##gff-version 3 Q09639 UniProtKB Chain 1 707 . . . ID=PRO_0000086841;Note=G protein-coupled receptor kinase 2 Q09639 UniProtKB Domain 54 175 . . . Note=RGS;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00171 Q09639 UniProtKB Domain 191 455 . . . Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 Q09639 UniProtKB Domain 456 523 . . . Note=AGC-kinase C-terminal;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00618 Q09639 UniProtKB Domain 558 658 . . . Note=PH;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00145 Q09639 UniProtKB Region 1 190 . . . Note=N-terminal Q09639 UniProtKB Active site 318 318 . . . Note=Proton acceptor;Ontology_term=ECO:0000255,ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159,ECO:0000255|PROSITE-ProRule:PRU10027 Q09639 UniProtKB Binding site 197 205 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 Q09639 UniProtKB Binding site 220 220 . . . Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 Q09639 UniProtKB Site 3 3 . . . Note=Required for receptor phosphorylation;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P25098 Q09639 UniProtKB Site 4 4 . . . Note=Required for receptor phosphorylation;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P25098 Q09639 UniProtKB Site 10 10 . . . Note=Required for receptor phosphorylation;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P25098 Q09639 UniProtKB Mutagenesis 3 3 . . . Note=Failure to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. D->K;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 4 4 . . . Note=Failure to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. L->K;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 7 8 . . . Note=Failure to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. VL->AA;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 10 10 . . . Note=Failure to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. D->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 106 106 . . . Note=No effect on ability to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. R->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 109 109 . . . Note=No effect on ability to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. Y->I;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 110 110 . . . Note=No effect on ability to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. D->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 195 195 . . . Note=Failure to restore locomotion or avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. R->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 220 220 . . . Note=Failure to restore avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. Failure to rescue defective egg laying and locomotion in grk-2 mutants. K->R;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28213524,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28213524,PMID:28968387 Q09639 UniProtKB Mutagenesis 354 354 . . . Note=In rt97%3B dramatic decrease in grk-2 protein levels%2C defective egg laying%2C reduced serotonin levels%2C increased levels of the serotonin metabolite 5-hydroxyindoleacetic acid%2C increased levels of amine oxidase amx-2 in early adult stages%2C reduced levels of phosphorylated amx-2%2C severe defect in octanol avoidance and severely impaired chemotaxis to attractive compounds including 0.1-100 mMNaCl although mutants show avoidance to 25 mMNaCl after pre-exposure to 100 mMNaCl. T->I;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15157420,ECO:0000269|PubMed:16407969,ECO:0000269|PubMed:28213524;Dbxref=PMID:15157420,PMID:16407969,PMID:28213524 Q09639 UniProtKB Mutagenesis 379 379 . . . Note=In yak18%3B decreased body length%2C slow locomotion and defective egg laying. G->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:28968387;Dbxref=PMID:28968387 Q09639 UniProtKB Mutagenesis 567 567 . . . Note=Failure to restore locomotion in animals lacking endogenous grk-2. Significantly restored avoidance of aversive compounds octanol and quinine in animals lacking endogenous grk-2. Failure to restore avoidance to octanol and quinine in animals lacking endogenous grk-2%3B when associated with Q-587. K->E;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387 Q09639 UniProtKB Mutagenesis 587 587 . . . Note=Failure to restore locomotion in animals lacking endogenous grk-2. Reduced ability to restore avoidance to the aversive compounds octanol and quinine in animals lacking endogenous grk-2. Failure to restore avoidance to octanol and quinine in animals lacking endogenous grk-2%3B when associated with E-567. R->Q;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22375004,ECO:0000269|PubMed:28968387;Dbxref=PMID:22375004,PMID:28968387