ID   NAC_ECOLI               Reviewed;         305 AA.
AC   Q47005; Q47082;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   04-NOV-2026, entry version 161.
DE   RecName: Full=Nitrogen assimilation regulatory protein nac;
DE   AltName: Full=Nitrogen assimilation control protein;
GN   Name=nac; OrderedLocusNames=b1988, JW1967;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=9495755; DOI=10.1128/jb.180.5.1166-1173.1998;
RA   Muse W.B., Bender R.A.;
RT   "The nac (nitrogen assimilation control) gene from Escherichia coli.";
RL   J. Bacteriol. 180:1166-1173(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 245-305.
RC   STRAIN=K12;
RX   PubMed=8529872; DOI=10.1016/0378-1119(95)00606-8;
RA   Iwanicka-Nowicka R., Hryniewicz M.M.;
RT   "A new gene, cbl, encoding a member of the LysR family of transcriptional
RT   regulators belongs to Escherichia coli cys regulon.";
RL   Gene 166:11-17(1995).
CC   -!- FUNCTION: Transcriptional activator for the hut, put and ure operons
CC       and repressor for the gdh and gltB operons in response to nitrogen
CC       limitation. Negative regulator of its own expression (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; U56736; AAD14869.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75050.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15806.1; -; Genomic_DNA.
DR   EMBL; L31639; AAA99928.1; -; Genomic_DNA.
DR   PIR; D64963; D64963.
DR   RefSeq; NP_416493.1; NC_000913.3.
DR   RefSeq; WP_001011462.1; NZ_CP009789.1.
DR   SMR; Q47005; -.
DR   BioGRID; 4260407; 99.
DR   IntAct; Q47005; 1.
DR   STRING; 511145.b1988; -.
DR   PaxDb; 511145-b1988; -.
DR   DNASU; 946501; -.
DR   EnsemblBacteria; AAC75050; AAC75050; b1988.
DR   GeneID; 946501; -.
DR   KEGG; ecj:JW1967; -.
DR   KEGG; eco:b1988; -.
DR   KEGG; ecoc:C3026_11220; -.
DR   PATRIC; fig|1411691.4.peg.265; -.
DR   EchoBASE; EB4013; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_6_5_6; -.
DR   InParanoid; Q47005; -.
DR   OMA; AATGMQT; -.
DR   OrthoDB; 8479357at2; -.
DR   PhylomeDB; Q47005; -.
DR   BioCyc; EcoCyc:G7072-MONOMER; -.
DR   PRO; PR:Q47005; -.
DR   AbasyAtlas; Q47005; -.
DR   CDD; cd08433; PBP2_Nac; 1.
DR   FunFam; 1.10.10.10:FF:000001; LysR family transcriptional regulator; 1.
DR   FunFam; 3.40.190.290:FF:000016; Nitrogen assimilation regulatory protein nac; 1.
DR   Gene3D; 3.40.190.290; -; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000847; LysR_HTH_N.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR30293:SF0; NITROGEN ASSIMILATION REGULATORY PROTEIN NAC; 1.
DR   PANTHER; PTHR30293; TRANSCRIPTIONAL REGULATORY PROTEIN NAC-RELATED; 1.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF53850; Periplasmic binding protein-like II; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   NCBIfam; NF008410; nac; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:EcoCyc.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IDA:EcoCyc.
DR   GO; GO:2000142; P:regulation of DNA-templated transcription initiation; IBA:GO_Central.
DR   Proteomes; UP000000625; Chromosome.
DR   AlphaFoldDB; Q47005; -.
DR   FunCoup; Q47005; 525.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Nitrate assimilation; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..305
FT                   /note="Nitrogen assimilation regulatory protein nac"
FT                   /id="PRO_0000105686"
FT   DOMAIN          1..58
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        18..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   305 AA;  32835 MW;  B0B5DC408C6C6821 CRC64;
     MNFRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GELNQQLLIR TKRGVTPTDA
     GKILYTHARA ILRQCEQAQL AVHNVGQALS GQVSIGFAPG TAASSITMPL LQAVRAEFPE
     IVIYLHENSG AVLNEKLINH QLDMAVIYEH SPVAGVSSQA LLKEDLFLVG TQDCPGQSVD
     VNAIAQMNLF LPSDYSAIRL RVDEAFSLRR LTAKVIGEIE SIATLTAAIA SGMGVAVLPE
     SAARSLCGAV NGWMSRITTP SMSLSLSLNL PARANLSPQA QAVKELLMSV ISSPVMEKRQ
     WQLVS
//