ID   DNAE2_PSE14             Reviewed;        1031 AA.
AC   Q48IB1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   04-NOV-2026, entry version 110.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   OrderedLocusNames=PSPPH_2680;
OS   Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)
OS   (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=264730;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1448A / Race 6;
RX   PubMed=16159782; DOI=10.1128/jb.187.18.6488-6498.2005;
RA   Joardar V., Lindeberg M., Jackson R.W., Selengut J., Dodson R.,
RA   Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn Giglio M.,
RA   Madupu R., Nelson W.C., Rosovitz M.J., Sullivan S.A., Crabtree J.,
RA   Creasy T., Davidsen T.M., Haft D.H., Zafar N., Zhou L., Halpin R.,
RA   Holley T., Khouri H.M., Feldblyum T.V., White O., Fraser C.M.,
RA   Chatterjee A.K., Cartinhour S., Schneider D., Mansfield J.W., Collmer A.,
RA   Buell R.;
RT   "Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola
RT   1448A reveals divergence among pathovars in genes involved in virulence and
RT   transposition.";
RL   J. Bacteriol. 187:6488-6498(2005).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) +
CC         diphosphate; Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-
CC         COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112;
CC         EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000058; AAZ34765.1; -; Genomic_DNA.
DR   RefSeq; WP_004667591.1; NC_005773.3.
DR   AlphaFoldDB; Q48IB1; -.
DR   SMR; Q48IB1; -.
DR   KEGG; psp:PSPPH_2680; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; AACQHGT; -.
DR   Proteomes; UP000000551; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-ARBA.
DR   CDD; cd04485; DnaE_OBF; 1.
DR   CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR   FunFam; 1.10.150.870:FF:000002; Error-prone DNA polymerase; 1.
DR   Gene3D; 1.10.150.870; -; 1.
DR   Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DnaE2.
DR   InterPro; IPR004805; DnaE2/DnaE/PolC.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   NCBIfam; TIGR00594; polc; 1.
DR   NCBIfam; NF004225; PRK05672.1; 1.
DR   PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; PHP domain-like; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1031
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070591"
SQ   SEQUENCE   1031 AA;  116205 MW;  A52A908F7A4982A3 CRC64;
     MTSEYAELHC LSNFSFQRGA SSARELFERA SRHGYQALAI TDECTLAGIV RAWQASISTG
     LPLIIGSEMH IENGPKAVLL VESQTGYEAL CKLITVARRR ARKGEYRLLR EDFEPSSDGL
     LAIWLPDIEG DAQACLAHGR WLRERFAERL WLGVELHRGA DDEQRLADLL ALAQSLGIPA
     VASGDVHMHA RGRRALQDTM TAIRHHTTVA EAGHLLFANG ERHLRPLDAL AEHYPDWLLA
     ESVRIARRCT FDLKQLKYEY PHELVPKGQT PTSWLRELTE RGARKRWPNG LTPATRAQVE
     KELALITEKK FDSYFLTVHD IVEFARSQHI LCQGRGSAAN SAVCYALGIT ELNPEKSNLL
     FERFISRERN EPPDIDVDFE HDRREEVIQY IFRRYGRGRA ALTAVASTYH GSGAMRDVAK
     VLGLPPDQIN ALAEAFSRWS DSLPSPERLR EYGFDADTPI LKRVLALTGE LIGFPRHLSQ
     HPGGFVISEH PLETLVPVEN AAMADRTIIQ WDKDDLDLVG LLKVDILALG MLSALRRTFD
     LVHLHRGKRW TLADLPGDDR ETYEMISRAD TIGVFQIESR AQMAMLPRLR PEKFYDLVIE
     VAIVRPGPIQ GDMVHPYLRR RNKEEAITYP PKLKSVFERT LGVPLFQEQV MEVAIIAAGY
     TPGEADELRR AMAAWKRHGG LEPHRERLRT GMLKNGYEAD FADRIFEQIK GFGSYGFPES
     HAASFALLTY ASCWLKCHEP AAFTCALINS WPMGFYSPDQ LLQDARRHHI EIRPVDVRYS
     DWDCSLEPLD HPDSTRNLAI RLGLRMVRGF REDDARRIET ARSKRLFSDA TDLTLRAGLD
     ARAAEALADS GALRGLIGHR HRARWEVAGV EAQRPLFNDL PSEDTQVTLP LPTVAEDLMA
     DYATLGTTLG PHPLALLRRQ LAAKRFRSSQ DLLSLENDRT LSVAGLVIGR QRPGTASGVT
     FVTLEDEFGM VNVVVWRDLA ERQRKVLVGS QLLQVFGRLE SKSGVRHLIA QRLYDLTPLL
     TGLDVRSRDF Q
//