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<entry dataset="Swiss-Prot" created="2008-02-05" modified="2026-11-04" version="103" xmlns="http://uniprot.org/uniprot">
  <accession>Q0T9J5</accession>
  <name>ULAF_ECOL5</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">L-ribulose-5-phosphate 4-epimerase UlaF</fullName>
      <ecNumber evidence="1">5.1.3.4</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">L-ascorbate utilization protein F</fullName>
    </alternativeName>
    <alternativeName>
      <fullName evidence="1">Phosphoribulose isomerase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">ulaF</name>
    <name type="ordered locus">ECP_4443</name>
  </gene>
  <organism>
    <name type="scientific">Escherichia coli O6:K15:H31 (strain 536 / UPEC)</name>
    <dbReference type="NCBI Taxonomy" id="362663"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Gammaproteobacteria</taxon>
      <taxon>Enterobacterales</taxon>
      <taxon>Enterobacteriaceae</taxon>
      <taxon>Escherichia</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2006" name="Mol. Microbiol." volume="61" first="584" last="595">
      <title>Role of pathogenicity island-associated integrases in the genome plasticity of uropathogenic Escherichia coli strain 536.</title>
      <authorList>
        <person name="Hochhut B."/>
        <person name="Wilde C."/>
        <person name="Balling G."/>
        <person name="Middendorf B."/>
        <person name="Dobrindt U."/>
        <person name="Brzuszkiewicz E."/>
        <person name="Gottschalk G."/>
        <person name="Carniel E."/>
        <person name="Hacker J."/>
      </authorList>
      <dbReference type="PubMed" id="16879640"/>
      <dbReference type="DOI" id="10.1111/j.1365-2958.2006.05255.x"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>536 / UPEC</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>L-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
      <dbReference type="Rhea" id="RHEA:22368"/>
      <dbReference type="ChEBI" id="CHEBI:57737"/>
      <dbReference type="ChEBI" id="CHEBI:58226"/>
      <dbReference type="EC" id="5.1.3.4"/>
    </reaction>
  </comment>
  <comment type="cofactor">
    <cofactor evidence="1">
      <name>Zn(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:29105"/>
    </cofactor>
    <text evidence="1">Binds 1 zinc ion per subunit.</text>
  </comment>
  <comment type="pathway">
    <text evidence="1">Cofactor degradation; L-ascorbate degradation; D-xylulose 5-phosphate from L-ascorbate: step 4/4.</text>
  </comment>
  <comment type="induction">
    <text evidence="1">Induced by L-ascorbate. Repressed by UlaR.</text>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the aldolase class II family. AraD/FucA subfamily.</text>
  </comment>
  <dbReference type="EC" id="5.1.3.4" evidence="1"/>
  <dbReference type="EMBL" id="CP000247">
    <property type="protein sequence ID" value="ABG72384.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_001170781.1">
    <property type="nucleotide sequence ID" value="NC_008253.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="Q0T9J5"/>
  <dbReference type="SMR" id="Q0T9J5"/>
  <dbReference type="KEGG" id="ecp:ECP_4443"/>
  <dbReference type="HOGENOM" id="CLU_006033_5_0_6"/>
  <dbReference type="OMA" id="ADHAVHN"/>
  <dbReference type="UniPathway" id="UPA00263">
    <property type="reaction ID" value="UER00380"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005829">
    <property type="term" value="C:cytosol"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0016832">
    <property type="term" value="F:aldehyde-lyase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="GO" id="GO:0008742">
    <property type="term" value="F:L-ribulose-phosphate 4-epimerase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0008270">
    <property type="term" value="F:zinc ion binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0019854">
    <property type="term" value="P:L-ascorbic acid catabolic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0019323">
    <property type="term" value="P:pentose catabolic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="TreeGrafter"/>
  </dbReference>
  <dbReference type="CDD" id="cd00398">
    <property type="entry name" value="Aldolase_II"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="FunFam" id="3.40.225.10:FF:000001">
    <property type="entry name" value="L-ribulose-5-phosphate 4-epimerase UlaF"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.40.225.10">
    <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_01952">
    <property type="entry name" value="UlaF"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR050197">
    <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR001303">
    <property type="entry name" value="Aldolase_II/adducin_N"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR036409">
    <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR023499">
    <property type="entry name" value="UlaF"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF006047">
    <property type="entry name" value="araD"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF009003">
    <property type="entry name" value="PRK12348.1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR22789">
    <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR22789:SF9">
    <property type="entry name" value="L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE ULAF"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF00596">
    <property type="entry name" value="Aldolase_II"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SMART" id="SM01007">
    <property type="entry name" value="Aldolase_II"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF53639">
    <property type="entry name" value="AraD/HMP-PK domain-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0119">Carbohydrate metabolism</keyword>
  <keyword id="KW-0413">Isomerase</keyword>
  <keyword id="KW-0479">Metal-binding</keyword>
  <keyword id="KW-0862">Zinc</keyword>
  <feature type="chain" id="PRO_1000070639" description="L-ribulose-5-phosphate 4-epimerase UlaF">
    <location>
      <begin position="1"/>
      <end position="228"/>
    </location>
  </feature>
  <feature type="active site" description="Proton donor/acceptor" evidence="1">
    <location>
      <position position="118"/>
    </location>
  </feature>
  <feature type="active site" description="Proton donor/acceptor" evidence="1">
    <location>
      <position position="225"/>
    </location>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="26"/>
      <end position="27"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="43"/>
      <end position="44"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="72"/>
      <end position="73"/>
    </location>
    <ligand>
      <name>substrate</name>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="74"/>
    </location>
    <ligand>
      <name>Zn(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:29105"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="93"/>
    </location>
    <ligand>
      <name>Zn(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:29105"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="95"/>
    </location>
    <ligand>
      <name>Zn(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:29105"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="167"/>
    </location>
    <ligand>
      <name>Zn(2+)</name>
      <dbReference type="ChEBI" id="CHEBI:29105"/>
    </ligand>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_01952"/>
    </source>
  </evidence>
  <sequence length="228" mass="25306" checksum="C2C942553FF3B464" modified="2006-09-05" version="1">MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSGVAYETMKADDMVVVDMSGNVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAWAQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTPGIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRKHGPNAYYGQK</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>