ID   DHB4_RAT                Reviewed;         735 AA.
AC   P97852; P70523; P70540;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   04-NOV-2026, entry version 189.
DE   RecName: Full=Peroxisomal multifunctional enzyme type 2 {ECO:0000305};
DE            Short=MFE-2;
DE   AltName: Full=17-beta-hydroxysteroid dehydrogenase 4;
DE            Short=17-beta-HSD 4;
DE   AltName: Full=D-bifunctional protein;
DE            Short=DBP;
DE   AltName: Full=Multifunctional protein 2;
DE            Short=MFP-2;
DE   Contains:
DE     RecName: Full=(3R)-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.n12 {ECO:0000250|UniProtKB:P51659};
DE   Contains:
DE     RecName: Full=Enoyl-CoA hydratase 2;
DE              EC=4.2.1.107 {ECO:0000250|UniProtKB:P51659};
DE              EC=4.2.1.119 {ECO:0000250|UniProtKB:P51659};
DE     AltName: Full=3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase;
GN   Name=Hsd17b4 {ECO:0000312|RGD:621806}; Synonyms=Edh17b4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-30; 32-44; 110-115;
RP   132-139 AND 270-274, SUBCELLULAR LOCATION, AND PROTEOLYTIC CLEAVAGE.
RC   TISSUE=Liver;
RX   PubMed=8856068; DOI=10.1111/j.1432-1033.1996.0660h.x;
RA   Dieuaide-Noubhani M., Novikov D., Baumgart E., Vanhooren J.C.T.,
RA   Fransen M., Goethals M., Vandekerckhove J., Van Veldhoven P.P.,
RA   Mannaerts G.P.;
RT   "Further characterization of the peroxisomal 3-hydroxyacyl-CoA
RT   dehydrogenases from rat liver. Relationship between the different
RT   dehydrogenases and evidence that fatty acids and the C27 bile acids di- and
RT   tri-hydroxycoprostanic acids are metabolized by separate multifunctional
RT   proteins.";
RL   Eur. J. Biochem. 240:660-666(1996).
RN   [2]
RP   ERRATUM OF PUBMED:8856068.
RX   DOI=10.1111/j.1432-1033.1997.537_1a.x;
RA   Dieuaide-Noubhani M., Novikov D., Baumgart E., Vanhooren J.C.T.,
RA   Fransen M., Goethals M., Vandekerckhove J., Van Veldhoven P.P.,
RA   Mannaerts G.P.;
RL   Eur. J. Biochem. 243:537-537(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=8913347; DOI=10.1016/s0026-895x(25)09544-6;
RA   Corton J.C., Bocos C., Moreno E.S., Merritt A., Marsman D.S., Sausen P.J.,
RA   Cattley R.C., Gustafsson J.-A.;
RT   "Rat 17 beta-hydroxysteroid dehydrogenase type IV is a novel peroxisome
RT   proliferator-inducible gene.";
RL   Mol. Pharmacol. 50:1157-1166(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 318-330; 479-505; 542-554;
RP   562-576 AND 583-587, AND ENZYME CATALYSIS.
RX   PubMed=9003397; DOI=10.1042/bj3210021;
RA   Qin Y.M., Poutanen M.H., Helander H.M., Kvist A.P., Siivari K.M.,
RA   Schmitz W., Conzelmann E., Hellman U., Hiltunen J.K.;
RT   "Peroxisomal multifunctional enzyme of beta-oxidation metabolizing D-3-
RT   hydroxyacyl-CoA esters in rat liver: molecular cloning, expression and
RT   characterization.";
RL   Biochem. J. 321:21-28(1997).
RN   [5]
RP   PROTEIN SEQUENCE OF 312-324, AND PROTEOLYTIC CLEAVAGE.
RX   PubMed=9003427; DOI=10.1042/bj3210253;
RA   Dieuaide-Noubhani M., Novikov D., Vandekerckhove J., Veldhoven P.P.,
RA   Mannaerts G.P.;
RT   "Identification and characterization of the 2-enoyl-CoA hydratases involved
RT   in peroxisomal beta-oxidation in rat liver.";
RL   Biochem. J. 321:253-259(1997).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY IN COMPLEX WITH NADH, AND HOMODIMERIZATION.
RX   PubMed=12517343; DOI=10.1016/s0969-2126(02)00931-0;
RA   Haapalainen A.M., Koski M.K., Qin Y.-M., Hiltunen J.K., Glumoff T.;
RT   "Binary structure of the two-domain (3R)-hydroxyacyl-CoA dehydrogenase from
RT   rat peroxisomal multifunctional enzyme type 2 at 2.38 A resolution.";
RL   Structure 11:87-97(2003).
