ID TTC31_HUMAN Reviewed; 519 AA. AC Q49AM3; Q4KN40; Q53FD4; Q9H9F7; DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot. DT 25-NOV-2008, sequence version 3. DT 10-JUN-2026, entry version 143. DE RecName: Full=Tetratricopeptide repeat protein 31; DE Short=TPR repeat protein 31; GN Name=TTC31; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., RA Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15815621; DOI=10.1038/nature03466; RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., RA Wilson R.K.; RT "Generation and annotation of the DNA sequences of human chromosomes 2 and RT 4."; RL Nature 434:724-731(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT RP VAL-28. RC TISSUE=Testis; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-369. RC TISSUE=Testis; RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., RA Tanaka A., Yokoyama S.; RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Cervix carcinoma, and Erythroleukemia; RX PubMed=23186163; DOI=10.1021/pr300630k; RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., RA Mohammed S.; RT "Toward a comprehensive characterization of a human cancer cell RT phosphoproteome."; RL J. Proteome Res. 12:260-271(2013). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q49AM3-1; Sequence=Displayed; CC Name=2; CC IsoId=Q49AM3-2; Sequence=VSP_023990, VSP_023991; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK022850; BAB14272.1; -; mRNA. DR EMBL; AC005041; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC036017; AAH36017.1; -; mRNA. DR EMBL; BC096709; AAH96709.1; -; mRNA. DR EMBL; AK223355; BAD97075.1; -; mRNA. DR CCDS; CCDS42701.1; -. [Q49AM3-1] DR RefSeq; NP_071937.4; NM_022492.6. [Q49AM3-1] DR AlphaFoldDB; Q49AM3; -. DR SMR; Q49AM3; -. DR BioGRID; 122175; 26. DR FunCoup; Q49AM3; 520. DR IntAct; Q49AM3; 8. DR NDEx; MUSIC2-C5197-TTC31; Osteosarcoma (U2OS) cell map - C5197 (43 proteins). DR STRING; 9606.ENSP00000233623; -. DR iPTMnet; Q49AM3; -. DR PhosphoSitePlus; Q49AM3; -. DR BioMuta; TTC31; -. DR DMDM; 215274203; -. DR jPOST; Q49AM3; -. DR MassIVE; Q49AM3; -. DR PaxDb; 9606-ENSP00000233623; -. DR PeptideAtlas; Q49AM3; -. DR ProteomicsDB; 62056; -. [Q49AM3-1] DR ProteomicsDB; 62057; -. [Q49AM3-2] DR Pumba; Q49AM3; -. DR Antibodypedia; 31535; 26 antibodies from 12 providers. DR DNASU; 64427; -. DR Ensembl; ENST00000233623.11; ENSP00000233623.6; ENSG00000115282.22. [Q49AM3-1] DR Ensembl; ENST00000442235.6; ENSP00000416823.3; ENSG00000115282.22. [Q49AM3-2] DR GeneID; 64427; -. DR KEGG; hsa:64427; -. DR MANE-Select; ENST00000233623.11; ENSP00000233623.6; NM_022492.6; NP_071937.4. DR UCSC; uc002slt.3; human. [Q49AM3-1] DR AGR; HGNC:25759; -. DR ClinPGx; PA145147843; -. DR CTD; 64427; -. DR DisGeNET; 64427; -. DR GeneCards; TTC31; -. DR HGNC; HGNC:25759; TTC31. DR HPA; ENSG00000115282; Low tissue specificity. DR OpenTargets; ENSG00000115282; -. DR VEuPathDB; HostDB:ENSG00000115282; -. DR eggNOG; KOG0548; Eukaryota. DR GeneTree; ENSGT00940000161036; -. DR HOGENOM; CLU_039740_1_0_1; -. DR InParanoid; Q49AM3; -. DR OMA; HFCDEGK; -. DR OrthoDB; 2423701at2759; -. DR PAN-GO; Q49AM3; 0 GO