ID   TRUB_METBF              Reviewed;         338 AA.
AC   Q46GC3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   18-JUN-2025, entry version 126.
DE   RecName: Full=Probable tRNA pseudouridine synthase B {ECO:0000255|HAMAP-Rule:MF_01081};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA pseudouridine(55) synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE            Short=Psi55 synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA pseudouridylate synthase {ECO:0000255|HAMAP-Rule:MF_01081};
DE   AltName: Full=tRNA-uridine isomerase {ECO:0000255|HAMAP-Rule:MF_01081};
GN   Name=truB {ECO:0000255|HAMAP-Rule:MF_01081};
GN   OrderedLocusNames=Mbar_A0082;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC   Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
CC   -!- FUNCTION: Could be responsible for synthesis of pseudouridine from
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01081}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01081};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family. Type 2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01081}.
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DR   EMBL; CP000099; AAZ69069.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q46GC3; -.
DR   SMR; Q46GC3; -.
DR   STRING; 269797.Mbar_A0082; -.
DR   PaxDb; 269797-Mbar_A0082; -.
DR   KEGG; mba:Mbar_A0082; -.
DR   eggNOG; arCOG00987; Archaea.
DR   HOGENOM; CLU_032087_3_0_2; -.
DR   OrthoDB; 35866at2157; -.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0160148; F:tRNA pseudouridine(55) synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000495; P:box H/ACA sno(s)RNA 3'-end processing; IEA:TreeGrafter.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IEA:TreeGrafter.
DR   GO; GO:0031118; P:rRNA pseudouridine synthesis; IEA:TreeGrafter.
DR   GO; GO:0031120; P:snRNA pseudouridine synthesis; IEA:TreeGrafter.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd02572; PseudoU_synth_hDyskerin; 1.
DR   CDD; cd21148; PUA_Cbf5; 1.
DR   FunFam; 3.30.2350.10:FF:000001; H/ACA ribonucleoprotein complex subunit CBF5; 1.
DR   Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR   Gene3D; 2.30.130.10; PUA domain; 1.
DR   HAMAP; MF_01081; TruB_arch; 1.
DR   InterPro; IPR012960; Dyskerin-like.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   InterPro; IPR026326; TruB_arch.
DR   InterPro; IPR032819; TruB_C.
DR   NCBIfam; TIGR00425; CBF5; 1.
DR   NCBIfam; NF003280; PRK04270.1; 1.
DR   PANTHER; PTHR23127; CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5; 1.
DR   PANTHER; PTHR23127:SF0; H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1; 1.
DR   Pfam; PF08068; DKCLD; 1.
DR   Pfam; PF01472; PUA; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SMART; SM01136; DKCLD; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   SUPFAM; SSF88697; PUA domain-like; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   3: Inferred from homology;
KW   Isomerase; tRNA processing.
FT   CHAIN           1..338
FT                   /note="Probable tRNA pseudouridine synthase B"
FT                   /id="PRO_1000084721"
FT   DOMAIN          245..320
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
FT   ACT_SITE        78
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01081"
SQ   SEQUENCE   338 AA;  36888 MW;  A346867FC9192D06 CRC64;
     MSPAGKLPSE AERILVRKSG AWTNPSYGSY PEKRPILEYI EKGVVNIDKP KGPTSHEVAA
     WVKAILGVST AGHAGSLDPK VTGLLPTLLG KATKAVPALR LSGKEYVCLL KLHKEMPQKL
     VRKVCEEFTG PIYQMPPIKS AVKRVIRIRT IYYLEVLEIE GSFVLFRVGC EAGTYIRKLC
     HDIGLALGCG GHMQELRRTK AGPFTEETLV TLQDLKDAYV LWKEDGDESE IRRVIMPMET
     AVSHLPKIIL RDSAVDAICS GAALAVPGIT SLDANLKKGE LIALFTLKGE LVALAKAEMS
     TEELLKASTG LAATSVRIMM EIGTYPKGWT KKEYGVES
//