CC   -!- FUNCTION: Bifunctional enzyme acting on the peroxisomal fatty acid
CC       beta-oxidation pathway. Catalyzes two of the four reactions in fatty
CC       acid degradation: hydration of 2-enoyl-CoA (trans-2-enoyl-CoA) to
CC       produce (3R)-3-hydroxyacyl-CoA, and dehydrogenation of (3R)-3-
CC       hydroxyacyl-CoA to produce 3-ketoacyl-CoA (3-oxoacyl-CoA), which is
CC       further metabolized by SCPx. Can use straight-chain and branched-chain
CC       fatty acids, as well as bile acid intermediates as substrates. May play
CC       a role in peroxisomal beta-oxidation step in polyunsaturated fatty
CC       acids (PUFAs) biosynthesis. Possibly regulates systemic levels of
CC       docosahexaenoic acid (DHA, C22:6n-3) through a process involving
CC       endoplasmic reticulum desaturation and elongation of alpha-linolenic
CC       acid (ALA, C18:3n-3) to form tetracosahexaenoic acid (THA, C24:6n-3),
CC       which is then beta-oxidized to DHA in peroxisomes.
CC       {ECO:0000250|UniProtKB:P51659}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:26526, ChEBI:CHEBI:15377, ChEBI:CHEBI:57319,
CC         ChEBI:CHEBI:58856; EC=4.2.1.119;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:26528;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH +
CC         H(+); Xref=Rhea:RHEA:32711, ChEBI:CHEBI:15378, ChEBI:CHEBI:57319,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726;
CC         EC=1.1.1.n12; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32712;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain
CC         (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:45812, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:83728, ChEBI:CHEBI:85440;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45814;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytriacontanoyl-CoA = (2E)-triacontenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39235, ChEBI:CHEBI:15377, ChEBI:CHEBI:76384,
CC         ChEBI:CHEBI:76385; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39237;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyoctacosanoyl-CoA = (2E)-octacosenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39223, ChEBI:CHEBI:15377, ChEBI:CHEBI:76382,
CC         ChEBI:CHEBI:76383; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39225;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyhexacosanoyl-CoA = (2E)-hexacosenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39211, ChEBI:CHEBI:15377, ChEBI:CHEBI:74281,
CC         ChEBI:CHEBI:76378; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39213;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytetracosanoyl-CoA = (2E)-tetracosenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39199, ChEBI:CHEBI:15377, ChEBI:CHEBI:74693,
CC         ChEBI:CHEBI:76377; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39201;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,17Z)-hexacosadienoyl-CoA + H2O = (3R)-hydroxy-(17Z)-
CC         hexacosenoyl-CoA; Xref=Rhea:RHEA:83119, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:232757, ChEBI:CHEBI:232758;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83120;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(15Z)-tetracosenoyl-CoA = (2E,15Z)-
CC         tetracosadienoyl-CoA + H2O; Xref=Rhea:RHEA:39271, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:76392, ChEBI:CHEBI:76393;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39273;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6Z,9Z,12Z,15Z,18Z)-tetracosahexaenoyl-CoA + H2O = (3R)-
CC         hydroxy-(6Z,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA;
CC         Xref=Rhea:RHEA:84379, ChEBI:CHEBI:15377, ChEBI:CHEBI:76364,
CC         ChEBI:CHEBI:233764; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84380;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6Z,9Z,12Z,15Z,18Z,21Z)-tetracosaheptaenoyl-CoA + H2O =
CC         (3R)-hydroxy-(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA;
CC         Xref=Rhea:RHEA:79187, ChEBI:CHEBI:15377, ChEBI:CHEBI:76360,
CC         ChEBI:CHEBI:229700; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:79188;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a very-long-chain (3R)-3-hydroxyacyl-CoA + NAD(+) = a very-
CC         long-chain 3-oxoacyl-CoA + NADH + H(+); Xref=Rhea:RHEA:78871,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:85440, ChEBI:CHEBI:90725;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78872;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytriacontanoyl-CoA + NAD(+) = 3-oxo-triacontanoyl-
CC         CoA + NADH + H(+); Xref=Rhea:RHEA:85335, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:74228,
CC         ChEBI:CHEBI:76384; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:85336;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyoctacosanoyl-CoA + NAD(+) = 3-oxooctacosanyol-CoA
CC         + NADH + H(+); Xref=Rhea:RHEA:85339, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:73976,
CC         ChEBI:CHEBI:76382; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:85340;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-oxohexacosanoyl-CoA + NADH + H(+) = (3R)-
CC         hydroxyhexacosanoyl-CoA + NAD(+); Xref=Rhea:RHEA:78635,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:73980, ChEBI:CHEBI:76378;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78637;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytetracosanoyl-CoA + NAD(+) = 3-oxotetracosanoyl-
CC         CoA + NADH + H(+); Xref=Rhea:RHEA:78563, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:73977,
CC         ChEBI:CHEBI:76377; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78564;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(17Z)-hexacosenoyl-CoA + NAD(+) = 3-oxo-(17Z)-
CC         hexacosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:83123,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:232757, ChEBI:CHEBI:232759;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:83124;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(15Z)-tetracosenoyl-CoA + NAD(+) = 3-oxo-(15Z)-
CC         tetracosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86175,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:74071, ChEBI:CHEBI:76392;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86176;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(6Z,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA +
