ID   PMT5_ARATH              Reviewed;         606 AA.
AC   Q3EC77; Q8H0Z7; Q94BV9; Q9ZQ84;
DT   23-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   02-SEP-2026, entry version 130.
DE   RecName: Full=Probable methyltransferase PMT5;
DE            EC=2.1.1.-;
GN   OrderedLocusNames=At2g03480; ORFNames=T4M8.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=17461780; DOI=10.1111/j.1365-313x.2007.03123.x;
RA   Krupkova E., Immerzeel P., Pauly M., Schmulling T.;
RT   "The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative
RT   methyltransferase is required for cell adhesion and co-ordinated plant
RT   development.";
RL   Plant J. 50:735-750(2007).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC       Single-pass type II membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q3EC77-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q3EC77-2; Sequence=VSP_038902;
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD17428.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAK63953.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC       Sequence=AAN46794.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR   EMBL; AC006284; AAD17428.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC05705.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC05706.1; -; Genomic_DNA.
DR   EMBL; OZ408684; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AY039849; AAK63953.1; ALT_SEQ; mRNA.
DR   EMBL; BT001040; AAN46794.1; ALT_SEQ; mRNA.
DR   PIR; A84449; A84449.
DR   RefSeq; NP_027543.2; NM_126399.3. [Q3EC77-1]
DR   RefSeq; NP_973410.1; NM_201681.2. [Q3EC77-2]
DR   AlphaFoldDB; Q3EC77; -.
DR   SMR; Q3EC77; -.
DR   FunCoup; Q3EC77; 293.
DR   STRING; 3702.Q3EC77; -.
DR   GlyGen; Q3EC77; 2 sites.
DR   iPTMnet; Q3EC77; -.
DR   PaxDb; 3702-Q3EC77; -.
DR   ProteomicsDB; 234984; -. [Q3EC77-1]
DR   GeneID; 814877; -.
DR   KEGG; ath:AT2G03480; -.
DR   Araport; AT2G03480; -.
DR   TAIR; AT2G03480; QUL2.
DR   eggNOG; ENOG502QT31; Eukaryota.
DR   InParanoid; Q3EC77; -.
DR   OMA; TWVPENY; -.
DR   PhylomeDB; Q3EC77; -.
DR   PRO; PR:Q3EC77; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q3EC77; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR   GO; GO:0000138; C:Golgi trans cisterna; HDA:TAIR.
DR   GO; GO:0005739; C:mitochondrion; ISM:TAIR.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:InterPro.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   FunFam; 3.40.50.150:FF:000119; probable pectin methyltransferase QUA2; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR004159; Put_SAM_MeTrfase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR10108:SF1083; METHYLTRANSFERASE PMT4-RELATED; 1.
DR   PANTHER; PTHR10108; SAM-DEPENDENT METHYLTRANSFERASE; 1.
DR   Pfam; PF03141; Methyltransf_29; 2.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 2.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Endoplasmic reticulum; Glycoprotein; Membrane;
KW   Methyltransferase; Reference proteome; Signal-anchor; Transferase;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..606
FT                   /note="Probable methyltransferase PMT5"
FT                   /id="PRO_0000393245"
FT   TOPO_DOM        1..20
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        21..41
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        42..606
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        101
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        409
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         402..426
FT                   /note="KRWISIQNRSAVAGTTSAGLEIHGK -> LKPEEFFEDTQIWR (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_038902"
SQ   SEQUENCE   606 AA;  67819 MW;  B2797F86E6B05EBB CRC64;
     MRGSWYKSVS SVFGLRPRIR GLLFFIVGVV ALVTILAPLT SNSYDSSSSS TLVPNIYSNY
     RRIKEQAAVD YLDLRSLSLG ASLKEFPFCG KERESYVPCY NITGNLLAGL QEGEELDRHC
     EFEREKERCV VRPPRDYKIP LRWPLGRDII WSGNVKITKD QFLSSGTVTT RLMLLEENQI
     TFHSEDGLVF DGVKDYARQI AEMIGLGSDT EFAQAGVRTV LDIGCGFGSF GAHLVSLKLM
     PICIAEYEAT GSQVQLALER GLPAMIGNFF SKQLPYPALS FDMVHCAQCG TTWDIKDAML
     LLEVDRVLKP GGYFVLTSPT NKAQGNLPDT KKTSISTRVN ELSKKICWSL TAQQDETFLW
     QKTSDSSCYS SRSQASIPLC KDGDSVPYYH PLVPCISGTT SKRWISIQNR SAVAGTTSAG
     LEIHGKSALK NYWSLLTPLI FSDHPKRPGD EDPLPPFNMI RNVMDMHARF GNLNAALLDE
     GKSAWVMNVV PVNARNTLPI ILDRGFAGVL HDWCEPFPTY PRTYDMLHAN ELLTHLSSER
     CSLMDLFLEM DRILRPEGWV VLSDKVGVIE MARALAARVR WEARVIDLQD GSDQRLLVCQ
     KPFIKK
//