ID   ACSF_RHOP2              Reviewed;         365 AA.
AC   Q2ISZ6;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   04-NOV-2026, entry version 99.
DE   RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase {ECO:0000255|HAMAP-Rule:MF_01840};
DE            EC=1.14.13.81 {ECO:0000255|HAMAP-Rule:MF_01840};
GN   Name=acsF {ECO:0000255|HAMAP-Rule:MF_01840};
GN   OrderedLocusNames=RPB_3971;
OS   Rhodopseudomonas palustris (strain HaA2).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Nitrobacteraceae; Rhodopseudomonas.
OX   NCBI_TaxID=316058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HaA2;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M.,
RA   Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A.,
RA   Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P.;
RT   "Complete sequence of Rhodopseudomonas palustris HaA2.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll
CC       biosynthesis. Mediates the cyclase reaction, which results in the
CC       formation of divinylprotochlorophyllide (Pchlide) characteristic of all
CC       chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester
CC       (MgPMME). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Mg-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 O2 + 2
CC         H(+) = 3,8-divinyl protochlorophyllide a + 3 NADP(+) + 5 H2O;
CC         Xref=Rhea:RHEA:33235, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:60491; EC=1.14.13.81;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01840};
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01840};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; bacteriochlorophyll
CC       biosynthesis (light-independent). {ECO:0000255|HAMAP-Rule:MF_01840}.
CC   -!- SIMILARITY: Belongs to the AcsF family. {ECO:0000255|HAMAP-
CC       Rule:MF_01840}.
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DR   EMBL; CP000250; ABD08664.1; -; Genomic_DNA.
DR   RefSeq; WP_011442848.1; NC_007778.1.
DR   AlphaFoldDB; Q2ISZ6; -.
DR   SMR; Q2ISZ6; -.
DR   STRING; 316058.RPB_3971; -.
DR   KEGG; rpb:RPB_3971; -.
DR   eggNOG; COG1633; Bacteria.
DR   HOGENOM; CLU_048037_0_0_5; -.
DR   OMA; AAMHHHR; -.
DR   OrthoDB; 141643at2; -.
DR   UniPathway; UPA00671; -.
DR   Proteomes; UP000008809; Chromosome.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0036070; P:light-independent bacteriochlorophyll biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd01047; ACSF; 1.
DR   HAMAP; MF_01840; AcsF; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR009078; Ferritin-like_SF.
DR   InterPro; IPR003251; Rr_diiron-bd_dom.
DR   NCBIfam; TIGR02029; AcsF; 1.
DR   NCBIfam; NF010172; PRK13654.1; 1.
DR   PANTHER; PTHR31053; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR31053:SF2; MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER [OXIDATIVE] CYCLASE, CHLOROPLASTIC; 1.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   SUPFAM; SSF47240; Ferritin-like; 1.
PE   3: Inferred from homology;
KW   Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Iron;
KW   Metal-binding; NADP; Oxidoreductase; Photosynthesis; Reference proteome.
FT   CHAIN           1..365
FT                   /note="Aerobic magnesium-protoporphyrin IX monomethyl ester
FT                   [oxidative] cyclase"
FT                   /id="PRO_1000070550"
SQ   SEQUENCE   365 AA;  42415 MW;  09DC0949FD6B91F5 CRC64;
     MIPMEGGAQS ALRSRPAIKG SVESLNIAKE DTILTPRFYT TDYAAMDKLD VSLVRSEWNV
     MMNEMRADYN KSHFKKNDEF LESDLDKLPP ALRAEFKDFL VSSLTAEFSG CVLYAEIKKR
     IKNPEIRELF GLLSRDEARH AGFINEILKD HGIGVDLSFL TKVKKYTYFR PKFIFYATYL
     SEKIGYARYI TIYRQMERHP ERRFHPIFKW FERWCNDEFR HGEAFALLMR ADPSLLSGVN
     KLWIRFFLLA VFATMYVRDH MRPAFYEALG MDAAEYGMQV FRITTEISKQ VFPVTINLDD
     PRFLAGLERL RVASEKLADC RSQGFVGKLK RPFYVASAAL AFGRLFLLPA KRNELPRVIG
     LRPAW
//