CC         NAD(+) = 3-oxo-(6Z,9Z,12Z,15Z,18Z)-tetracosapentaenoyl-CoA + NADH +
CC         H(+); Xref=Rhea:RHEA:84383, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:76367, ChEBI:CHEBI:233764;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84384;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA +
CC         NAD(+) = 3-oxo-(6Z,9Z,12Z,15Z,18Z,21Z)-tetracosahexaenoyl-CoA + NADH
CC         + H(+); Xref=Rhea:RHEA:79191, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:74304, ChEBI:CHEBI:229700;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:79192;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain (3R)-hydroxyacyl-CoA = a long-chain (2E)-enoyl-
CC         CoA + H2O; Xref=Rhea:RHEA:78931, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:83727, ChEBI:CHEBI:229602;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78933;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxydocosanoyl-CoA = (2E)-docosenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39187, ChEBI:CHEBI:15377, ChEBI:CHEBI:74692,
CC         ChEBI:CHEBI:76375; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39189;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyeicosanoyl-CoA = (2E)-eicosenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39175, ChEBI:CHEBI:15377, ChEBI:CHEBI:74691,
CC         ChEBI:CHEBI:76373; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39177;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyoctadecanoyl-CoA = (2E)-octadecenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39155, ChEBI:CHEBI:15377, ChEBI:CHEBI:71412,
CC         ChEBI:CHEBI:76374; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39157;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39159, ChEBI:CHEBI:15377, ChEBI:CHEBI:61526,
CC         ChEBI:CHEBI:74278; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39161;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytetradecanoyl-CoA = (2E)-tetradecenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:78643, ChEBI:CHEBI:15377, ChEBI:CHEBI:61405,
CC         ChEBI:CHEBI:74277; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78645;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(13Z)-docosenoyl-CoA = (2E,13Z)-docosadienoyl-CoA
CC         + H2O; Xref=Rhea:RHEA:39259, ChEBI:CHEBI:15377, ChEBI:CHEBI:76389,
CC         ChEBI:CHEBI:76391; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39261;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(11Z)-eicosenoyl-CoA = (2E,11Z)-eicosadienoyl-CoA
CC         + H2O; Xref=Rhea:RHEA:39247, ChEBI:CHEBI:15377, ChEBI:CHEBI:76387,
CC         ChEBI:CHEBI:76388; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39249;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,9Z)-octadecadienoyl-CoA + H2O = (3R)-hydroxy-(9Z)-
CC         octadecenoyl-CoA; Xref=Rhea:RHEA:86491, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:77553, ChEBI:CHEBI:235452;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86492;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,7Z)-hexadecadienoyl-CoA + H2O = (3R)-hydroxy-(7Z)-
CC         hexadecenoyl-CoA; Xref=Rhea:RHEA:86587, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:87717, ChEBI:CHEBI:235453;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86588;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,5Z)-tetradecadienoyl-CoA + H2O = (3R)-hydroxy-(5Z)-
CC         tetradecenoyl-CoA; Xref=Rhea:RHEA:86595, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:87701, ChEBI:CHEBI:235454;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86596;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,11Z)-docosadienoyl-CoA + H2O = (3R)-hydroxy-(11Z)-
CC         docosenoyl-CoA; Xref=Rhea:RHEA:87091, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:232888, ChEBI:CHEBI:235443;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:87092;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,9Z)-eicosadienoyl-CoA + H2O = (3R)-hydroxy-(9Z)-
CC         eicosenoyl-CoA; Xref=Rhea:RHEA:87095, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:232892, ChEBI:CHEBI:235451;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:87096;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,7Z)-octadecadienoyl-CoA + H2O = (3R)-hydroxy-(7Z)-
CC         octadecenoyl-CoA; Xref=Rhea:RHEA:87111, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:232899, ChEBI:CHEBI:235455;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:87112;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,5Z)-hexadecadienoyl-CoA + H2O = (3R)-hydroxy-(5Z)-
CC         hexadecenoyl-CoA; Xref=Rhea:RHEA:87115, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:232910, ChEBI:CHEBI:235456;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:87116;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z,13Z,16Z)-docosatetraenoyl-CoA =
CC         (2E,7Z,10Z,13Z,16Z)-docosapentaenoyl-CoA + H2O; Xref=Rhea:RHEA:39327,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:76415, ChEBI:CHEBI:76416;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39329;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA =
CC         (2E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:39463, ChEBI:CHEBI:15377, ChEBI:CHEBI:76460,
CC         ChEBI:CHEBI:76461; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39465;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(8Z,11Z,14Z)-eicosatrienoyl-CoA =
CC         (2E,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O; Xref=Rhea:RHEA:39315,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:76411, ChEBI:CHEBI:76412;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39317;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA =
CC         (2E,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + H2O; Xref=Rhea:RHEA:39451,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:76457, ChEBI:CHEBI:76458;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:39453;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6Z,9Z,12Z)-octadecatetraenoyl-CoA + H2O = (3R)-hydroxy-
CC         (6Z,9Z,12Z)-octadecatrienoyl-CoA; Xref=Rhea:RHEA:84915,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:233778, ChEBI:CHEBI:233779;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84916;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,6Z,9Z,12Z,15Z)-octadecapentaenoyl-CoA + H2O = 3R-hydroxy-
