ID   AGE1_YEAST              Reviewed;         482 AA.
AC   Q04412; D6VTE4;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   02-SEP-2026, entry version 167.
DE   RecName: Full=ADP-ribosylation factor GTPase-activating protein effector protein 1;
DE            Short=ARF GAP effector protein 1;
DE   AltName: Full=Suppressor of ARF1 thermosensitive mutant protein 1;
GN   Name=AGE1; Synonyms=SAT1; OrderedLocusNames=YDR524C;
GN   ORFNames=D9719.28;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867; DOI=10.1038/387s075;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION.
RX   PubMed=9677411; DOI=10.1074/jbc.273.31.19792;
RA   Zhang C.-J., Cavenagh M.M., Kahn R.A.;
RT   "A family of Arf effectors defined as suppressors of the loss of Arf
RT   function in the yeast Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 273:19792-19796(1998).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   FUNCTION.
RX   PubMed=12627398; DOI=10.1002/yea.966;
RA   Zhang C.-J., Bowzard J.B., Anido A., Kahn R.A.;
RT   "Four ARF GAPs in Saccharomyces cerevisiae have both overlapping and
RT   distinct functions.";
RL   Yeast 20:315-330(2003).
CC   -!- FUNCTION: GTPase-activating protein (GAP) for the ADP ribosylation
CC       factors ARF1 and ARF2. May be involved in the endocytic pathway.
CC       {ECO:0000269|PubMed:12627398, ECO:0000269|PubMed:9677411}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U33057; AAB64964.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12354.1; -; Genomic_DNA.
DR   PIR; S69580; S69580.
DR   RefSeq; NP_010812.3; NM_001180832.3.
DR   AlphaFoldDB; Q04412; -.
DR   SMR; Q04412; -.
DR   BioGRID; 32574; 89.
DR   DIP; DIP-5273N; -.
DR   FunCoup; Q04412; 154.
DR   IntAct; Q04412; 3.
DR   MINT; Q04412; -.
DR   STRING; 4932.YDR524C; -.
DR   iPTMnet; Q04412; -.
DR   PaxDb; 4932-YDR524C; -.
DR   PeptideAtlas; Q04412; -.
DR   EnsemblFungi; YDR524C_mRNA; YDR524C; YDR524C.
DR   GeneID; 852136; -.
DR   KEGG; sce:YDR524C; -.
DR   AGR; SGD:S000002932; -.
DR   SGD; S000002932; AGE1.
DR   VEuPathDB; FungiDB:YDR524C; -.
DR   eggNOG; KOG0521; Eukaryota.
DR   HOGENOM; CLU_493595_0_0_1; -.
DR   InParanoid; Q04412; -.
DR   OMA; THSHHIN; -.
DR   OrthoDB; 10266696at2759; -.
DR   BioCyc; YEAST:G3O-30040-MONOMER; -.
DR   BioGRID-ORCS; 852136; 2 hits in 10 CRISPR screens.
DR   PRO; PR:Q04412; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q04412; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005768; C:endosome; IDA:SGD.
DR   GO; GO:0010008; C:endosome membrane; IBA:GO_Central.
DR   GO; GO:0005802; C:trans-Golgi network; IDA:SGD.
DR   GO; GO:0005096; F:GTPase activator activity; IDA:SGD.
DR   GO; GO:0005543; F:phospholipid binding; IDA:SGD.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IGI:SGD.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IGI:SGD.
DR   CDD; cd08204; ArfGap; 1.
DR   FunFam; 1.10.220.150:FF:000032; ARF GAP; 1.
DR   Gene3D; 1.10.220.150; Arf GTPase activating protein; 1.
DR   InterPro; IPR045258; ACAP1/2/3-like.
DR   InterPro; IPR037278; ARFGAP/RecO.
DR   InterPro; IPR001164; ArfGAP_dom.
DR   InterPro; IPR038508; ArfGAP_dom_sf.
DR   PANTHER; PTHR23180:SF160; ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1; 1.
DR   PANTHER; PTHR23180; CENTAURIN/ARF; 1.
DR   Pfam; PF01412; ArfGap; 1.
DR   SMART; SM00105; ArfGap; 1.
DR   SUPFAM; SSF57863; ArfGap/RecO-like zinc finger; 1.
DR   PROSITE; PS50115; ARFGAP; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; GTPase activation; Metal-binding; Protein transport;
KW   Reference proteome; Transport; Zinc; Zinc-finger.
FT   CHAIN           1..482
FT                   /note="ADP-ribosylation factor GTPase-activating protein
FT                   effector protein 1"
FT                   /id="PRO_0000074222"
FT   DOMAIN          170..297
FT                   /note="Arf-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT   ZN_FING         186..210
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00288"
FT   REGION          116..156
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        118..127
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..143
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   482 AA;  54494 MW;  3F7682F91EA21EDD CRC64;
     MDFYTTDINK NVVPLFSKGT VARTASKAQY PSWCNNALKL TNILLKSLRC KFQTNRCEDD
     RGFEVYCVIL KSIALLMAAK ESLILLQIPP SLPSGFPFRS PQLSFTYLST RLSGSQHKST
     HSHHINHQTH PIHSSSSNSN SNNRIPTKTD SSKQHTQHFS FANAGASNRD ELLSIVRKID
     KSNLKCCDCG STATVEWVSI NLLCILCIKC SGVHRSLGSH ISKIRSLTLD NFTSLELMHL
     LQNNVSNSNV NAIYESNLRN FPVKKITANS DDSERSKFII DKYQFKKFVI DSNQGREASL
     KSLIKAIHLD SVFMMQRAIA QSKYSLRELT ASEKEQNDLN HSSIFQYSLK HYEIVDGTPI
     FFITEFLLCN GIHIDNLPKI TTNWSPKVLE YWETKLEMYG TFQAVNTSRP RSGPHLNMHS
     NVDSASSYNK KHDLRVNIPE RSASASKRWS LSSIPKSSQN LMSPTNLLTM HKSLKLAKKD
     KK
//