ID   YMD8_YEAST              Reviewed;         442 AA.
AC   Q03697; D6VZD7;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   04-NOV-2026, entry version 173.
DE   RecName: Full=Putative nucleotide-sugar transporter YMD8;
GN   Name=YMD8; OrderedLocusNames=YML038C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872; DOI=10.1038/387s090;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:14562095}. Cytoplasmic vesicle, COPI-coated vesicle
CC       membrane {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- SIMILARITY: Belongs to the TPT transporter family. SLC35C subfamily.
CC       {ECO:0000305}.
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DR   EMBL; Z46659; CAA86617.1; -; Genomic_DNA.
DR   EMBL; AY692787; AAT92806.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA09861.1; -; Genomic_DNA.
DR   PIR; S49741; S49741.
DR   RefSeq; NP_013674.1; NM_001182396.1.
DR   AlphaFoldDB; Q03697; -.
DR   SMR; Q03697; -.
DR   BioGRID; 35132; 134.
DR   DIP; DIP-1312N; -.
DR   FunCoup; Q03697; 807.
DR   IntAct; Q03697; 11.
DR   MINT; Q03697; -.
DR   STRING; 4932.YML038C; -.
DR   TCDB; 2.A.7.9.14; the drug/metabolite transporter (dmt) superfamily.
DR   GlyCosmos; Q03697; 1 site, No reported glycans.
DR   GlyGen; Q03697; 1 site.
DR   iPTMnet; Q03697; -.
DR   PaxDb; 4932-YML038C; -.
DR   PeptideAtlas; Q03697; -.
DR   EnsemblFungi; YML038C_mRNA; YML038C; YML038C.
DR   GeneID; 854970; -.
DR   KEGG; sce:YML038C; -.
DR   AGR; SGD:S000004502; -.
DR   SGD; S000004502; YMD8.
DR   VEuPathDB; FungiDB:YML038C; -.
DR   eggNOG; KOG1443; Eukaryota.
DR   HOGENOM; CLU_022332_1_1_1; -.
DR   InParanoid; Q03697; -.
DR   OMA; AAMPFTE; -.
DR   OrthoDB; 18894at2759; -.
DR   BioCyc; YEAST:G3O-32637-MONOMER; -.
DR   BioGRID-ORCS; 854970; 0 hits in 10 CRISPR screens.
DR   PRO; PR:Q03697; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q03697; protein.
DR   GO; GO:0005801; C:cis-Golgi network; IBA:GO_Central.
DR   GO; GO:0030137; C:COPI-coated vesicle; HDA:SGD.
DR   GO; GO:0030663; C:COPI-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR   GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR   GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; ISS:SGD.
DR   GO; GO:0015780; P:nucleotide-sugar transmembrane transport; ISS:SGD.
DR   GO; GO:0015786; P:UDP-glucose transmembrane transport; IMP:SGD.
DR   InterPro; IPR037185; EmrE-like.
DR   InterPro; IPR013657; HUT1.
DR   InterPro; IPR050186; TPT_transporter.
DR   PANTHER; PTHR11132; SOLUTE CARRIER FAMILY 35; 1.
DR   Pfam; PF08449; UAA; 1.
DR   SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 2.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; Glycoprotein; Golgi apparatus; Membrane;
KW   Phosphoprotein; Reference proteome; Sugar transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..442
FT                   /note="Putative nucleotide-sugar transporter YMD8"
FT                   /id="PRO_0000213402"
FT   TOPO_DOM        1..3
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        25..32
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..76
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        98..107
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        129..132
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        154..166
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        188..254
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        276..301
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        323..329
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        351..355
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        377..442
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         209
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   CARBOHYD        99
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   442 AA;  49615 MW;  37A03543D3AB695E CRC64;
     MNRTVFLAFV FGWYFCSIAL SIYNRWMFDP KDGLGIGYPV LVTTFHQATL WLLSGIYIKL
     RHKPVKNVLR KNNGFNWSFF LKFLLPTAVA SAGDIGLSNV SFQYVPLTIY TIIKSSSIAF
     VLLFGCIFKL EKFHWKLALS VIIMFVGVAL MVFKPSDSTS TKNDQALVIF GSFLVLASSC
     LSGLRWVYTQ LMLRNNPIQT NTAAAVEESD GALFTENEDN VDNEPVVNLA NNKMLENFGE
     SKPHPIHTIH QLAPIMGITL LLTSLLVEKP FPGIFSSSIF RLDTSNGGVG TETTVLSIVR
     GIVLLILPGF AVFLLTICEF SILEQTPVLT VSIVGIVKEL LTVIFGIIIL SERLSGFYNW
     LGMLIIMADV CYYNYFRYKQ DLLQKYHSVS TQDNRNELKG FQDFEQLGSK KIAPYSISVD
     LTNQEYELDM IAQNVSRSSQ QV
//