CC         (6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA; Xref=Rhea:RHEA:84807,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:144032, ChEBI:CHEBI:233740;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84808;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,7Z,10Z)-hexadecatrienoyl-CoA + H2O = (3R)-hydroxy-
CC         (7Z,10Z)-hexadecadienoyl-CoA; Xref=Rhea:RHEA:84919,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:233785, ChEBI:CHEBI:233786;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84920;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,7Z,10Z,13Z)-hexadecatetraenoyl-CoA + H2O = (3R)-hydroxy-
CC         (7Z,10Z,13Z)-hexadecatrienoyl-CoA; Xref=Rhea:RHEA:84811,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:233792, ChEBI:CHEBI:233793;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84812;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E,8Z,11Z)-tetradecatrienoyl-CoA + H2O = (3R)-hydroxy-
CC         (8Z,11Z)-tetradecadienoyl-CoA; Xref=Rhea:RHEA:84823,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:233801, ChEBI:CHEBI:233802;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84824;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain (3R)-hydroxyacyl-CoA + NAD(+) = a long-chain 3-
CC         oxo-fatty acyl-CoA + NADH + H(+); Xref=Rhea:RHEA:78935,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:136758, ChEBI:CHEBI:229602;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78936;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxydocosanoyl-CoA + NAD(+) = 3-oxodocosanoyl-CoA +
CC         NADH + H(+); Xref=Rhea:RHEA:78579, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71451,
CC         ChEBI:CHEBI:76375; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78580;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyeicosanoyl-CoA + NAD(+) = 3-oxoeicosanoyl-CoA +
CC         NADH + H(+); Xref=Rhea:RHEA:78595, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65115,
CC         ChEBI:CHEBI:76373; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78596;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyoctadecanoyl-CoA + NAD(+) = 3-oxooctadecanoyl-CoA
CC         + NADH + H(+); Xref=Rhea:RHEA:78607, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71407,
CC         ChEBI:CHEBI:76374; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78608;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA
CC         + NADH + H(+); Xref=Rhea:RHEA:40243, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57349, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:74278; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40244;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxytetradecanoyl-CoA + NAD(+) = 3-oxotetradecanoyl-
CC         CoA + NADH + H(+); Xref=Rhea:RHEA:78667, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62543,
CC         ChEBI:CHEBI:74277; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78668;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(13Z)-docosenoyl-CoA + NAD(+) = 3-oxo-(13Z)-
CC         docosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86171,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:74070, ChEBI:CHEBI:76389;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86172;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(11Z)-eicosenoyl-CoA + NAD(+) = 3-oxo-(11Z)-
CC         eicosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86179,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:74011, ChEBI:CHEBI:76387;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86180;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(9Z)-octadecenoyl-CoA + NAD(+) = 3-oxo-(9Z-
CC         octadecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:86183,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:87695, ChEBI:CHEBI:235452;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86184;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z)-hexadecenoyl-CoA + NAD(+) = 3-oxo-(7Z-
CC         hexadecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:86187,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:87719, ChEBI:CHEBI:235453;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86188;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(5Z)-tetradecenoyl-CoA + NAD(+) = 3-oxo-(5Z-
CC         tetradecenoyl)-CoA + NADH + H(+); Xref=Rhea:RHEA:86191,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:87707, ChEBI:CHEBI:235454;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86192;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(11Z)-docosenoyl-CoA + NAD(+) = 3-oxo-(11Z)-
CC         docosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86195,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:232890, ChEBI:CHEBI:235443;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86196;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(9Z)-eicosenoyl-CoA + NAD(+) = 3-oxo-(9Z)-
CC         eicosenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86199,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:232898, ChEBI:CHEBI:235451;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86200;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z)-octadecenoyl-CoA + NAD(+) = 3-oxo-(7Z)-
CC         octadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86203,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:232901, ChEBI:CHEBI:235455;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86204;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(5Z)-hexadecenoyl-CoA + NAD(+) = 3-oxo-(5Z)-
CC         hexadecenoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:86207,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:232912, ChEBI:CHEBI:235456;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:86208;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z,13Z,16Z)-docosatetraenoyl-CoA + NAD(+) =
CC         (7Z,10Z,13Z,16Z)-3-oxodocosatetraenoyl-CoA + NADH + H(+);
CC         Xref=Rhea:RHEA:84387, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:73852, ChEBI:CHEBI:76415;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84388;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA +
CC         NAD(+) = 3-oxo-(7Z,10Z,13Z,16Z,19Z)-docosapentaenoyl-CoA + NADH +
