ID   KIZ_MOUSE               Reviewed;         695 AA.
AC   Q3UXL4; A2AUS7; B2RX19;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   02-SEP-2026, entry version 126.
DE   RecName: Full=Centrosomal protein kizuna;
DE   AltName: Full=Polo-like kinase 1 substrate 1;
GN   Name=Kiz; Synonyms=Gm114, Plk1s1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Muellerian duct;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PROTEIN SEQUENCE OF 73-82; 76-87 AND 132-158, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   STRAIN=OF1; TISSUE=Hippocampus;
RA   Lubec G., Sunyer B., Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-667 AND SER-670, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Centrosomal protein required for establishing a robust
CC       mitotic centrosome architecture that can endure the forces that
CC       converge on the centrosomes during spindle formation. Required for
CC       stabilizing the expanded pericentriolar material around the centriole.
CC       {ECO:0000250|UniProtKB:Q2M2Z5}.
CC   -!- SUBUNIT: Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.
CC       {ECO:0000250|UniProtKB:Q2M2Z5}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome {ECO:0000250|UniProtKB:Q2M2Z5}. Cytoplasm,
CC       cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q2M2Z5}.
CC       Note=Localizes to centrosomes throughout the cell cycle. After
CC       centrosome duplication, it usually remains associated only with the
CC       mother centrosome, containing the older mature centriole and particles
CC       surrounding it. During prophase, additional particles accumulate around
CC       both separating centrosomes. Does not accumulate at the microtubule
CC       minus ends, but instead localizes to the centrosomes and
CC       centrosome- surrounding area in a microtubule-independent and dependent
CC       manner, respectively. {ECO:0000250|UniProtKB:Q2M2Z5}.
CC   -!- PTM: Phosphorylation at Thr-391 by PLK1 is not needed for centrosomal
CC       localization or pericentriolar material expansion but is indispensable
CC       for spindle-pole stabilization. {ECO:0000250|UniProtKB:Q2M2Z5}.
CC   -!- SIMILARITY: Belongs to the kizuna family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAM27275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AK135486; BAE22549.1; -; mRNA.
DR   EMBL; AL929215; CAM27275.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL929215; CAM27276.1; -; Genomic_DNA.
DR   EMBL; BC150844; AAI50845.1; -; mRNA.
DR   EMBL; BC150845; AAI50846.1; -; mRNA.
DR   CCDS; CCDS16832.1; -.
DR   RefSeq; NP_001028470.1; NM_001033298.4.
DR   AlphaFoldDB; Q3UXL4; -.
DR   SMR; Q3UXL4; -.
DR   FunCoup; Q3UXL4; 787.
DR   IntAct; Q3UXL4; 1.
DR   STRING; 10090.ENSMUSP00000096884; -.
DR   iPTMnet; Q3UXL4; -.
DR   PhosphoSitePlus; Q3UXL4; -.
DR   jPOST; Q3UXL4; -.
DR   PaxDb; 10090-ENSMUSP00000096884; -.
DR   PeptideAtlas; Q3UXL4; -.
DR   ProteomicsDB; 263446; -.
DR   Pumba; Q3UXL4; -.
DR   Antibodypedia; 24776; 130 antibodies from 26 providers.
DR   Ensembl; ENSMUST00000099278.9; ENSMUSP00000096884.3; ENSMUSG00000074749.11.
DR   GeneID; 228730; -.
DR   KEGG; mmu:228730; -.
DR   UCSC; uc008mso.1; mouse.
DR   AGR; MGI:2684960; -.
DR   CTD; 55857; -.
DR   MGI; MGI:2684960; Kiz.
DR   VEuPathDB; HostDB:ENSMUSG00000074749; -.
DR   eggNOG; ENOG502R72X; Eukaryota.
DR   GeneTree; ENSGT00390000010121; -.
DR   HOGENOM; CLU_026235_0_0_1; -.
DR   InParanoid; Q3UXL4; -.
DR   OMA; EKEQTHC; -.
DR   OrthoDB; 8015657at2759; -.
DR   PhylomeDB; Q3UXL4; -.
DR   BioGRID-ORCS; 228730; 3 hits in 78 CRISPR screens.
DR   PRO; PR:Q3UXL4; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q3UXL4; protein.
DR   Bgee; ENSMUSG00000074749; Expressed in interventricular septum and 239 other cell types or tissues.
DR   ExpressionAtlas; Q3UXL4; baseline and differential.
DR   GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0019901; F:protein kinase binding; ISO:GO_Central.
DR   GO; GO:0007051; P:spindle organization; ISS:UniProtKB.
DR   InterPro; IPR026742; Centrosomal_kizuma.
DR   InterPro; IPR060349; Kizuna_N.
DR   PANTHER; PTHR16299; CENTROSOMAL PROTEIN KIZUNA; 1.
DR   PANTHER; PTHR16299:SF2; CENTROSOMAL PROTEIN KIZUNA; 1.
DR   Pfam; PF28416; Kizuna_N; 1.
PE   1: Evidence at protein level;
KW   Cell projection; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Direct protein sequencing; Phosphoprotein; Reference proteome.
FT   CHAIN           1..695
FT                   /note="Centrosomal protein kizuna"
FT                   /id="PRO_0000301852"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          261..313
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          351..391
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          444..465
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          633..695
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          29..57
FT                   /evidence="ECO:0000255"
FT   COILED          102..132
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..12
FT                   /note="Gly residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..24
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        263..274
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        282..297
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        299..313
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        360..377
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        382..391
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        448..465
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        633..645
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        676..686
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         391
FT                   /note="Phosphothreonine; by PLK1"
FT                   /evidence="ECO:0000250|UniProtKB:Q2M2Z5"
FT   MOD_RES         667
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         670
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         672
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q2M2Z5"
SQ   SEQUENCE   695 AA;  76542 MW;  71DDC7CB94FD6EBF CRC64;
     MPRGRGGGGG GLRQASATSA PLASPSYYER VGQLQQALRD SEKKRLDLED KLYEYNKSDK
     CRAKLKCAKL KKYLKEVCES ERRAQVRNQG YLKKFECVQT YVEHLTTNTE KLQKLKTEYE
     AEVKRMRLLS KDSLGMSDED GAKDAVQAGI NSGTAMSRGL YQPATIFMGR QMSAISSIED
     FSTELKSNQP TKNFSISDPH SHQQTAQSSC VTDSCVVQTN GDTQCLNKSD KIHGKTSLQT
     GEKAPVTSYV LSAEEQTHCL EIGSSTQHSK SNLSEGRKSA ELHSSLQERL SPENSITDLK
     CDSSSRSEGS DREILTQEHI EVREERAGPL VPMMAASEHC TSAKKWAGEK HSAWEASSDD
     LDHGDSKSQK AVLKHEEEQE EGSSCSSSDL TVSVSEDDLI LESLAALSNP GAKMAGKDGM
     QALRAAHTEP RQDSLFTEYV LQTQSFPDSK REPSPDSPRQ PEKVPDCHLL KTRGQCMKEH
     DNSLKEEATT LLKKVLTEEC DHRSAIHSNE SSCSMPSILN DNNGIKEAKP ALRLNSVLTR
     EQEVSSGCGD ESKEESIAAA PGTGHREADL VPSELFQFSL GAAMKETKAY QLLKKSTLQD
     NSNQAEERFE SQFSGLDIGG SMFTTKTAHK IASEASFSSS EGSPLSRHES KRDPVTTIKS
     NAFWGESDDS NSEIEAALRP RDHDMPDDFD DFYDT
//