ID P96834_MYCTU Unreviewed; 475 AA. AC P96834; F2GLV5; I6Y712; Q7DAD2; DT 01-MAY-1997, integrated into UniProtKB/TrEMBL. DT 01-MAY-1997, sequence version 1. DT 10-JUN-2026, entry version 161. DE RecName: Full=NAD(P) transhydrogenase subunit beta {ECO:0000256|ARBA:ARBA00014581, ECO:0000256|PIRNR:PIRNR000204}; DE EC=7.1.1.1 {ECO:0000256|ARBA:ARBA00012943, ECO:0000256|PIRNR:PIRNR000204}; DE AltName: Full=Nicotinamide nucleotide transhydrogenase subunit beta {ECO:0000256|PIRNR:PIRNR000204}; GN Name=pntB {ECO:0000313|EMBL:CCP42882.1}; GN OrderedLocusNames=Rv0157 {ECO:0000313|EMBL:CCP42882.1}; OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales; OC Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=83332 {ECO:0000313|EMBL:CCP42882.1, ECO:0000313|Proteomes:UP000001584}; RN [1] {ECO:0000313|EMBL:CCP42882.1, ECO:0000313|Proteomes:UP000001584} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 25618 / H37Rv {ECO:0000313|Proteomes:UP000001584}; RX PubMed=9634230; DOI=10.1038/31159; RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E.III., Tekaia F., Badcock K., RA Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., RA Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., RA Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., RA Barrell B.G.; RT "Deciphering the biology of Mycobacterium tuberculosis from the complete RT genome sequence."; RL Nature 393:537-544(1998). RN [2] {ECO:0007829|PubMed:21969609} RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21969609; DOI=10.1074/mcp.M111.011445; RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K., RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R., RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M., RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.; RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution RT mass spectrometry."; RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011). CC -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled to CC respiration and ATP hydrolysis and functions as a proton pump across CC the membrane. {ECO:0000256|ARBA:ARBA00003943, CC ECO:0000256|PIRNR:PIRNR000204}. CC -!- CATALYTIC ACTIVITY: CC Reaction=NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out); CC Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349; EC=7.1.1.1; CC Evidence={ECO:0000256|ARBA:ARBA00048202, CC ECO:0000256|PIRNR:PIRNR000204}; CC -!- SUBCELLULAR LOCATION: Cell inner membrane CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004429}. CC -!- SIMILARITY: Belongs to the PNT beta subunit family. CC {ECO:0000256|ARBA:ARBA00007919, ECO:0000256|PIRNR:PIRNR000204}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL123456; CCP42882.1; -; Genomic_DNA. DR RefSeq; NP_214671.1; NC_000962.3. DR RefSeq; WP_003899835.1; NC_000962.3. DR AlphaFoldDB; P96834; -. DR SMR; P96834; -. DR FunCoup; P96834; 2. DR STRING; 83332.Rv0157; -. DR PaxDb; 83332-Rv0157; -. DR DNASU; 886830; -. DR GeneID; 886830; -. DR KEGG; mtu:Rv0157; -. DR KEGG; mtv:RVBD_0157; -. DR PATRIC; fig|83332.111.peg.183; -. DR TubercuList; Rv0157; -. DR eggNOG; COG1282; Bacteria. DR InParanoid; P96834; -. DR OrthoDB; 9763786at2; -. DR PhylomeDB; P96834; -. DR Proteomes; UP000001584; Chromosome. DR AbasyAtlas; P96834; -. DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0008750; F:proton-translocating NAD(P)+ transhydrogenase activity; IBA:GO_Central. DR GO; GO:0006740; P:NADPH regeneration; IBA:GO_Central. DR GO; GO:0120029; P:proton export across plasma membrane; IBA:GO_Central. DR FunFam; 3.40.50.1220:FF:000002; NAD(P) transhydrogenase subunit beta; 1. DR Gene3D; 3.40.50.1220; TPP-binding domain; 1. DR InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom. DR InterPro; IPR012136; NADH_DH_b. DR InterPro; IPR034300; PNTB-like. DR PANTHER; PTHR44758; NAD(P) TRANSHYDROGENASE SUBUNIT BETA; 1. DR PANTHER; PTHR44758:SF1; NAD(P) TRANSHYDROGENASE SUBUNIT BETA; 1. DR Pfam; PF02233; PNTB; 1. DR PIRSF; PIRSF000204; PNTB; 1. DR SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1. PE 1: Evidence at protein level; KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519, KW ECO:0000256|PIRNR:PIRNR000204}; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, KW ECO:0000256|PIRNR:PIRNR000204}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR000204}; KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|PIRNR:PIRNR000204}; KW NADP {ECO:0000256|ARBA:ARBA00022857, ECO:0000256|PIRNR:PIRNR000204}; KW Reference proteome {ECO:0000313|Proteomes:UP000001584}; KW Translocase {ECO:0000256|ARBA:ARBA00022967, ECO:0000256|PIRNR:PIRNR000204}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}. FT TRANSMEM 6..23 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 35..53 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 59..76 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 88..105 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 125..149 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 170..191 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 197..219 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 226..246 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 252..270 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 8..470 FT /note="NADP transhydrogenase beta-like" FT /evidence="ECO:0000259|Pfam:PF02233" SQ SEQUENCE 475 AA; 48858 MW; 65131735A90D9F02 CRC64; MNLHYLVEIL YIISFSLFIY GLMGLTGPKT AVRGNLIAAA GMTIAVAATL VMIRHTSQWP LIIAGLVVGV VLGVPPARLT KMTAMPQLVA FFNGVGGGTV ALIALSEFID TTGFSAFQHG ESPTVHIVVA SLFAAIIGSI SFWGSIVAFG KLQEIISGRP IGLGKAQQPI NLLLLAVAVA AAVVIGLHAH PGSGGVALWW MIGLLVAAGV LGLMVVLPIG GADMPVVISM LNAMTGLSAA AAGLALNNTA MIVAGMIVGA SGSILTNLMA KAMNRSIPAI VAGGFGGGGV APSGGGDDKH VKATSAADAA IQMAYANQVI VVPGYGLAVA QAQHAVKDLA TLLEDRGVPV KYAIHPVAGR MPGHMNVLLA EAEVDYDAMK DMDDINDEFA RTDVTIVIGA NDVTNPAARN ETSSPIYGMP ILNVDKSRSV IVLKRSMNSG FAGIDNPLFY ADGTTMLFGD AKKSVTEVSE ELKAL //