CC         H(+); Xref=Rhea:RHEA:84307, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:73863, ChEBI:CHEBI:76460;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84308;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(8Z,11Z,14Z)-eicosatrienoyl-CoA + NAD(+) = 3-oxo-
CC         (8Z,11Z,14Z)-eicosatrienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84867,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:71481, ChEBI:CHEBI:76411;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84868;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA + NAD(+) =
CC         3-oxo-(8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA + NADH + H(+);
CC         Xref=Rhea:RHEA:84795, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:71491, ChEBI:CHEBI:76457;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84796;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(6Z,9Z,12Z)-octadecatrienoyl-CoA + NAD(+) = 3-
CC         oxo-(6Z,9Z,12Z)-octadecatrienoyl-CoA + NADH + H(+);
CC         Xref=Rhea:RHEA:84871, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:233779, ChEBI:CHEBI:233780;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84872;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3R-hydroxy-(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + NAD(+) =
CC         3-oxo-(6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + NADH + H(+);
CC         Xref=Rhea:RHEA:84803, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:144032, ChEBI:CHEBI:233746;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84804;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z)-hexadecadienoyl-CoA + NAD(+) = 3-oxo-
CC         (7Z,10Z)-hexadecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84875,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:233786, ChEBI:CHEBI:233788;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84876;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(7Z,10Z,13Z)-hexadecatrienoyl-CoA + NAD(+) = 3-
CC         oxo-(7Z,10Z,13Z)-hexadecatrienoyl-CoA + NADH + H(+);
CC         Xref=Rhea:RHEA:84815, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:233793, ChEBI:CHEBI:233794;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84816;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxy-(8Z,11Z)-tetradecadienoyl-CoA + NAD(+) = 3-oxo-
CC         (8Z,11Z)-tetradecadienoyl-CoA + NADH + H(+); Xref=Rhea:RHEA:84831,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:233802, ChEBI:CHEBI:233803;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:84832;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a medium-chain (3R)-hydroxyacyl-CoA = a medium-chain (2E)-
CC         enoyl-CoA + H2O; Xref=Rhea:RHEA:78867, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:83726, ChEBI:CHEBI:229603;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:78869;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-3-hydroxydodecanoyl-CoA = (2E)-dodecenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:44024, ChEBI:CHEBI:15377, ChEBI:CHEBI:57330,
CC         ChEBI:CHEBI:74276; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:44026;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-3-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O;
CC         Xref=Rhea:RHEA:45992, ChEBI:CHEBI:15377, ChEBI:CHEBI:61406,
CC         ChEBI:CHEBI:74272; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:45994;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-octenoyl-CoA + H2O = (3R)-hydroxyoctanoyl-CoA;
CC         Xref=Rhea:RHEA:40187, ChEBI:CHEBI:15377, ChEBI:CHEBI:62242,
CC         ChEBI:CHEBI:74279; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40188;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a medium-chain (3R)-hydroxyacyl-CoA + NAD(+) = a medium-chain
CC         3-oxoacyl-CoA + NADH + H(+); Xref=Rhea:RHEA:78875, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:229603,
CC         ChEBI:CHEBI:229604; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78876;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-3-hydroxydodecanoyl-CoA + NAD(+) = 3-oxododecanoyl-CoA +
CC         NADH + H(+); Xref=Rhea:RHEA:78671, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62615,
CC         ChEBI:CHEBI:74276; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:78672;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-3-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH
CC         + H(+); Xref=Rhea:RHEA:45832, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:62548, ChEBI:CHEBI:74272;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45833;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH +
CC         H(+); Xref=Rhea:RHEA:40191, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:62619, ChEBI:CHEBI:74279;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40192;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2E)-hexadecenedioyl-CoA + H2O = (3R)-hydroxyhexadecanedioyl-
CC         CoA; Xref=Rhea:RHEA:40255, ChEBI:CHEBI:15377, ChEBI:CHEBI:77075,
CC         ChEBI:CHEBI:77079; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40256;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-hydroxyhexadecanedioyl-CoA + NAD(+) = 3-
CC         oxohexadecanedioyl-CoA + NADH + H(+); Xref=Rhea:RHEA:40263,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:77079, ChEBI:CHEBI:77081;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40264;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-
CC         cholestan-26-oyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-
CC         cholest-24-en-26-oyl-CoA + H2O; Xref=Rhea:RHEA:18933,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:59807, ChEBI:CHEBI:59879;
CC         EC=4.2.1.107; Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:18935;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-
CC         cholestan-26-oyl-CoA + NAD(+) = 3alpha,7alpha,12alpha-trihydroxy-24-
CC         oxo-5beta-cholestan-26-oyl-CoA + NADH + H(+); Xref=Rhea:RHEA:47088,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC         ChEBI:CHEBI:58507, ChEBI:CHEBI:59807;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:47089;
CC         Evidence={ECO:0000250|UniProtKB:P51659};
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC       {ECO:0000250|UniProtKB:P51659}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12517343}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000269|PubMed:8856068}.
