ID KCNJ8_MOUSE Reviewed; 424 AA. AC P97794; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1997, sequence version 1. DT 02-SEP-2026, entry version 188. DE RecName: Full=ATP-sensitive inward rectifier potassium channel 8; DE AltName: Full=Inward rectifier K(+) channel Kir6.1; DE AltName: Full=Potassium channel, inwardly rectifying subfamily J member 8; DE AltName: Full=uKATP-1; GN Name=Kcnj8; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND INTERACTION WITH ABCC9. RC TISSUE=Brain; RX PubMed=9130167; DOI=10.1113/jphysiol.1997.sp021963; RA Yamada M., Isomoto S., Matsumoto S., Kondo C., Shindo T., Horio Y., RA Kurachi Y.; RT "Sulphonylurea receptor 2B and Kir6.1 form a sulphonylurea-sensitive but RT ATP-insensitive K+ channel."; RL J. Physiol. (Lond.) 499:715-720(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J; TISSUE=Mammary gland; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Heart, Kidney, and Spleen; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: Inward rectifier potassium channels are characterized by a CC greater tendency to allow potassium to flow into the cell rather than CC out of it. Their voltage dependence is regulated by the concentration CC of extracellular potassium; as external potassium is raised, the CC voltage range of the channel opening shifts to more positive voltages. CC The inward rectification is mainly due to the blockage of outward CC current by internal magnesium. This channel is activated by internal CC ATP and can be blocked by external barium (By similarity). Can form a CC sulfonylurea-sensitive but ATP-insensitive potassium channel with ABCC9 CC (PubMed:9130167). {ECO:0000250|UniProtKB:Q15842, CC ECO:0000269|PubMed:9130167}. CC -!- CATALYTIC ACTIVITY: CC Reaction=K(+)(in) = K(+)(out); Xref=Rhea:RHEA:29463, ChEBI:CHEBI:29103; CC Evidence={ECO:0000250|UniProtKB:Q15842}; CC -!- SUBUNIT: Interacts with ABCC9. {ECO:0000269|PubMed:9130167}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane CC protein {ECO:0000255}. CC -!- SIMILARITY: Belongs to the inward rectifier-type potassium channel CC (TC 1.A.2.1) family. KCNJ8 subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D88159; BAA13550.1; -; mRNA. DR EMBL; BC003756; AAH03756.1; -; mRNA. DR CCDS; CCDS20685.1; -. DR RefSeq; NP_001317292.1; NM_001330363.1. DR RefSeq; NP_032454.1; NM_008428.5. DR AlphaFoldDB; P97794; -. DR SMR; P97794; -. DR CORUM; P97794; -. DR FunCoup; P97794; 179. DR STRING; 10090.ENSMUSP00000145440; -. DR iPTMnet; P97794; -. DR PhosphoSitePlus; P97794; -. DR PaxDb; 10090-ENSMUSP00000145440; -. DR ProteomicsDB; 268960; -. DR ABCD; P97794; 2 sequenced antibodies. DR Antibodypedia; 4014; 264 antibodies from 32 providers. DR DNASU; 16523; -. DR Ensembl; ENSMUST00000203945.3; ENSMUSP00000145440.2; ENSMUSG00000030247.10. DR GeneID; 16523; -. DR KEGG; mmu:16523; -. DR UCSC; uc009epk.1; mouse. DR AGR; MGI:1100508; -. DR CTD; 3764; -. DR MGI; MGI:1100508; Kcnj8. DR VEuPathDB; HostDB:ENSMUSG00000030247; -. DR eggNOG; KOG3827; Eukaryota. DR GeneTree; ENSGT01140000282491; -. DR HOGENOM; CLU_022738_4_0_1; -. DR InParanoid; P97794; -. DR OMA; RMITEHC; -. DR OrthoDB; 273257at2759; -. DR PhylomeDB; P97794; -. DR Reactome; R-MMU-1296025; ATP sensitive Potassium channels. DR BioGRID-ORCS; 16523; 1 hit in 77 CRISPR screens. DR PRO; PR:P97794; -. DR Proteomes; UP000000589; Chromosome 6. DR RNAct; P97794; protein. DR Bgee; ENSMUSG00000030247; Expressed in brain blood vessel and 176 other cell types or tissues. DR ExpressionAtlas; P97794; baseline and differential. DR GO; GO:0098978; C:glutamatergic synapse; ISO:GO_Central. DR GO; GO:0008282; C:inward rectifying potassium channel; IGI:MGI. DR GO; GO:0030016; C:myofibril; IDA:MGI. