Q0T9X8 NRFA_ECOL5 Cytochrome c-552 1.7.2.2 Ammonia-forming cytochrome c nitrite reductase Cytochrome c nitrite reductase nrfA ECP_4303 Escherichia coli O6:K15:H31 (strain 536 / UPEC) Bacteria Pseudomonadati Pseudomonadota Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] 536 / UPEC Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process. 6 Fe(III)-[cytochrome c] + NH4(+) + 2 H2O = 6 Fe(II)-[cytochrome c] + nitrite + 8 H(+) Ca(2+) Binds 1 Ca(2+) ion per monomer. heme c Binds 5 heme c groups covalently per monomer. Nitrogen metabolism; nitrate reduction (assimilation). Periplasm Belongs to the cytochrome c-552 family. Calcium Electron transport Heme Iron Metal-binding Oxidoreductase Periplasm Signal Transport heme c 3 Fe heme 1 heme 1 heme 1 Fe heme c 2 heme c 2 heme c 2 Fe heme c 3 heme c 3 heme c 3 Fe Ca(2+) Ca(2+) substrate Ca(2+) Ca(2+) substrate heme c 5 Fe heme c 4 heme c 4 heme c 4 Fe heme c 2 Fe heme c 5 heme c 5 heme c 5 Fe heme c 4 Fe MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNETFAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVRETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGCADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYFDGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHGKNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQSINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHMHAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQIKAEKQDFIKTVIPQWEEQARKNGLLSQ Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License