ID   FTHS_SYNAS              Reviewed;         565 AA.
AC   Q2LU82;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   18-JUN-2025, entry version 94.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=SYNAS_17640; ORFNames=SYN_02008;
OS   Syntrophus aciditrophicus (strain SB).
OC   Bacteria; Pseudomonadati; Thermodesulfobacteriota; Syntrophia;
OC   Syntrophales; Syntrophaceae; Syntrophus.
OX   NCBI_TaxID=56780;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SB;
RX   PubMed=17442750; DOI=10.1073/pnas.0610456104;
RA   McInerney M.J., Rohlin L., Mouttaki H., Kim U., Krupp R.S.,
RA   Rios-Hernandez L., Sieber J., Struchtemeyer C.G., Bhattacharyya A.,
RA   Campbell J.W., Gunsalus R.P.;
RT   "The genome of Syntrophus aciditrophicus: life at the thermodynamic limit
RT   of microbial growth.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7600-7605(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000252; ABC77643.1; -; Genomic_DNA.
DR   RefSeq; WP_011417665.1; NC_007759.1.
DR   AlphaFoldDB; Q2LU82; -.
DR   SMR; Q2LU82; -.
DR   STRING; 56780.SYN_02008; -.
DR   KEGG; sat:SYN_02008; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_7; -.
DR   InParanoid; Q2LU82; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001933; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniRule.
DR   CDD; cd00477; FTHFS; 1.
DR   FunFam; 3.30.1510.10:FF:000001; Formate--tetrahydrofolate ligase; 1.
DR   FunFam; 3.10.410.10:FF:000001; Putative formate--tetrahydrofolate ligase; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   NCBIfam; NF010030; PRK13505.1; 1.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..565
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293072"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   565 AA;  60940 MW;  B4CF77DEDE1BFDE1 CRC64;
     MTHRNSRQDT KELKPIADIA ATIGLSEDDI EAYGRYKAKV RLEAISRFHS RPDASLILVS
     AMTPTPAGEG KTTVSIGLAQ ALARLGKATI AALREPSLGP VFGMKGGATG GGLSRVHPVD
     DINLHFTNDF AAVESAHNLL SAIVDNSVYH DNILNIDPRK VTWRRVLDMN DRFLRNIVIG
     LGGSVNGVPR ETGFDIVPSS EIMAILCLSR SYRELKEKIR RILVGFTYDD SPVMAGDLKV
     EGAVTALLKY ALLPNLVQTT ENVPAIIHGG PFANIAQGTS SILGTDLALR LADYVVTEAG
     FGFDLGAEKF FDIVAPYGGL NPRIVVLVAT VRALKYHAGI AQADLDRSNP RAAVLGMANL
     RKHYQNIDKF HVSCVIALNR FSSDTDEEIN AVVRAAENEG MNIAPCDIFR LGGEGGLELA
     EKTLELLAGT SCGYRRLYEW NQPVEDKIFT VASEIYGAVS IDYQPLARRN LDLINKYGFD
     KLPVCIAKTQ QSLSDNPGLL GLPRDFIVTV REIRIASGAG FLIPITGEIL RMPGLSKRPA
     AYSIDIDDSG NITGVGSPGG ISSLS
//