ID   RPAL2_PELHO             Reviewed;         322 AA.
AC   Q06FN4;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   02-SEP-2026, entry version 71.
DE   RecName: Full=Putative DNA-directed RNA polymerase subunit alpha-like 2;
DE            Short=Putative PEP 2;
DE            EC=2.7.7.6;
DE   AltName: Full=Putative plastid-encoded RNA polymerase subunit alpha 2;
DE            Short=Putative RNA polymerase subunit alpha 2;
GN   Name=rpoAL2-A; Synonyms=ORF332;
GN   and
GN   Name=rpoAL2-B; Synonyms=ORF332;
OS   Pelargonium hortorum (Common geranium) (Pelargonium inquinans x Pelargonium
OS   zonale).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Geraniales; Geraniaceae; Pelargonium.
OX   NCBI_TaxID=4031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Ringo White;
RX   PubMed=16916942; DOI=10.1093/molbev/msl089;
RA   Chumley T.W., Palmer J.D., Mower J.P., Fourcade H.M., Calie P.J.,
RA   Boore J.L., Jansen R.K.;
RT   "The complete chloroplast genome sequence of Pelargonium x hortorum:
RT   organization and evolution of the largest and most highly rearranged
RT   chloroplast genome of land plants.";
RL   Mol. Biol. Evol. 23:2175-2190(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) +
CC         diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC       transcription, whereas the C-terminal domain is involved in interaction
CC       with transcriptional regulators and with upstream promoter elements.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. There are 3 rpoA-like
CC       genes in this organism (found in the inverted repeat). None of them are
CC       convincing as rpoA, and it may be that the functional gene is in the
CC       nucleus. {ECO:0000305}.
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DR   EMBL; DQ897681; ABI17301.1; -; Genomic_DNA.
DR   EMBL; DQ897681; ABI17339.1; -; Genomic_DNA.
DR   RefSeq; YP_784109.1; NC_008454.1.
DR   RefSeq; YP_784147.1; NC_008454.1.
DR   AlphaFoldDB; Q06FN4; -.
DR   SMR; Q06FN4; -.
DR   GeneID; 4362844; -.
DR   GeneID; 4362953; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR   Gene3D; 2.170.120.12; DNA-directed RNA polymerase, insert domain; 1.
DR   Gene3D; 3.30.1360.10; RNA polymerase, RBP11-like subunit; 1.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   SUPFAM; SSF56553; Insert subdomain of RNA polymerase alpha subunit; 1.
DR   SUPFAM; SSF55257; RBP11-like subunits of RNA polymerase; 1.
PE   5: Uncertain;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..322
FT                   /note="Putative DNA-directed RNA polymerase subunit alpha-
FT                   like 2"
FT                   /id="PRO_0000296902"
FT   REGION          1..232
FT                   /note="Alpha N-terminal domain (alpha-NTD)"
FT                   /evidence="ECO:0000250"
FT   REGION          280..322
FT                   /note="Alpha C-terminal domain (alpha-CTD)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   322 AA;  36122 MW;  C0DB7394BBA2A4D9 CRC64;
     MSNPNNGAEW QQVEADQLDS GLYYGRFALS PLTAKQASLL KKGLPEALLT EILCLRFTHA
     KIQNECVNLM NIVGIQESLD EILKNFGKII LTGKLEEFVG KGPFVAILDV RGPLNAMAVD
     IELPPGIKVE IETQHIATIT EPIPFVVELK IELVSSTSKG ETGITDEEGF SIDPNPPIQK
     VDTSIQCYDY QGEPFQTLFL DIWTDRTIHP HEALAQASRK IFGLLSLVFQ AEYFQYNELE
     NGLRYGKFCL YPMTKEQFQW IQTALNEALA LDMSGESKHE GPVTDEEGDS IDPTFTPVQK
     WDITMNSYQY SGETFQGLLS RF
//