ID   SYT12_RAT               Reviewed;         421 AA.
AC   P97610;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   10-JUN-2026, entry version 161.
DE   RecName: Full=Synaptotagmin-12 {ECO:0000303|PubMed:17190793};
DE   AltName: Full=Synaptotagmin XII {ECO:0000250|UniProtKB:Q920N7};
DE            Short=SytXII {ECO:0000250|UniProtKB:Q920N7};
DE   AltName: Full=Synaptotagmin-related gene 1 protein {ECO:0000303|PubMed:8987811};
DE            Short=Srg1 {ECO:0000303|PubMed:8987811};
GN   Name=Syt12 {ECO:0000303|PubMed:17190793};
GN   Synonyms=Srg1 {ECO:0000303|PubMed:8987811}, Sytr1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RX   PubMed=8987811; DOI=10.1523/jneurosci.16-24-07832.1996;
RA   Thompson C.C.;
RT   "Thyroid hormone-responsive genes in developing cerebellum include a novel
RT   synaptotagmin and a hairless homolog.";
RL   J. Neurosci. 16:7832-7840(1996).
RN   [2]
RP   FUNCTION, INTERACTION WITH SYT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   PHOSPHORYLATION AT SER-97, AND MUTAGENESIS OF THR-87 AND SER-97.
RX   PubMed=17190793; DOI=10.1083/jcb.200607021;
RA   Maximov A., Shin O.H., Liu X., Suedhof T.C.;
RT   "Synaptotagmin-12, a synaptic vesicle phosphoprotein that modulates
RT   spontaneous neurotransmitter release.";
RL   J. Cell Biol. 176:113-124(2007).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97; SER-99 AND SER-214, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Synaptic vesicle phosphoprotein that enhances spontaneous
CC       neurotransmitter release but does not effect induced neurotransmitter
CC       release (PubMed:17190793). Unlike other synaptotagmins, it does not
CC       bind Ca(2+) or phospholipids (PubMed:17190793). Essential for mossy-
CC       fiber long-term potentiation in the hippocampus (By similarity).
CC       {ECO:0000250|UniProtKB:Q920N7, ECO:0000269|PubMed:17190793}.
CC   -!- SUBUNIT: Homodimer (By similarity). Can also form heterodimers (By
CC       similarity). Interacts with SYT1 (PubMed:17190793).
CC       {ECO:0000250|UniProtKB:Q9R0N7, ECO:0000269|PubMed:17190793}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC       vesicle membrane {ECO:0000269|PubMed:17190793}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in the brain, specifically in neurons of
CC       the cerebellum, cortex, hippocampus, olfactory bulb, brainstem and
CC       spinal cord (at protein level). {ECO:0000269|PubMed:17190793}.
CC   -!- PTM: Phosphorylation of Ser-97 is required for mossy-fiber long-term
CC       potentiation. {ECO:0000250|UniProtKB:Q920N7}.
CC   -!- SIMILARITY: Belongs to the synaptotagmin family. {ECO:0000305}.
CC   -!- CAUTION: Unlike classical synaptotagmins, lacks Ca(2+)-binding
CC       sequences and does not bind phospholipids.
CC       {ECO:0000269|PubMed:17190793}.
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DR   EMBL; U71294; AAC53019.1; -; mRNA.
DR   RefSeq; NP_620190.1; NM_138835.1.
DR   RefSeq; XP_038947967.1; XM_039092039.2.
DR   AlphaFoldDB; P97610; -.
DR   SMR; P97610; -.
DR   BioGRID; 251324; 1.
DR   FunCoup; P97610; 1130.
DR   IntAct; P97610; 1.
DR   MINT; P97610; -.
DR   STRING; 10116.ENSRNOP00000026153; -.
DR   iPTMnet; P97610; -.
DR   PhosphoSitePlus; P97610; -.
DR   PaxDb; 10116-ENSRNOP00000026153; -.
DR   ABCD; P97610; 1 sequenced antibody.
DR   Ensembl; ENSRNOT00000026152.8; ENSRNOP00000026153.3; ENSRNOG00000019306.8.
DR   Ensembl; ENSRNOT00055032390; ENSRNOP00055026212; ENSRNOG00055018999.
DR   Ensembl; ENSRNOT00060046435; ENSRNOP00060038595; ENSRNOG00060026807.
DR   Ensembl; ENSRNOT00065055899; ENSRNOP00065046035; ENSRNOG00065032488.
DR   GeneID; 191595; -.
DR   KEGG; rno:191595; -.
DR   UCSC; RGD:621876; rat.
DR   AGR; RGD:621876; -.
DR   CTD; 91683; -.
