ID   ACSF_PROM9              Reviewed;         390 AA.
AC   Q31AS9;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   04-NOV-2026, entry version 102.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN   Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840};
GN   OrderedLocusNames=PMT9312_0956;
OS   Prochlorococcus marinus (strain MIT 9312).
OC   Bacteria; Bacillati; Cyanobacteriota; Cyanophyceae; Synechococcales;
OC   Prochlorococcaceae; Prochlorococcus.
OX   NCBI_TaxID=74546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MIT 9312;
RX   PubMed=16556843; DOI=10.1126/science.1122050;
RA   Coleman M.L., Sullivan M.B., Martiny A.C., Steglich C., Barry K.,
RA   Delong E.F., Chisholm S.W.;
RT   "Genomic islands and the ecology and evolution of Prochlorococcus.";
RL   Science 311:1768-1770(2006).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis. Mediates the cyclase reaction, which results in the
CC       formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC       chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC       (MgPMME). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 O2 + 2
CC         H(+) = 3,8-divinyl protochlorophyllide a + 3 NADP(+) + 5 H2O;
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01840};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis (light-independent). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01840}.
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DR   EMBL; CP000111; ABB50016.1; -; Genomic_DNA.
DR   RefSeq; WP_011376508.1; NC_007577.1.
DR   AlphaFoldDB; Q31AS9; -.
DR   STRING; 74546.PMT9312_0956; -.
DR   KEGG; pmi:PMT9312_0956; -.
DR   eggNOG; COG1633; Bacteria.
DR   HOGENOM; CLU_048037_0_0_3; -.
DR   OMA; AAMHHHR; -.
DR   OrthoDB; 141643at2; -.
DR   UniPathway; UPA00670; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0036068; P:light-independent chlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rr_diiron-bd_dom.
DR   NCBIfam; TIGR02029; AcsF; 1.
DR   NCBIfam; NF010172; PRK13654.1; 1.
DR   PANTHER; PTHR31053; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR31053:SF2; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; Ferritin-like; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Iron; Metal-binding; NADP; Oxidoreductase;
KW   Photosynthesis.
FT   CHAIN           1..390
FT                   /note="Magnesium-protoporphyrin IX monomethyl ester
FT                   [oxidative] cyclase"
FT                   /id="PRO_1000070548"
SQ   SEQUENCE   390 AA;  45810 MW;  8013C443F31AF3B2 CRC64;
     MSQSIIESKN KKDVNNGKIP AKETILSPRF YTTDFEAMEN MDLSINEEEL EAICEEFRKD
     YNRHHFVRNS EFDGAAEKLD PETRELFVDF LEGSCTSEFS GFLLYKELSR RIKDKNPLLA
     ECFAHMARDE ARHAGFLNKS MSDFGLQLDL GFLTANKDYT YFPPRSIFYA TYLSEKIGYW
     RYIAIYRHLE KNPDSKIFPL FNYFENWCQD ENRHGDFFDA LMKAQPRTVK SLSQKINIGG
     TTFTHPLFDY FHRFRYFLNN LPITSKLWSR FFLLAVFATM YARDLGIKKD FYSSLGLDAR
     EYDQYVINKT NETSARVFPV VLDVYDKSFY GRLDKIVENN KVLSNIANSE ENKVSKTLKK
     LPTYLSNGYQ LLRLYLLKPL DSKDFQPSIR
//