CC   -!- MISCELLANEOUS: The protein is found both as a full-length peptide and
CC       in a cleaved version.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- CAUTION: Peroxisomal beta-oxidation of VLCFAs is likely partial,
CC       enabling formation of chain-shortened fatty acids that are further
CC       esterified in endoplasmic reticulum to form membrane lipids or routed
CC       to mitochondria for full oxidation and ATP production. The experimental
CC       data are scarce and some reactions in this entry are inferred from
CC       combined genetic interaction or mutant phenotype evidence and direct
CC       experimental evidence in parent reaction.
CC       {ECO:0000250|UniProtKB:P51659}.
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DR   EMBL; S83279; AAB49519.1; -; mRNA.
DR   EMBL; U37486; AAB09724.1; -; mRNA.
DR   EMBL; X94978; CAA64427.1; -; mRNA.
DR   RefSeq; NP_077368.2; NM_024392.2.
DR   PDB; 1GZ6; X-ray; 2.38 A; A/B/C/D=1-319.
DR   AlphaFoldDB; P97852; -.
DR   SMR; P97852; -.
DR   BioGRID; 249442; 2.
DR   FunCoup; P97852; 1335.
DR   IntAct; P97852; 2.
DR   STRING; 10116.ENSRNOP00000070792; -.
DR   ChEMBL; CHEMBL1075219; -.
DR   GlyGen; P97852; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; P97852; -.
DR   PhosphoSitePlus; P97852; -.
DR   jPOST; P97852; -.
DR   PaxDb; 10116-ENSRNOP00000070792; -.
DR   Ensembl; ENSRNOT00000115693.2; ENSRNOP00000081103.2; ENSRNOG00000015840.9.
DR   Ensembl; ENSRNOT00060021465; ENSRNOP00060016963; ENSRNOG00060012603.
DR   Ensembl; ENSRNOT00065005980; ENSRNOP00065004285; ENSRNOG00065004039.
DR   GeneID; 79244; -.
DR   KEGG; rno:79244; -.
DR   UCSC; RGD:621806; rat.
DR   AGR; RGD:621806; -.
DR   CTD; 3295; -.
DR   RGD; 621806; Hsd17b4.
DR   VEuPathDB; HostDB:ENSRNOG00000015840; -.
DR   eggNOG; ENOG502QPX4; Eukaryota.
DR   GeneTree; ENSGT00940000158343; -.
DR   HOGENOM; CLU_010194_18_4_1; -.
DR   InParanoid; P97852; -.
DR   OrthoDB; 3592703at2759; -.
DR   BioCyc; MetaCyc:MONOMER-14329; -.
DR   Reactome; R-RNO-193368; Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
DR   Reactome; R-RNO-2046106; alpha-linolenic acid (ALA) metabolism.
DR   Reactome; R-RNO-389887; Beta-oxidation of pristanoyl-CoA.
DR   Reactome; R-RNO-390247; Beta-oxidation of very long chain fatty acids.
DR   Reactome; R-RNO-9033241; Peroxisomal protein import.
DR   UniPathway; UPA00659; -.
DR   EvolutionaryTrace; P97852; -.
DR   PRO; PR:P97852; -.
DR   Proteomes; UP000002494; Chromosome 18.
DR   Bgee; ENSRNOG00000015840; Expressed in liver and 19 other cell types or tissues.
DR   ExpressionAtlas; P97852; baseline and differential.
