ID   GUN1_SAIFL              Reviewed;         341 AA.
AC   Q04469;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-SEP-2026, entry version 79.
DE   RecName: Full=Endoglucanase 1;
DE            EC=3.2.1.4;
DE   AltName: Full=Carboxymethyl-cellulase 1;
DE            Short=CMCase 1;
DE            Short=Cellulase 1;
DE   AltName: Full=Endo-1,4-beta-glucanase 1;
DE   Flags: Precursor;
GN   Name=CMC1;
OS   Saitozyma flava (Cryptococcus flavus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Trimorphomycetaceae; Saitozyma.
OX   NCBI_TaxID=5416;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=1368837; DOI=10.1271/bbb.56.1230;
RA   Cui Z., Mochizuki D., Matsuno Y., Nakamura T., Liu Y., Hatano T., Fukui S.,
RA   Miyakawa T.;
RT   "Cloning and molecular analysis of cDNA encoding a carboxymethylcellulase
RT   of the yeast Cryptococcus flavus.";
RL   Biosci. Biotechnol. Biochem. 56:1230-1235(1992).
CC   -!- FUNCTION: Has endoglucanase activity on carboxymethyl-cellulose (CMC).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
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DR   EMBL; D13967; BAA03070.1; -; mRNA.
DR   EMBL; S45137; AAC60541.1; -; Genomic_DNA.
DR   PIR; JC1201; JC1201.
DR   AlphaFoldDB; Q04469; -.
DR   SMR; Q04469; -.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009251; P:glucan catabolic process; IEA:TreeGrafter.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   PANTHER; PTHR34142:SF5; CBM1 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation;
KW   Cleavage on pair of basic residues; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Signal.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..30
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000007855"
FT   CHAIN           31..341
FT                   /note="Endoglucanase 1"
FT                   /id="PRO_0000007856"
FT   ACT_SITE        166
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        275
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   341 AA;  35717 MW;  4A62E45C952DD120 CRC64;
     MKTATLLAAL SVLAGALAAP LAGDSALHRR SLPRLGGVNL AGCDFGIDIY GNSGTPACPG
     TEQVGHFIAD GANLFRLPAG WQYLVGNNQA STSLAPDFFA QYDALVQAVI SKGAYAIIDV
     HNYARWNGAI IGQGGPSNQD FANLWTLLAT KVTSNDPNVI FGLMNEPHDL DVSTWAGSVQ
     AAVNAIRAAG ATSQYILIPG TGFTNANAWF QGQDNALLGV TDPVGGTDKL LLDVHRYNDV
     DFSGTHAECT TNSLDVLSSL DSWLKGNGRK AIVSETGGGH TTSCETDLGE FLNGIKEDYP
     SVLGFAVWAA GSFDPSYVLS ITPTNGVDNQ LFDIAVKPNL P
//