ID   Q03XT2_LEUMM            Unreviewed;       740 AA.
AC   Q03XT2;
DT   14-NOV-2006, integrated into UniProtKB/TrEMBL.
DT   14-NOV-2006, sequence version 1.
DT   04-NOV-2026, entry version 104.
DE   RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE            EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN   OrderedLocusNames=LEUM_0882 {ECO:0000313|EMBL:ABJ61990.1};
OS   Leuconostoc mesenteroides subsp. mesenteroides (strain ATCC 8293 / DSM
OS   20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO 523 / NBRC 100496 / NCIMB
OS   8023 / NCTC 12954 / NRRL B-1118 / 37Y).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=203120 {ECO:0000313|EMBL:ABJ61990.1, ECO:0000313|Proteomes:UP000000362};
RN   [1] {ECO:0000313|EMBL:ABJ61990.1, ECO:0000313|Proteomes:UP000000362}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8293 / DSM 20343 / BCRC 11652 / CCM 1803 / JCM 6124 / NCDO
RC   523 / NBRC 100496 / NCIMB 8023 / NCTC 12954 / NRRL B-1118 / 37Y
RC   {ECO:0000313|Proteomes:UP000000362};
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K., Slesarev A., Wolf Y., Sorokin A., Mirkin B., Koonin E.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M., Klaenhammer T., Richardson P.,
RA   Kozyavkin S., Weimer B., Mills D.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC         Evidence={ECO:0000256|ARBA:ARBA00001255,
CC         ECO:0000256|PIRNR:PIRNR005536};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC       {ECO:0000256|ARBA:ARBA00006202}.
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DR   EMBL; CP000414; ABJ61990.1; -; Genomic_DNA.
DR   RefSeq; WP_011679647.1; NC_008531.1.
DR   EnsemblBacteria; ABJ61990; ABJ61990; LEUM_0882.
DR   GeneID; 29575810; -.
DR   KEGG; lme:LEUM_0882; -.
DR   eggNOG; COG3345; Bacteria.
DR   HOGENOM; CLU_009640_2_1_9; -.
DR   OMA; AAHNQTQ; -.
DR   CDD; cd14791; GH36; 1.
DR   FunFam; 3.20.20.70:FF:000118; Alpha-galactosidase; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR038417; Alpga-gal_N_sf.
DR   InterPro; IPR050985; Alpha-glycosidase_related.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR   InterPro; IPR002252; Glyco_hydro_36.
DR   InterPro; IPR031705; Glyco_hydro_36_C.
DR   InterPro; IPR031704; Glyco_hydro_36_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR   PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR   Pfam; PF16874; Glyco_hydro_36C; 1.
DR   Pfam; PF16875; Glyco_hydro_36N; 1.
DR   Pfam; PF02065; Melibiase; 1.
DR   PIRSF; PIRSF005536; Agal; 1.
DR   PRINTS; PR00743; GLHYDRLASE36.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
DR   GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR   Proteomes; UP000000362; Chromosome.
DR   AlphaFoldDB; Q03XT2; -.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000362}.
FT   DOMAIN          32..292
FT                   /note="Glycosyl hydrolase family 36 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16875"
FT   DOMAIN          657..734
FT                   /note="Glycosyl hydrolase family 36 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16874"
FT   ACT_SITE        487
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT   ACT_SITE        557
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ   SEQUENCE   740 AA;  84371 MW;  B4B77F090EEF3995 CRC64;
     MANANISFDD KQNVFHLSNR QISYLIQIEE GGLLSHLYFG KKITKYHGSR HYPRLDRGFS
     PNLAGKPHQT DRSYSKDTLL QEYGGYNTGD FRQAAIKIQG ANGAQTSDFR FQSFEIVNGK
     PHIAHLPQAY VLSEDEAKTL IIYLVDSALN VQVSLYYTIY NDRPVVARST KITNLSQQSI
     NIEKIASLQL DLPASDLEII SLPGAHVRER QMERQTVRQG VAEFSSHRGT SSHHMNPFVA
     LVTPNTTENE GEAVGIQLVY SGNHQFTIER DYVEQTRVLS GINEDGFDWQ LNADDDFQTP
     EALLVYSDEG LNGMSQAYHH LLRERVARGK YQYAERPIVI NNWEATYFDF NAEKIQEILD
     EASPLGIEMF VLDDGWFGKR DDDDSSLGDW FEYATKLQSG GGLVGLSDKV HEKGMKFGLW
     FEPEMISKNS DLYRQHPDYI LGMPNRDLTP SRDQYVLDFS RPEVVDNIFQ QVVNILDNVK
     IDYIKWDMNR HLTEVYSAAL SPERQGEVGH RYMLGVYDLA DRLTKRYPDI LFEGCSGGGG
     RFDAGMLYFF PQSWTSDNTD PVERLKIQYG TTLSYPVSSM TAHVSASPNH QTGRQTSLAM
     RGDVATAGVF GYELNLAKLS LEEKSEIKDQ VTFYKQHRSL IQYGNFYRIK SPFKNNETAW
     EFISEDKTEA LLFNYRVLST AQPVFTVTKM SHLKSDGIYV DQRTNEHYSG DELMSIGLYN
     TPIKQADFTS AVRYFKWQAE
//