DR   GO; GO:0005782; C:peroxisomal matrix; ISO:RGD.
DR   GO; GO:0005777; C:peroxisome; IDA:HGNC-UCL.
DR   GO; GO:0106386; F:(3R)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity; ISS:UniProtKB.
DR   GO; GO:0003857; F:(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity; ISO:RGD.
DR   GO; GO:0044594; F:17-beta-hydroxysteroid dehydrogenase (NAD+) activity; IDA:RGD.
DR   GO; GO:0018812; F:3-hydroxyacyl-CoA dehydratase activity; IDA:RGD.
DR   GO; GO:0033989; F:3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; ISS:UniProtKB.
DR   GO; GO:0004303; F:estradiol 17-beta-dehydrogenase [NAD(P)+] activity; IDA:RGD.
DR   GO; GO:0042802; F:identical protein binding; IPI:RGD.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR   GO; GO:0102158; F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity; IEA:RHEA.
DR   GO; GO:0008209; P:androgen metabolic process; ISO:RGD.
DR   GO; GO:0008210; P:estrogen metabolic process; ISO:RGD.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; ISS:UniProtKB.
DR   GO; GO:0033540; P:fatty acid beta-oxidation using acyl-CoA oxidase; ISO:RGD.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; ISO:RGD.
DR   GO; GO:0036112; P:medium-chain fatty-acyl-CoA metabolic process; ISO:RGD.
DR   GO; GO:0048545; P:response to steroid hormone; IEP:RGD.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR   GO; GO:0060009; P:Sertoli cell development; ISO:RGD.
DR   GO; GO:0000038; P:very long-chain fatty acid metabolic process; ISO:RGD.
DR   GO; GO:0036111; P:very long-chain fatty-acyl-CoA metabolic process; ISO:RGD.
DR   CDD; cd03448; HDE_HSD; 1.
DR   CDD; cd05353; hydroxyacyl-CoA-like_DH_SDR_c-like; 1.
DR   FunFam; 3.30.1050.10:FF:000004; Hydroxysteroid 17-beta dehydrogenase 4; 1.
DR   FunFam; 1.10.287.4290:FF:000001; Peroxisomal multifunctional enzyme type 2; 1.
DR   FunFam; 3.10.129.10:FF:000013; Peroxisomal multifunctional enzyme type 2; 1.
DR   FunFam; 3.10.129.10:FF:000019; peroxisomal multifunctional enzyme type 2; 1.
DR   FunFam; 3.40.50.720:FF:000185; peroxisomal multifunctional enzyme type 2; 1.
DR   Gene3D; 1.10.287.4290; -; 1.
DR   Gene3D; 3.10.129.10; Hotdog Thioesterase; 2.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   Gene3D; 3.30.1050.10; SCP2 sterol-binding domain; 1.
DR   InterPro; IPR029069; HotDog_dom_sf.
DR   InterPro; IPR057326; KR_dom.
DR   InterPro; IPR002539; MaoC-like_dom.
DR   InterPro; IPR054357; MFE-2_N.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR051687; Peroxisomal_Beta-Oxidation.
DR   InterPro; IPR003033; SCP2_sterol-bd_dom.
DR   InterPro; IPR036527; SCP2_sterol-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PANTHER; PTHR45024; DEHYDROGENASES, SHORT CHAIN; 1.
DR   PANTHER; PTHR45024:SF2; SCP2 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   Pfam; PF01575; MaoC_dehydratas; 1.
DR   Pfam; PF22622; MFE-2_hydrat-2_N; 1.
DR   Pfam; PF02036; SCP2; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SMART; SM00822; PKS_KR; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   SUPFAM; SSF55718; SCP-like; 1.
DR   SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Direct protein sequencing;
KW   Fatty acid metabolism; Isomerase; Lipid metabolism; Lyase; NAD;
KW   Oxidoreductase; Peroxisome; Phosphoprotein; Reference proteome.
