ID   PSME2_MOUSE             Reviewed;         239 AA.
AC   P97372; O35562;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   02-SEP-2026, entry version 187.
DE   RecName: Full=Proteasome activator complex subunit 2;
DE   AltName: Full=11S regulator complex subunit beta;
DE            Short=REG-beta;
DE   AltName: Full=Activator of multicatalytic protease subunit 2;
DE   AltName: Full=Proteasome activator 28 subunit beta;
DE            Short=PA28b;
DE            Short=PA28beta;
GN   Name=Psme2; Synonyms=Pa28b1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=B10.BR;
RX   PubMed=9162094; DOI=10.1007/s002510050246;
RA   Jiang H., Monaco J.J.;
RT   "Sequence and expression of mouse proteasome activator PA28 and the related
RT   autoantigen Ki.";
RL   Immunogenetics 46:93-98(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ;
RX   PubMed=9590240;
RA   Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M.;
RT   "Characterization of the mouse PA28 activator complex gene family: complete
RT   organizations of the three member genes and a physical map of the
RT   approximately 150-kb region containing the alpha- and beta-subunit genes.";
RL   J. Immunol. 160:4923-4935(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129;
RX   PubMed=9914329; DOI=10.1007/s002510050476;
RA   Li Y., Chambers J., Pang J., Ngo K., Peterson P.A., Leung W.P., Yang Y.;
RT   "Characterization of the mouse proteasome regulator PA28b gene.";
RL   Immunogenetics 49:149-157(1999).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ;
RX   PubMed=11345588; DOI=10.1007/s002510100308;
RA   Yawata M., Murata S., Tanaka K., Ishigatsubo Y., Kasahara M.;
RT   "Nucleotide sequence analysis of the ~35-kb segment containing interferon-
RT   gamma-inducible mouse proteasome activator genes.";
RL   Immunogenetics 53:119-129(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Embryo;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung,
RC   Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Implicated in immunoproteasome assembly and required for
CC       efficient antigen processing. The PA28 activator complex enhances the
CC       generation of class I binding peptides by altering the cleavage pattern
CC       of the proteasome.
CC   -!- SUBUNIT: Heterodimer of PSME1 and PSME2, which forms a hexameric ring.
CC   -!- INDUCTION: By interferon gamma.
CC   -!- SIMILARITY: Belongs to the PA28 family. {ECO:0000305}.
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DR   EMBL; U60329; AAC53296.1; -; mRNA.
DR   EMBL; AB007138; BAA28837.1; -; Genomic_DNA.
DR   EMBL; AF060195; AAC83999.1; -; Genomic_DNA.
DR   EMBL; AB053120; BAB47405.1; -; Genomic_DNA.
DR   EMBL; AK012344; BAB28175.1; -; mRNA.
DR   EMBL; BC005680; AAH05680.1; -; mRNA.
DR   EMBL; BC057859; AAH57859.1; -; mRNA.
DR   CCDS; CCDS49494.1; -.
DR   RefSeq; NP_001025026.1; NM_001029855.1.
DR   RefSeq; NP_035320.1; NM_011190.3.
DR   PDB; 5MSK; X-ray; 3.60 A; A/B/C/D/E/F/G=1-239.
DR   PDB; 5MX5; X-ray; 2.90 A; B/D/F/I/K/M=1-239.
DR   AlphaFoldDB; P97372; -.
DR   SMR; P97372; -.
DR   BioGRID; 202433; 39.
DR   BioGRID; 548745; 1.
DR   FunCoup; P97372; 1287.
DR   IntAct; P97372; 3.
DR   STRING; 10090.ENSMUSP00000100564; -.
DR   GlyGen; P97372; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; P97372; -.
DR   PhosphoSitePlus; P97372; -.
DR   SwissPalm; P97372; -.
DR   CPTAC; non-CPTAC-3942; -.
DR   jPOST; P97372; -.
DR   PaxDb; 10090-ENSMUSP00000100564; -.
DR   PeptideAtlas; P97372; -.
DR   ProteomicsDB; 301991; -.
DR   Pumba; P97372; -.
DR   DNASU; 19188; -.
DR   Ensembl; ENSMUST00000104958.2; ENSMUSP00000100564.2; ENSMUSG00000078153.4.
DR   Ensembl; ENSMUST00000161807.8; ENSMUSP00000123798.2; ENSMUSG00000079197.11.
DR   GeneID; 19188; -.
DR   KEGG; mmu:19188; -.
DR   KEGG; mmu:621823; -.
DR   UCSC; uc007tzg.1; mouse.
DR   AGR; MGI:1096365; -.
