ID   Q03GR7_PEDPA            Unreviewed;       733 AA.
AC   Q03GR7;
DT   14-NOV-2006, integrated into UniProtKB/TrEMBL.
DT   14-NOV-2006, sequence version 1.
DT   02-SEP-2026, entry version 93.
DE   RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE            EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN   OrderedLocusNames=PEPE_0513 {ECO:0000313|EMBL:ABJ67605.1};
OS   Pediococcus pentosaceus (strain ATCC 25745 / CCUG 21536 / LMG 10740 /
OS   183-1w).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Pediococcus.
OX   NCBI_TaxID=278197 {ECO:0000313|EMBL:ABJ67605.1, ECO:0000313|Proteomes:UP000000773};
RN   [1] {ECO:0000313|EMBL:ABJ67605.1, ECO:0000313|Proteomes:UP000000773}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25745 / CCUG 21536 / LMG 10740 / 183-1w
RC   {ECO:0000313|Proteomes:UP000000773};
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K., Slesarev A., Wolf Y., Sorokin A., Mirkin B., Koonin E.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M., Klaenhammer T., Richardson P.,
RA   Kozyavkin S., Weimer B., Mills D.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC         residues in alpha-D-galactosides, including galactose
CC         oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC         Evidence={ECO:0000256|ARBA:ARBA00001255,
CC         ECO:0000256|PIRNR:PIRNR005536};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC       {ECO:0000256|ARBA:ARBA00006202}.
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DR   EMBL; CP000422; ABJ67605.1; -; Genomic_DNA.
DR   RefSeq; WP_011673108.1; NC_008525.1.
DR   AlphaFoldDB; Q03GR7; -.
DR   STRING; 278197.PEPE_0513; -.
DR   GeneID; 33061910; -.
DR   KEGG; ppe:PEPE_0513; -.
DR   eggNOG; COG3345; Bacteria.
DR   HOGENOM; CLU_009640_2_1_9; -.
DR   OrthoDB; 9758822at2; -.
DR   Proteomes; UP000000773; Chromosome.
DR   GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR   CDD; cd14791; GH36; 1.
DR   FunFam; 3.20.20.70:FF:000118; Alpha-galactosidase; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR038417; Alpga-gal_N_sf.
DR   InterPro; IPR050985; Alpha-glycosidase_related.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR   InterPro; IPR002252; Glyco_hydro_36.
DR   InterPro; IPR031705; Glyco_hydro_36_C.
DR   InterPro; IPR031704; Glyco_hydro_36_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR   PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR   Pfam; PF16874; Glyco_hydro_36C; 1.
DR   Pfam; PF16875; Glyco_hydro_36N; 1.
DR   Pfam; PF02065; Melibiase; 1.
DR   PIRSF; PIRSF005536; Agal; 1.
DR   PRINTS; PR00743; GLHYDRLASE36.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000773}.
FT   DOMAIN          30..287
FT                   /note="Glycosyl hydrolase family 36 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16875"
FT   DOMAIN          650..727
FT                   /note="Glycosyl hydrolase family 36 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16874"
FT   ACT_SITE        480
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT   ACT_SITE        550
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ   SEQUENCE   733 AA;  83311 MW;  DC08B4509E413907 CRC64;
     MSLITVDQAN RVFHLHNQNL SYIFAVEQGG TLSHLYFGGH VDHYHGELRY PRVDRGFSGN
     LPGSTDRTFS RDTLPKEYST AGEMDYHLPA AIVRHTDGAN ALFLVYQGYR IEAGKPKLSG
     LPAAFVEDET EAETLTIVLV DQVSQVEFDL QYTIYRDRPV VTRSVQVCNQ GDHAVNLEKV
     ASMQIDFTDR QFETITLPGA HANERHPERG SINYGIQTFG SLRGTSSHQM NPFLALVDHT
     TTEFSGDAYG FNLVYSGNHA FELEKDQLDQ LHLMVGINSY NFNWQLKAGA TFQTPEVLMV
     YTNKGLNAMS QAYHHLIRER VVRSEFKNQE RPIVVNNWEA TFFDFNEAKL KPIVDEAKQL
     GIEMFVLDDG WFGHRDDDNS SLGDWQVDHR KFPQGLNHFV KYVHEQGLKF GIWLEPEMIS
     YDSKLYQQHP DYLMQVPGRS PSPSRNQYIL DLGRQAVRNN IFDQLDQLLK SKQIDYIKWD
     MNRHLSDIYS VALPPERQGE VYHRYVLGLY ELLERLTTAY PHILFEGCSG GGGRFDAGMA
     YYMPQIWASD NTDAVARLTI QYGTSLAYPI SLATAHVSVS PNQQTGRETS MSTRSAVAAS
     GVLGYELDLT QLSSADKQIV QKQVVQYKQI RPLIQFGEFY RLKSPITSNQ AAWMFVSPQQ
     DEAIVMVFNL TSYAQPSLTK TKLVGLNPKL NYQNIATKAI FGGDELMQLG FYDPVVYQDY
     TTKVYHFKAV TEN
//