ID   Q3SPD5_NITWN            Unreviewed;       376 AA.
AC   Q3SPD5;
DT   11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT   11-OCT-2005, sequence version 1.
DT   02-SEP-2026, entry version 114.
DE   RecName: Full=Methyltransferase {ECO:0000256|RuleBase:RU362026};
DE            EC=2.1.1.- {ECO:0000256|RuleBase:RU362026};
GN   OrderedLocusNames=Nwi_2603 {ECO:0000313|EMBL:ABA05856.1};
OS   Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 /
OS   NCIMB 11846 / Nb-255).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Nitrobacteraceae; Nitrobacter.
OX   NCBI_TaxID=323098 {ECO:0000313|EMBL:ABA05856.1, ECO:0000313|Proteomes:UP000002531};
RN   [1] {ECO:0000313|EMBL:ABA05856.1, ECO:0000313|Proteomes:UP000002531}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255
RC   {ECO:0000313|Proteomes:UP000002531};
RX   PubMed=16517654; DOI=10.1128/AEM.72.3.2050-2063.2006;
RA   Starkenburg S.R., Chain P.S., Sayavedra-Soto L.A., Hauser L., Land M.L.,
RA   Larimer F.W., Malfatti S.A., Klotz M.G., Bottomley P.J., Arp D.J.,
RA   Hickey W.J.;
RT   "Genome sequence of the chemolithoautotrophic nitrite-oxidizing bacterium
RT   Nitrobacter winogradskyi Nb-255.";
RL   Appl. Environ. Microbiol. 72:2050-2063(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine +
CC         H(+); Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00047942};
CC   -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC       {ECO:0000256|ARBA:ARBA00006594, ECO:0000256|RuleBase:RU362026}.
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DR   EMBL; CP000115; ABA05856.1; -; Genomic_DNA.
DR   RefSeq; WP_011315806.1; NC_007406.1.
DR   AlphaFoldDB; Q3SPD5; -.
DR   STRING; 323098.Nwi_2603; -.
DR   REBASE; 11322; M.NwiORF2603P.
DR   KEGG; nwi:Nwi_2603; -.
DR   eggNOG; COG2189; Bacteria.
DR   HOGENOM; CLU_024927_5_1_5; -.
DR   OrthoDB; 9800801at2; -.
DR   Proteomes; UP000002531; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   FunFam; 3.40.50.150:FF:000276; Methyltransferase; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR002941; DNA_methylase_N4/N6.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR040843; RAMA.
DR   InterPro; IPR001091; RM_Methyltransferase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR   PANTHER; PTHR13370:SF3; TRNA (GUANINE(10)-N2)-METHYLTRANSFERASE HOMOLOG; 1.
DR   Pfam; PF01555; N6_N4_Mtase; 1.
DR   Pfam; PF18755; RAMA; 1.
DR   PRINTS; PR00508; S21N4MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   3: Inferred from homology;
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:ABA05856.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002531};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          42..264
FT                   /note="DNA methylase N-4/N-6"
FT                   /evidence="ECO:0000259|Pfam:PF01555"
FT   DOMAIN          281..374
FT                   /note="RAMA"
FT                   /evidence="ECO:0000259|Pfam:PF18755"
SQ   SEQUENCE   376 AA;  41490 MW;  40AAF3C35A50D522 CRC64;
     MGVSRRGASA RAPRTKFDSP VSRIIIGDCV AGMSKLQAGS ADLIFADPPY NLQLKGDLKR
     PDESHVDAVN SDWDKFSSFS AYDEFTRAWL LACRRVMKPS ATIWVIGSYH NIFRVGAIMQ
     DLGFWVLNDI VWRKTNPMPN FRGRRFTNAH ETMIWAARDE NAKGYTFNYE ALKAANEDVQ
     ARSDWLIPLC TGNERLKDED GRKVHPTQKP EGLLARVLLS SSKPGDLVID PFNGTGTTGA
     VAKRLGRNYI GFERDRTYAA AAEKRIAAVE PLPEATLAPF MTAREAPRVA FSELIERGMI
     SPGAKLVDAK KRHGALVRAD GAIMLGDKVG SIHRIGAVAQ GAGACNGWTF WHIETSKGLK
     LIDELRAEIR SQMASD
//