DR   CTD; 5721; -.
DR   MGI; MGI:1096365; Psme2.
DR   VEuPathDB; HostDB:ENSMUSG00000078153; -.
DR   VEuPathDB; HostDB:ENSMUSG00000079197; -.
DR   eggNOG; KOG4470; Eukaryota.
DR   GeneTree; ENSGT00950000183098; -.
DR   HOGENOM; CLU_062515_0_1_1; -.
DR   InParanoid; P97372; -.
DR   OMA; KKPPKCG; -.
DR   OrthoDB; 6591885at2759; -.
DR   PhylomeDB; P97372; -.
DR   Reactome; R-MMU-1236974; ER-Phagosome pathway.
DR   Reactome; R-MMU-1236978; Cross-presentation of soluble exogenous antigens (endosomes).
DR   Reactome; R-MMU-9907900; Proteasome assembly.
DR   Reactome; R-MMU-9912633; Antigen processing: Ub, ATP-independent proteasomal degradation.
DR   BioGRID-ORCS; 19188; 5 hits in 80 CRISPR screens.
DR   BioGRID-ORCS; 621823; 1 hit in 56 CRISPR screens.
DR   PRO; PR:P97372; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; P97372; protein.
DR   Bgee; ENSMUSG00000078153; Expressed in spleen and 55 other cell types or tissues.
DR   ExpressionAtlas; P97372; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0008537; C:proteasome activator complex; IEA:InterPro.
DR   GO; GO:0000502; C:proteasome complex; ISO:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; ISO:GO_Central.
DR   GO; GO:0019884; P:antigen processing and presentation of exogenous antigen; IMP:MGI.
DR   GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IBA:GO_Central.
DR   GO; GO:0061136; P:regulation of proteasomal protein catabolic process; ISO:GO_Central.
DR   FunFam; 1.20.120.180:FF:000002; Proteasome activator complex subunit 1; 1.
DR   FunFam; 1.20.5.120:FF:000002; proteasome activator complex subunit 2; 1.
DR   Gene3D; 1.20.120.180; Proteasome activator pa28, C-terminal domain; 1.
DR   Gene3D; 1.20.5.120; Proteasome activator pa28, N-terminal domain; 1.
DR   InterPro; IPR003186; PA28_C.
DR   InterPro; IPR036997; PA28_C_sf.
DR   InterPro; IPR036996; PA28_N_sf.
DR   InterPro; IPR009077; Proteasome_activ_PA28.
DR   InterPro; IPR003185; Proteasome_activ_PA28_N.
DR   InterPro; IPR036252; Proteasome_activ_sf.
DR   PANTHER; PTHR10660:SF6; PROTEASOME ACTIVATOR COMPLEX SUBUNIT 2; 1.
DR   PANTHER; PTHR10660; PROTEASOME REGULATOR PA28; 1.
DR   Pfam; PF02252; PA28_C; 1.
DR   Pfam; PF02251; PA28_N; 1.
DR   SUPFAM; SSF47216; Proteasome activator; 1.
DR   PDBsum; 5MSK; -.
DR   PDBsum; 5MX5; -.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Phosphoprotein; Proteasome; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UL46"
FT   CHAIN           2..239
FT                   /note="Proteasome activator complex subunit 2"
FT                   /id="PRO_0000161786"
FT   REGION          65..87
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        72..87
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UL46"
FT   MOD_RES         10
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UL46"
FT   CONFLICT        152
FT                   /note="A -> P (in Ref. 2; AAC53296)"
FT                   /evidence="ECO:0000305"
FT   HELIX           11..30
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           31..35
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           36..47
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   TURN            50..52
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           57..60
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           66..71
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           89..91
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           98..128
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           138..180
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   STRAND          181..184
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           186..222
FT                   /evidence="ECO:0007829|PDB:5MX5"
FT   HELIX           224..228
FT                   /evidence="ECO:0007829|PDB:5MX5"
SQ   SEQUENCE   239 AA;  27057 MW;  D8BB90F78CB0DD7C CRC64;
     MAKPCGVRLS GEARKQVDVF RQNLFQEADD FLCTFLPRKI ISLSQLLQED SLNVADLSSL
     RAPLDIPIPD PPPKDDEMET DKQEKKEVPK CGYLPGNEKL LALLALVKPE VWTLKEKCIL
     VITWIQHLIP KIEDGNDFGV AIQEKVLERV NAVKTKVEAF QTTISKYFSE RGDAVAKASK
     DTHVMDYRAL VHERDEAAYG ALRAMVLDLR AFYAELYHII SSNLEKIVNP KGEEKPSMY
//