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0098793; C:presynapse; IDA:MGI. DR GO; GO:0048787; C:presynaptic active zone membrane; ISO:GO_Central. DR GO; GO:0042383; C:sarcolemma; ISO:GO_Central. DR GO; GO:0005524; F:ATP binding; ISO:GO_Central. DR GO; GO:0015272; F:ATP-activated inward rectifier potassium channel activity; ISS:UniProtKB. DR GO; GO:0019829; F:ATPase-coupled monoatomic cation transmembrane transporter activity; ISO:GO_Central. DR GO; GO:0017098; F:sulfonylurea receptor binding; ISO:GO_Central. DR GO; GO:0099508; F:voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential; ISO:GO_Central. DR GO; GO:1902282; F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization; ISO:GO_Central. DR GO; GO:0001508; P:action potential; IMP:MGI. DR GO; GO:0002250; P:adaptive immune response; IMP:MGI. DR GO; GO:0006915; P:apoptotic process; IMP:MGI. DR GO; GO:0060922; P:atrioventricular node cell differentiation; IMP:MGI. DR GO; GO:0001568; P:blood vessel development; IMP:MGI. DR GO; GO:0070588; P:calcium ion transmembrane transport; IMP:MGI. DR GO; GO:0061762; P:CAMKK-AMPK signaling cascade; IMP:MGI. DR GO; GO:0060920; P:cardiac pacemaker cell differentiation; IMP:MGI. DR GO; GO:0008283; P:cell population proliferation; IMP:MGI. DR GO; GO:0072359; P:circulatory system development; IMP:MGI. DR GO; GO:0060976; P:coronary vasculature development; IMP:MGI. DR GO; GO:0051607; P:defense response to virus; IMP:MGI. DR GO; GO:0008340; P:determination of adult lifespan; IGI:MGI. DR GO; GO:0030010; P:establishment of cell polarity; IDA:MGI. DR GO; GO:0045444; P:fat cell differentiation; IMP:MGI. DR GO; GO:0015908; P:fatty acid transport; IMP:MGI. DR GO; GO:0048144; P:fibroblast proliferation; IGI:MGI. DR GO; GO:0010467; P:gene expression; IMP:MGI. DR GO; GO:0061535; P:glutamate secretion, neurotransmission; IGI:MGI. DR GO; GO:0007507; P:heart development; IMP:MGI. DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI. DR GO; GO:0006954; P:inflammatory response; IMP:MGI. DR GO; GO:0001822; P:kidney development; IEA:Ensembl. DR GO; GO:0098915; P:membrane repolarization during ventricular cardiac muscle cell action potential; ISO:GO_Central. DR GO; GO:0001774; P:microglial cell activation; IMP:MGI. DR GO; GO:0050877; P:nervous system process; IMP:MGI. DR GO; GO:0050905; P:neuromuscular process; IMP:MGI. DR GO; GO:0051402; P:neuron apoptotic process; IMP:MGI. DR GO; GO:0044546; P:NLRP3 inflammasome complex assembly; IDA:MGI. DR GO; GO:0038066; P:p38MAPK cascade; IMP:MGI. DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:MGI. DR GO; GO:1990573; P:potassium ion import across plasma membrane; ISO:GO_Central. DR GO; GO:0071805; P:potassium ion transmembrane transport; IMP:MGI. DR GO; GO:0006813; P:potassium ion transport; ISO:GO_Central. DR GO; GO:0009306; P:protein secretion; IMP:MGI. DR GO; GO:0150103; P:reactive gliosis; IMP:MGI. DR GO; GO:0008217; P:regulation of blood pressure; IMP:MGI. DR GO; GO:0002027; P:regulation of heart rate; IMP:MGI. DR GO; GO:0033198; P:response to ATP; IMP:MGI. DR GO; GO:0034097; P:response to cytokine; IMP:MGI. DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:MGI. DR GO; GO:0043330; P:response to exogenous dsRNA; IMP:MGI. DR GO; GO:0001666; P:response to hypoxia; IMP:MGI. DR GO; GO:0032868; P:response to insulin; IMP:MGI. DR GO; GO:0002931; P:response to ischemia; IMP:MGI. DR