FT   CHAIN           1..735
FT                   /note="Peroxisomal multifunctional enzyme type 2"
FT                   /id="PRO_0000054585"
FT   CHAIN           1..311
FT                   /note="(3R)-hydroxyacyl-CoA dehydrogenase"
FT                   /id="PRO_0000400086"
FT   CHAIN           312..735
FT                   /note="Enoyl-CoA hydratase 2"
FT                   /id="PRO_0000400087"
FT   DOMAIN          483..599
FT                   /note="MaoC-like"
FT   DOMAIN          623..735
FT                   /note="SCP2"
FT   REGION          1..305
FT                   /note="(3R)-hydroxyacyl-CoA dehydrogenase"
FT   REGION          321..621
FT                   /note="Enoyl-CoA hydratase 2"
FT   MOTIF           733..735
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT   BINDING         21
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         40
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         75
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         76
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         99
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         101
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         164
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         168
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         196
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         198
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         199
FT                   /ligand="NADH"
FT                   /ligand_id="ChEBI:CHEBI:57945"
FT                   /evidence="ECO:0000269|PubMed:12517343,
FT                   ECO:0007744|PDB:1GZ6"
FT   BINDING         405..406
FT                   /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:74272"
FT                   /evidence="ECO:0000250|UniProtKB:P22414"
FT   BINDING         434
FT                   /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:74272"
FT                   /evidence="ECO:0000250|UniProtKB:P22414"
FT   BINDING         509..514
FT                   /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:74272"
FT                   /evidence="ECO:0000250|UniProtKB:P22414"
FT   BINDING         532
FT                   /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:74272"
FT                   /evidence="ECO:0000250|UniProtKB:P22414"
FT   BINDING         562
FT                   /ligand="(3R)-3-hydroxydecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:74272"
FT                   /evidence="ECO:0000250|UniProtKB:P22414"
FT   BINDING         705
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         723
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         46
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         46
FT                   /note="N6-succinyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         52
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         57
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         68
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         84
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         265
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         275
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         304
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         308
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         355
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         423
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         564
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         578
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         662
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   MOD_RES         668
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51659"
FT   MOD_RES         724
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:P51660"
FT   CONFLICT        1..2
FT                   /note="MA -> M (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        12
FT                   /note="V -> G (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        26
FT                   /note="A -> G (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        76
FT                   /note="V -> G (in Ref. 4; AAB09724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        106
FT                   /note="R -> P (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        157
FT                   /note="G -> S (in Ref. 4; AAB09724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        284
FT                   /note="K -> E (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        368
FT                   /note="A -> P (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        376
FT                   /note="F -> I (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        386
FT                   /note="M -> T (in Ref. 4; AAB09724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        485
FT                   /note="S -> D (in Ref. 4; AAB09724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        498
FT                   /note="L -> V (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        521
FT                   /note="S -> G (in Ref. 1; CAA64427)"
FT                   /evidence="ECO:0000305"
FT   STRAND          11..14
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   TURN            15..18
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           20..31
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          35..39
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           53..64
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          68..72
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           76..78
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           79..89
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          95..98
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           108..110
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           113..120
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           121..125
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           126..141
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          144..149
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           152..156
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           162..181
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           183..185
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          187..195
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   TURN            199..201
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           202..204
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           207..212
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           215..217
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           219..225
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          236..240
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   STRAND          243..251
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           266..271
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           273..276
FT                   /evidence="ECO:0007829|PDB:1GZ6"
FT   HELIX           288..302
FT                   /evidence="ECO:0007829|PDB:1GZ6"
SQ   SEQUENCE   735 AA;  79428 MW;  E14F15FFD35FC7D8 CRC64;
     MASPLRFDGR VVLVTGAGGG LGRAYALAFA ERGALVVVND LGGDFKGVGK GSSAADKVVE
     EIRRRGGKAV ANYDSVEAGE KLVKTALDTF GRIDVVVNNA GILRDRSFSR ISDEDWDIIQ
     RVHLRGSFQV TRAAWDHMKK QNYGRIIMTA SASGIYGNFG QANYSAAKLG LLGLANTLVI
     EGRKNNIHCN TIAPNAGSRM TETVMPEDLV EALKPEYVAP LVLWLCHESC EENGGLFEVG
     AGWIGKLRWE RTLGAIVRKR NQPMTPEAVR DNWVKICDFS NASKPKSIQE STGGIIEVLH
     KIDSEGISQN HTGQVASADA SGFAGVVGHK LPSFSSSYTE LQCIMYALGV GASVKNPKDL
     KFVYEGSADF SCLPTFGVIV AQKSLMSGGL AEVPGLSINF AKVLHGEQYL ELYKPLPRSG
     ELKCEAVIAD ILDKGSGIVI VMDVYSYSGK ELICYNQFSV FVVGSGGFGG KRTSEKLKAA
     VAVPSRPPDA VLRDTTSLNQ AALYRLSGDS NPLHIDPSFA SIAGFEKPIL HGLCTFGFSA
     RHVLQQFADN DVSRFKAIKV RFAKPVYPGQ TLQTEMWKEG NRIHFQTKVQ ETGDIVISNA
     YVDLVPTSGV SAQTPSEGGA LQSALVFGEI GRRLKDVGRE VVKKVNAVFE WHITKNGNVA
     AKWTIDLKNG SGEVYQGPAK GSADTTITIS DEDFMEVVLG KLNPQNAFFS GRLKARGNIM
     LSQKLQMILK DYAKL
//