ID   Q2T433_BURTA            Unreviewed;       509 AA.
AC   Q2T433;
DT   24-JAN-2006, integrated into UniProtKB/TrEMBL.
DT   24-JAN-2006, sequence version 1.
DT   02-SEP-2026, entry version 84.
DE   SubName: Full=Levansucrase {ECO:0000313|EMBL:ABC36154.1};
GN   OrderedLocusNames=BTH_II1872 {ECO:0000313|EMBL:ABC36154.1};
OS   Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CCUG 48851 /
OS   CIP 106301 / E264).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC   Burkholderiales; Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=271848 {ECO:0000313|EMBL:ABC36154.1, ECO:0000313|Proteomes:UP000001930};
RN   [1] {ECO:0000313|EMBL:ABC36154.1, ECO:0000313|Proteomes:UP000001930}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700388 / DSM 13276 / CCUG 48851 / CIP 106301 / E264
RC   {ECO:0000313|Proteomes:UP000001930};
RX   PubMed=16336651; DOI=10.1186/1471-2164-6-174;
RA   Kim H.S., Schell M.A., Yu Y., Ulrich R.L., Sarria S.H., Nierman W.C.,
RA   DeShazer D.;
RT   "Bacterial genome adaptation to niches: divergence of the potential
RT   virulence genes in three Burkholderia species of different survival
RT   strategies.";
RL   BMC Genomics 6:174-174(2005).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR603469-3};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family.
CC       {ECO:0000256|ARBA:ARBA00006775, ECO:0000256|RuleBase:RU361220}.
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DR   EMBL; CP000085; ABC36154.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2T433; -.
DR   KEGG; bte:BTH_II1872; -.
DR   HOGENOM; CLU_031862_1_0_4; -.
DR   Proteomes; UP000001930; Chromosome II.
DR   GO; GO:0050053; F:levansucrase activity; IEA:InterPro.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; Glycosyl hydrolase domain, family 43; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR603469-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR603469-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001930};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           21..509
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004216317"
FT   ACT_SITE        93
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-1"
FT   ACT_SITE        358
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-1"
FT   BINDING         92
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-2"
FT   BINDING         183
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-2"
FT   BINDING         265..266
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-2"
FT   BINDING         327
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-3"
FT   BINDING         355
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-3"
FT   BINDING         356..358
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-2"
FT   SITE            266
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603469-4"
SQ   SEQUENCE   509 AA;  54778 MW;  EBF62A9D8728227A CRC64;
     MASSAAVTAL AATACLSAHA QSYGAGPAPT PHTQQAYDPE SNFTMRWTRA DMRQIVKQSH
     TAGADKNSLP LALSMPDIAQ NFPLINSNVW VWDTWPLADM RANQLSYKGW EVIFSLSADP
     HAGYTFDDRH VHARIGFFYR RAGIPASQRP ANGGWTWGGH LFPDGASAKV FGTAPMTNNA
     EWSGSARLTH GDNVSLYYTA LSFNRSAPGG ADITPPIAII TRADGHIHAD DKHVWFSGFD
     DHKALLQPDG KMYQTGQQNT YYSFRDPFVF TDPAHPGKTY MVFEGNTGGP RGARTCTEAD
     LGYAPNDPYR EDLNAVMNMG AVYQKANVGL AIATNPQLTE WKFLPPLLSA NCVDDQTERP
     QIYLKDGKYY LFTISHRTTM AAGVDGPDGV YGFVGNGIRS DFLPLNGGSG LVLGNPTDFS
     APAGAPYAQD PNQNPRAFQS YSHYVMPGGR VESFIDAIGA RRGGSLAPTV KIDINGESTT
     VDRAYGVGGL GGYGDIPANQ PAVGAGHND
//