PR00474">
    <property type="entry name" value="GLU5KINASE"/>
  </dbReference>
  <dbReference type="SMART" id="SM00359">
    <property type="entry name" value="PUA"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF53633">
    <property type="entry name" value="Carbamate kinase-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF88697">
    <property type="entry name" value="PUA domain-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS00902">
    <property type="entry name" value="GLUTAMATE_5_KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PROSITE" id="PS50890">
    <property type="entry name" value="PUA"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
  <keyword id="KW-0067">ATP-binding</keyword>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-0418">Kinase</keyword>
  <keyword id="KW-0547">Nucleotide-binding</keyword>
  <keyword id="KW-0641">Proline biosynthesis</keyword>
  <keyword id="KW-1185">Reference proteome</keyword>
  <keyword id="KW-0808">Transferase</keyword>
  <feature type="chain" id="PRO_0000230057" description="Glutamate 5-kinase">
    <location>
      <begin position="1"/>
      <end position="372"/>
    </location>
  </feature>
  <feature type="domain" description="PUA" evidence="1">
    <location>
      <begin position="280"/>
      <end position="358"/>
    </location>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="14"/>
    </location>
    <ligand>
      <name>ATP</name>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="54"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="141"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="153"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="173"/>
      <end position="174"/>
    </location>
    <ligand>
      <name>ATP</name>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
    </ligand>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_00456"/>
    </source>
  </evidence>
  <sequence length="372" mass="39600" checksum="C596F89D800EB617" modified="2012-03-21" version="2">MRSKVTGAQRWVVKIGSALLTADGKGLDRTAMGVWVEQMVALHEAGVELVLVSSGAVAAGMSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYLNARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFDADPRNNPDAQLIYEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVGGRLDRVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDEGAVSALSQGNKSLLPVGVKQVQGGFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQPSDAIVGVLGYMAEPELVHRDNLILV</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>