annotations based on evolutionary models. DR PhylomeDB; Q49AM3; -. DR PathwayCommons; Q49AM3; -. DR SignaLink; Q49AM3; -. DR Agora; ENSG00000115282; -. DR BioGRID-ORCS; 64427; 8 hits in 1148 CRISPR screens. DR ChiTaRS; TTC31; human. DR GenomeRNAi; 64427; -. DR Pharos; Q49AM3; Tdark. DR PRO; PR:Q49AM3; -. DR Proteomes; UP000005640; Chromosome 2. DR RNAct; Q49AM3; protein. DR Bgee; ENSG00000115282; Expressed in sural nerve and 181 other cell types or tissues. DR ExpressionAtlas; Q49AM3; baseline and differential. DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1. DR InterPro; IPR011990; TPR-like_helical_dom_sf. DR InterPro; IPR019734; TPR_rpt. DR PANTHER; PTHR47678; TETRATRICOPEPTIDE REPEAT PROTEIN 31; 1. DR PANTHER; PTHR47678:SF1; TETRATRICOPEPTIDE REPEAT PROTEIN 31; 1. DR Pfam; PF13432; TPR_16; 2. DR SMART; SM00028; TPR; 3. DR SUPFAM; SSF48452; TPR-like; 1. DR PROSITE; PS50005; TPR; 2. DR PROSITE; PS50293; TPR_REGION; 1. PE 1: Evidence at protein level; KW Alternative splicing; Coiled coil; Phosphoprotein; KW Proteomics identification; Reference proteome; Repeat; TPR repeat. FT CHAIN 1..519 FT /note="Tetratricopeptide repeat protein 31" FT /id="PRO_0000281149" FT REPEAT 305..338 FT /note="TPR 1" FT REPEAT 339..372 FT /note="TPR 2" FT REPEAT 373..406 FT /note="TPR 3" FT REGION 175..230 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 253..294 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 474..506 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 147..197 FT /evidence="ECO:0000255" FT COMPBIAS 175..187 FT /note="Basic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 481..490 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 278 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:23186163" FT VAR_SEQ 281..285 FT /note="REERP -> ATSPC (in isoform 2)" FT /evidence="ECO:0000303|PubMed:14702039, FT ECO:0000303|PubMed:15489334" FT /id="VSP_023990" FT VAR_SEQ 286..519 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|PubMed:14702039, FT ECO:0000303|PubMed:15489334" FT /id="VSP_023991" FT VARIANT 28 FT /note="A -> V (in dbSNP:rs6707475)" FT /evidence="ECO:0000269|PubMed:15489334" FT /id="VAR_047357" FT VARIANT 205 FT /note="T -> P (in dbSNP:rs35852562)" FT /id="VAR_047358" FT CONFLICT 141 FT /note="L -> P (in Ref. 1; BAB14272)" FT /evidence="ECO:0000305" FT CONFLICT 216 FT /note="P -> T (in Ref. 3; AAH36017)" FT /evidence="ECO:0000305" FT CONFLICT 413 FT /note="R -> H (in Ref. 3; AAH36017)" FT /evidence="ECO:0000305" SQ SEQUENCE 519 AA; 57105 MW; B618E043E0FBD78E CRC64; MAPIPKTVGR IKLDCSLRPS CPLEVAAAPK LCKEFGPEDY GEEDIVDFLR RLVESDPQGL HRIHVDGSSG RLQLWHHDYL LGHLDDEGKS TGQSDRGKGA EGLGTYCGLR KSFLYPPQES EPCPQSPSAS ATFPSVSDSL LQVAMPQKLL VTEEEANRLA EELVAEEERM KQKAEKKRLK KKRQKERKRQ ERLEQYCGEP KASTTSDGDE SPPSSPGNPV QGQCGEEEDS LDLSSTFVSL ALRKVGDWPL SARREKGLNQ EPQGRGLALQ KMGQEEESPP REERPQQSPK VQASPGLLAA ALQQSQELAK LGTSFAQNGF YHEAVVLFTQ ALKLNPQDHR LFGNRSFCHE RLGQPAWALA DAQVALTLRP GWPRGLFRLG KALMGLQRFR EAAAVFQETL RGGSQPDAAR ELRSCLLHLT LQGQRGGICA PPLSPGALQP LPHAELAPSG LPSLRCPRST ALRSPGLSPL LHYPSCHRSH PNQPLSQTQS RRPHPLKPQD PSKGWDILGL GLQHLSQAR //