ID   RLMF_PSET1              Reviewed;         310 AA.
AC   Q3IBW7;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 2.
DT   04-NOV-2026, entry version 93.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase F {ECO:0000255|HAMAP-Rule:MF_01848};
DE            EC=2.1.1.181 {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=23S rRNA mA1618 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=rRNA adenine N-6-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
GN   Name=rlmF {ECO:0000255|HAMAP-Rule:MF_01848};
GN   OrderedLocusNames=PSHAb0296;
OS   Pseudoalteromonas translucida (strain TAC 125).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=326442;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TAC 125;
RX   PubMed=16169927; DOI=10.1101/gr.4126905;
RA   Medigue C., Krin E., Pascal G., Barbe V., Bernsel A., Bertin P.N.,
RA   Cheung F., Cruveiller S., D'Amico S., Duilio A., Fang G., Feller G., Ho C.,
RA   Mangenot S., Marino G., Nilsson J., Parrilli E., Rocha E.P.C., Rouy Z.,
RA   Sekowska A., Tutino M.L., Vallenet D., von Heijne G., Danchin A.;
RT   "Coping with cold: the genome of the versatile marine Antarctica bacterium
RT   Pseudoalteromonas haloplanktis TAC125.";
RL   Genome Res. 15:1325-1335(2005).
CC   -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = N(6)-
CC         methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine + H(+);
CC         Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01848};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC       family. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAI89337.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR954247; CAI89337.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q3IBW7; -.
DR   SMR; Q3IBW7; -.
DR   STRING; 326442.PSHAb0296; -.
DR   KEGG; pha:PSHAb0296; -.
DR   PATRIC; fig|326442.8.peg.3205; -.
DR   eggNOG; COG3129; Bacteria.
DR   HOGENOM; CLU_027534_3_0_6; -.
DR   OMA; AAELWCQ; -.
DR   BioCyc; PHAL326442:PSHA_RS16275-MONOMER; -.
DR   Proteomes; UP000006843; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070475; P:rRNA base methylation; IEA:TreeGrafter.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR   InterPro; IPR010286; METTL16/RlmF.
DR   InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; NF008725; rlmF; 1.
DR   PANTHER; PTHR13393:SF0; RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16; 1.
DR   PANTHER; PTHR13393; SAM-DEPENDENT METHYLTRANSFERASE; 1.
DR   Pfam; PF05971; Methyltransf_10; 1.
DR   PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..310
FT                   /note="Ribosomal RNA large subunit methyltransferase F"
FT                   /id="PRO_0000349924"
SQ   SEQUENCE   310 AA;  34702 MW;  6C81F555D8294349 CRC64;
     MKASTSRAKL HPRNQHLNGY DLDLLTEQHP ALSAFIITTP AGTKSIDFSD NKAVKTLNQA
     LLKAHYNVDF WDIPQHNLCP PIPGRVDYIH YLADLLADDN QTQIPTGRQV KVLDIGTGAN
     LVYPLTGGHE YNWHFTGTDI DPAAIKVAKQ IAQFNNLKIT LKQQKNAANI FKGVINSKDL
     YHLTLCNPPF HASSEDAVKG SERKWKNLGK APSSTLNFGG QNNELWCEGG EVQFISNMIK
     ESAAVAEQVM WFTSLVSKKD NIAALEQELK QHPIAQYKIV DMAQGQKVSR FIAWSYFDKA
     TREQICEELE
//