DR   RGD; 621876; Syt12.
DR   eggNOG; KOG1028; Eukaryota.
DR   GeneTree; ENSGT00940000158627; -.
DR   HOGENOM; CLU_053862_0_0_1; -.
DR   InParanoid; P97610; -.
DR   OMA; LQPFGGW; -.
DR   OrthoDB; 67700at2759; -.
DR   PhylomeDB; P97610; -.
DR   PRO; PR:P97610; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000019306; Expressed in cerebellum and 11 other cell types or tissues.
DR   ExpressionAtlas; P97610; baseline and differential.
DR   GO; GO:0070382; C:exocytic vesicle; IBA:GO_Central.
DR   GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; ISO:RGD.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0098793; C:presynapse; IDA:UniProtKB.
DR   GO; GO:0008021; C:synaptic vesicle; ISO:RGD.
DR   GO; GO:0030672; C:synaptic vesicle membrane; ISO:RGD.
DR   GO; GO:0061891; F:calcium ion sensor activity; IBA:GO_Central.
DR   GO; GO:0005544; F:calcium-dependent phospholipid binding; IBA:GO_Central.
DR   GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR   GO; GO:0060291; P:long-term synaptic potentiation; ISO:RGD.
DR   GO; GO:0099171; P:presynaptic modulation of chemical synaptic transmission; ISO:RGD.
DR   GO; GO:0017158; P:regulation of calcium ion-dependent exocytosis; IBA:GO_Central.
DR   GO; GO:0046928; P:regulation of neurotransmitter secretion; NAS:RGD.
DR   GO; GO:0048792; P:spontaneous exocytosis of neurotransmitter; IDA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   CDD; cd08406; C2B_Synaptotagmin-12; 1.
DR   FunFam; 2.60.40.150:FF:000080; Putative synaptotagmin-12; 1.
DR   FunFam; 2.60.40.150:FF:000129; Synaptotagmin 12; 1.
DR   Gene3D; 2.60.40.150; C2 domain; 2.
DR   InterPro; IPR000008; C2_dom.
DR   InterPro; IPR035892; C2_domain_sf.
DR   InterPro; IPR030537; Syt12_C2B.
DR   PANTHER; PTHR10024; SYNAPTOTAGMIN; 1.
DR   PANTHER; PTHR10024:SF252; SYNAPTOTAGMIN-12; 1.
DR   Pfam; PF00168; C2; 2.
DR   SMART; SM00239; C2; 2.
DR   SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 2.
DR   PROSITE; PS50004; C2; 2.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW   Synapse; Transmembrane; Transmembrane helix.
FT   CHAIN           1..421
FT                   /note="Synaptotagmin-12"
FT                   /id="PRO_0000183974"
FT   TOPO_DOM        1..18
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..421
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          152..272
FT                   /note="C2 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   DOMAIN          283..416
FT                   /note="C2 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT   MOD_RES         97
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000269|PubMed:17190793,
FT                   ECO:0007744|PubMed:22673903"
FT   MOD_RES         99
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         214
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MUTAGEN         87
FT                   /note="T->A: No effect on phosphorylation by PKA."
FT                   /evidence="ECO:0000269|PubMed:17190793"
FT   MUTAGEN         97
FT                   /note="S->A: Loss of phosphorylation by PKA. No effect on
FT                   localization to synapse. Inhibits the spontaneous release
FT                   of neurotransmitters."
FT                   /evidence="ECO:0000269|PubMed:17190793"
SQ   SEQUENCE   421 AA;  46608 MW;  C83F745FDD050897 CRC64;
     MAVDVTEYHL SVIKSPPGWE VGVYAAGALA LLGIAAVSLW KLWTSGSFPS PSPFPNYDYR
     YLQQKYGEAY VEAKLKRVPP WNAQRTTTRG PPSRKGSLSI EDTFESISEL GPLELMGREL
     DLAPYGTLRK SQSADSLNSI SSVSNTFGQD FTLGQVEVSM DYDGASHTLH VAVLQGKDLL
     EREEATFESC FMRVSLLPDE QIVGISRIQR NAYSIFFDEK FSVPLDPTAL EEKSLRFSVF
     GIDEDERNVS TGVVELKLSV LDLPLQPFSG WLYLQDQNKA ADAVGEILLS LSYLPTAERL
     TVVVVKAKNL IWTNDKTTAD PFVKVYLLQD GRKMSKKKTA VKRDDPNPVF NEAMIFSVPA
     IVLQDLSLRV TVAESSSDGR GDNVGHVIIG PGASGMGTTH WNQMLATLRR PVSMWHPVRR
     N
//