ID   Q2NES1_METST            Unreviewed;       298 AA.
AC   Q2NES1;
DT   07-FEB-2006, integrated into UniProtKB/TrEMBL.
DT   07-FEB-2006, sequence version 1.
DT   02-SEP-2026, entry version 85.
DE   RecName: Full=Coenzyme F420 hydrogenase subunit beta {ECO:0000256|NCBIfam:TIGR03289};
DE            EC=1.12.98.1 {ECO:0000256|NCBIfam:TIGR03289};
GN   Name=frhB {ECO:0000313|EMBL:ABC57682.1};
GN   OrderedLocusNames=Msp_1305 {ECO:0000313|EMBL:ABC57682.1};
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Methanobacteriati; Methanobacteriota; Methanomada group;
OC   Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860 {ECO:0000313|EMBL:ABC57682.1, ECO:0000313|Proteomes:UP000001931};
RN   [1] {ECO:0000313|EMBL:ABC57682.1, ECO:0000313|Proteomes:UP000001931}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3
RC   {ECO:0000313|Proteomes:UP000001931};
RX   PubMed=16385054; DOI=10.1128/JB.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- SIMILARITY: Belongs to the FrhB family.
CC       {ECO:0000256|ARBA:ARBA00038369}.
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DR   EMBL; CP000102; ABC57682.1; -; Genomic_DNA.
DR   RefSeq; WP_011406881.1; NC_007681.1.
DR   AlphaFoldDB; Q2NES1; -.
DR   STRING; 339860.Msp_1305; -.
DR   GeneID; 3856138; -.
DR   GeneID; 41325875; -.
DR   KEGG; mst:Msp_1305; -.
DR   eggNOG; arCOG02651; Archaea.
DR   HOGENOM; CLU_037958_0_0_2; -.
DR   OrthoDB; 37898at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0050454; F:coenzyme F420 hydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0052592; F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor; IEA:TreeGrafter.
DR   Gene3D; 3.10.450.750; -; 1.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR017679; FrhB_archaea.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   NCBIfam; TIGR03289; frhB; 1.
DR   NCBIfam; NF006807; PRK09325.1; 1.
DR   PANTHER; PTHR31332; 7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR31332:SF6; FORMATE DEHYDROGENASE SUBUNIT BETA; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000313|EMBL:ABC57682.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001931}.
FT   DOMAIN          14..90
FT                   /note="Coenzyme F420 hydrogenase/dehydrogenase beta subunit
FT                   N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF04422"
FT   DOMAIN          99..249
FT                   /note="Coenzyme F420 hydrogenase/dehydrogenase beta subunit
FT                   C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF04432"
SQ   SEQUENCE   298 AA;  32683 MW;  BFB513EE13A1A4EB CRC64;
     MIQDSVLGPH QEIITAKAAD NKVLSKAQDG GIVSAILIYA LEENIIDGTI VAGPGDEPWK
     PEPLVATTKK EILKGAGTKY TMCPNLSVIK EATREYGLEK IGTVGTPCQV MGLRKMQTYP
     LGVRNVVDKI ALSIGIYCME NFPYESLKTF INDKVGVTPS QVTKMNITKG KLFITHTGGE
     GKIPLKETHG YEQAGCNYCM DYVAELSDLS CGSVGAKPGW STIIKRTDKG ASLIDKAIKE
     GVLETTETNE GKFGLEMLRK LSTNKKKKHQ KRIDKQIDYG LNMPFTHSKD KNDPLENR
//