GO; GO:0032496; P:response to lipopolysaccharide; IMP:MGI. DR GO; GO:1904638; P:response to resveratrol; IMP:MGI. DR GO; GO:0006950; P:response to stress; IGI:MGI. DR GO; GO:0009410; P:response to xenobiotic stimulus; IMP:MGI. DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IMP:MGI. DR GO; GO:0055085; P:transmembrane transport; ISO:GO_Central. DR GO; GO:0019226; P:transmission of nerve impulse; IMP:MGI. DR GO; GO:0001944; P:vasculature development; IMP:MGI. DR GO; GO:0042311; P:vasodilation; IMP:MGI. DR GO; GO:0003229; P:ventricular cardiac muscle tissue development; IGI:MGI. DR FunFam; 1.10.287.70:FF:000050; ATP-sensitive inward rectifier potassium channel 11; 1. DR FunFam; 2.60.40.1400:FF:000001; G protein-activated inward rectifier potassium channel 2; 1. DR Gene3D; 1.10.287.70; -; 1. DR Gene3D; 2.60.40.1400; G protein-activated inward rectifier potassium channel 1; 1. DR InterPro; IPR014756; Ig_E-set. DR InterPro; IPR041647; IRK_C. DR InterPro; IPR016449; K_chnl_inward-rec_Kir. DR InterPro; IPR003278; K_chnl_inward-rec_Kir6.1. DR InterPro; IPR013518; K_chnl_inward-rec_Kir_cyto. DR InterPro; IPR040445; Kir_TM. DR PANTHER; PTHR11767:SF11; ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8; 1. DR PANTHER; PTHR11767; INWARD RECTIFIER POTASSIUM CHANNEL; 1. DR Pfam; PF01007; IRK; 1. DR Pfam; PF17655; IRK_C; 1. DR PIRSF; PIRSF005465; GIRK_kir; 1. DR PRINTS; PR01331; KIR61CHANNEL. DR PRINTS; PR01320; KIRCHANNEL. DR SUPFAM; SSF81296; E set domains; 1. DR SUPFAM; SSF81324; Voltage-gated potassium channels; 1. PE 1: Evidence at protein level; KW Ion channel; Ion transport; Membrane; Phosphoprotein; Potassium; KW Potassium transport; Reference proteome; Transmembrane; KW Transmembrane helix; Transport; Voltage-gated channel. FT CHAIN 1..424 FT /note="ATP-sensitive inward rectifier potassium channel 8" FT /id="PRO_0000154948" FT TOPO_DOM 1..69 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT TRANSMEM 70..94 FT /note="Helical; Name=M1" FT /evidence="ECO:0000250" FT TOPO_DOM 95..126 FT /note="Extracellular" FT /evidence="ECO:0000250" FT INTRAMEM 127..138 FT /note="Helical; Pore-forming; Name=H5" FT /evidence="ECO:0000250" FT INTRAMEM 139..145 FT /note="Pore-forming" FT /evidence="ECO:0000250" FT TOPO_DOM 146..154 FT /note="Extracellular" FT /evidence="ECO:0000250" FT TRANSMEM 155..176 FT /note="Helical; Name=M2" FT /evidence="ECO:0000250" FT TOPO_DOM 177..424 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT REGION 373..409 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 140..145 FT /note="Selectivity filter" FT /evidence="ECO:0000250" FT COMPBIAS 387..404 FT /note="Low complexity" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT SITE 170 FT /note="Role in the control of polyamine-mediated channel FT gating and in the blocking by intracellular magnesium" FT /evidence="ECO:0000250" FT MOD_RES 6 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:21183079" SQ SEQUENCE 424 AA; 47980 MW; BF02D1CC31970379 CRC64; MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI REQGRFLQDI FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD IYAYMEKGTM EKSGLESAVC VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE ECPLAITVLI LQNIVGLIIN AVMLGCIFMK TAQAHRRAET LIFSRHAVIA VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV VPIHQQDIPV DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLANQDL EVIVILEGVV ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA PRCSARELDE KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRNNSIRR NNSSLMVPKV QFMTPEGNQC PSES //