ID   Q2FT06_METHJ            Unreviewed;       175 AA.
AC   Q2FT06;
DT   21-MAR-2006, integrated into UniProtKB/TrEMBL.
DT   21-MAR-2006, sequence version 1.
DT   10-JUN-2026, entry version 84.
DE   RecName: Full=pyruvate synthase {ECO:0000256|ARBA:ARBA00012822};
DE            EC=1.2.7.1 {ECO:0000256|ARBA:ARBA00012822};
GN   OrderedLocusNames=Mhun_2394 {ECO:0000313|EMBL:ABD42096.1};
OS   Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS   JF-1).
OC   Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC   Methanomicrobia; Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX   NCBI_TaxID=323259 {ECO:0000313|EMBL:ABD42096.1, ECO:0000313|Proteomes:UP000001941};
RN   [1] {ECO:0000313|Proteomes:UP000001941}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1
RC   {ECO:0000313|Proteomes:UP000001941};
RX   PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA   Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA   Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA   Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT   "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL   Stand. Genomic Sci. 11:2-2(2016).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced
CC         [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+);
CC         Xref=Rhea:RHEA:12765, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15361, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=1.2.7.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00049357};
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DR   EMBL; CP000254; ABD42096.1; -; Genomic_DNA.
DR   RefSeq; WP_011449354.1; NC_007796.1.
DR   AlphaFoldDB; Q2FT06; -.
DR   STRING; 323259.Mhun_2394; -.
DR   EnsemblBacteria; ABD42096; ABD42096; Mhun_2394.
DR   GeneID; 3923292; -.
DR   KEGG; mhu:Mhun_2394; -.
DR   eggNOG; arCOG01603; Archaea.
DR   HOGENOM; CLU_087284_2_0_2; -.
DR   InParanoid; Q2FT06; -.
DR   OrthoDB; 372091at2157; -.
DR   Proteomes; UP000001941; Chromosome.
DR   GO; GO:0019164; F:pyruvate synthase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.920.10; Pyruvate-ferredoxin oxidoreductase, PFOR, domain III; 1.
DR   InterPro; IPR051626; Oxidoreductase_gamma_subunit.
DR   InterPro; IPR011894; PorC_KorC.
DR   InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat.
DR   InterPro; IPR002869; Pyrv_flavodox_OxRed_cen.
DR   NCBIfam; TIGR02175; PorC_KorC; 1.
DR   PANTHER; PTHR43366; PYRUVATE SYNTHASE SUBUNIT PORC; 1.
DR   PANTHER; PTHR43366:SF1; PYRUVATE SYNTHASE SUBUNIT PORC; 1.
DR   Pfam; PF01558; POR; 1.
DR   SUPFAM; SSF53323; Pyruvate-ferredoxin oxidoreductase, PFOR, domain III; 1.
PE   4: Predicted;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000313|EMBL:ABD42096.1}; Pyruvate {ECO:0000313|EMBL:ABD42096.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001941}.
FT   DOMAIN          3..172
FT                   /note="Pyruvate/ketoisovalerate oxidoreductase catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01558"
SQ   SEQUENCE   175 AA;  18915 MW;  23029E1EC6E1A68E CRC64;
     MYEIRLHSRG GQGGVTAAKL MALASFRDGK FATAAPFYGA ERRGAPIVSF IRIDDKPIKV
     YSQIHNPDLI VVLDPSILDL VNVFEGLKPD GKVLINAPDI PKKAEGYQVR YVDLTGIALK
     TNLVVAGSPI LNTPVIGALA KMGLFSRESA AAAIKEMFSD ERNLKAALMA YEELV
//